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Hip1 (NM_146001) Mouse Tagged ORF Clone
CAT＃: MR211131
- TrueORF®
Hip1 (Myc-DDK-tagged) - Mouse huntingtin interacting protein 1 (Hip1)
ORF Plasmid: tGFP
"NM_146001" in other vectors (4)
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CNY 7,168.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610109B09Rik; A930014B11Rik; E130315I21Rik; mKIAA4113 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR211131 representing NM_146001
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCGAATGGCCAGCTCCATGAAGCAGGTATCCAACCCGCTGCCCAAAGTGTTGAGCCGCCGCGGAG TTGGTGCTGGGATGGAGGCTGCGGAGCGCGAGAGCTTCGAGCGGACTCAGACGGTCAGCGTCAATAAGGC CATTAATACGCAGGAAGTGGCCGTGAAAGAGAAACATGCCAGGACGTGTATTCTGGGCACCCACCATGAG AAAGGAGCGCAGACCTTTTGGTCTGTGGTCAACCGACTGCCTCTCTCCAGCAACGCCATGCTGTGCTGGA AGTTTTGTCACGTGTTCCACAAACTTCTTCGAGATGGACATCCGAACGTGCTGAAAGACTCTCTGAGATA CAAAAATGAGTTAAGCGACATGAGCAGGATGTGGGGCCACCTGAGCGAGGGGTATGGACAGCTTTGCAGC ATCTACCTCAAACTGCTGAGAACAAGGATGGAGTACCACACCAAAAATCCCAGGTTCCCGGGCAACCTCC AGATGAGTGACCGACAGCTGGATGAGGCCGGAGAAAGTGACGTTAACAACTTTTTTCAGCTCACAGTGGA GATGTTTGATTACCTGGAGTGTGAACTGAACCTCTTCCAGACTGTGTTCAACTCCTTGGACATGTCCCGT TCGGTGTCCGTGACCACGGCTGGCCAGTGCCGCCTCGCACCGCTCATCCAGGTCATCCTGGACTGCAGTC ACCTCTATGACTACACTGTCAAGCTGCTCTTCAAGCTCCACTCCTGTCTTCCAGCTGACACCCTGCAGGG CCACCGAGACCGCTTCATGGAGCAGTTCACAAAGTTGAAAGATCTGTTTCAGCGCTCCAGCAACCTACAG TACTTCAAGCGGCTCATTCAGATCCCCCAGCTGCCCGAGAATCCACCCAACTTCCTACGAGCCTCGGCCC TGTCAGAGCACATCAGTCCTGTGGTGGTGATCCCGGCAGAGGTGTCATCCCCAGACAGTGAGCCTGTCCT GGAGAAGGATGACCTCATGGACATGGACGCCTCCCAGCAGACTTTGTTTGACAACAAGTTTGATGACGTC TTTGGCAGCTCATTGAGCAGCGACCCTTTCAATTTCAACAATCAAAATGGCGTGAACAAGGACGAGAAGG ACCACTTGATTGAACGCCTGTACAGAGAGATCAGTGGACTGACAGGGCAGCTGGACAACATGAAGATTGA GAGCCAGCGGGCCATGCTGCAGCTGAAGGGTCGAGTGAGTGAGCTGGAGGCAGAGCTAGCAGAGCAGCAG CACTTGGGCCGGCAGGCTATGGATGACTGCGAGTTCCTGCGCACTGAGCTGGATGAACTGAAGAGGCAGC GAGAGGACACGGAGAAGGCACAGCGCAGCCTGACTGAGATAGAAAGAAAGGCCCAGGCTAATGAACAGAG GTATAGCAAGTTAAAAGAGAAGTACAGTGAACTGGTGCAGAACCATGCTGACCTGCTGCGGAAGAACGCA GAGGTGACCAAACAGGTGTCCGTGGCCCGGCAAGCCCAGGTGGATTTGGAAAGAGAGAAAAAAGAGCTAG CAGATTCCTTTGCACGGACTCAAGAGCAACAGGATGTTCTAGAGAACCTGAAGCATGAACTGGCCACCAG CAGACAGGAGCTGCAGGTCCTCCACAGCAACCTGGAAACCTCTGCCCAGTCAGAAGCGAAATGGCTGACA CAGATCGCCGAGTTGGAGAAGGAACAAGGCAGCTTGGCGACTGTTGCAGCTCAGAGAGAGGAAGAGTTAT CAGCCCTCCGAGACCAGCTGGAAAGCACCCAGATCAAGCTGGCTGGGGCCCAGGAATCCATGTGCCAGCA GGTGAAGGACCAGAGGAAAACCCTCTTGGCAGGGATCAGGAAGGCTGCGGAGCGTGAGATACAGGAGGCG CTGAGCCAGCTTGAGGAACCCACCCTCATCAGCTGTGCAGGATCCACAGATCACCTTCTCTCCAAAGTCA GCTCCGTTTCCAGCTGCCTCGAGCAACTGGAAAAGAACGGCAGCCAGTATCTGGCCTGCCCAGAAGATAT TAGTGAGCTTCTGCACTCGATCACCCTGCTTGCCCACTTGACCGGTGACACTATCATCCAGGGGAGTGCC ACCAGCCTCCGGGCCCCACCGGAGCCAGCCGACTCGCTGACGGAGGCCTGTAGGCAGTATGGCAGAGAAA CCCTGGCCTATCTGTCCTCCCTGGAGGAAGAGGGAACTATGGAGAATGCTGACGTCACAGCCCTTAGGAA CTGCCTCAGCAGGGTCAAGACCCTTGGCGAGGAGCTGCTGCCCAGGGGCCTGGACATCAAGCAGGAAGAG CTGGGTGACCTGGTGGACAAGGAGATGGCAGCCACTTCAGCTGCCATTGAAGCTGCCACCACCCGGATAG AGGAAATTCTCAGTAAGTCCCGAGCAGGAGACACGGGAGTCAAGCTGGAGGTGAATGAGAGGATCCTGGG TTCCTGTACCAGCCTGATGCAGGCCATCAAGGTGCTCGTTGTGGCCTCCAAGGACCTCCAGAAGGAGATA GTGGAGAGTGGCAGGGGTACTGCATCCCCTAAAGAATTTTACGCCAAGAACTCTCGGTGGACGGAAGGGC TGATATCCGCCTCCAAAGCTGTTGGTTGGGGAGCTACCATCATGGTGGATGCTGCTGATCTTGTGGTCCA AGGCAAAGGGAAGTTCGAGGAGCTGATGGTGTGTTCACGCGAGATTGCTGCCAGTACTGCCCAGCTCGTG GCTGCATCCAAGGTGAAAGCGAACAAGGGCAGCCTCAATCTGACCCAGCTGCAGCAGGCCTCTCGAGGAG TGAACCAGGCCACAGCCGCTGTGGTGGCCTCAACCATTTCTGGCAAATCTCAGATTGAGGAAACAGACAG TATGGACTTCTCAAGCATGACACTGACCCAGATCAAGCGCCAGGAGATGGATTCCCAGGTTAGGGTGCTG GAGCTGGAAAATGACCTGCAGAAGGAGCGTCAGAAACTAGGAGAGCTACGGAAGAAACACTACGAGCTGG CGGGCGTGGCTGAGGGCTGGGAGGAAGGGACAGAAGCATCACCGTCTACTGTCCAAGAAGCAATACCGGA CAAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR211131 representing NM_146001
Red=Cloning site Green=Tags(s) MDRMASSMKQVSNPLPKVLSRRGVGAGMEAAERESFERTQTVSVNKAINTQEVAVKEKHARTCILGTHHE KGAQTFWSVVNRLPLSSNAMLCWKFCHVFHKLLRDGHPNVLKDSLRYKNELSDMSRMWGHLSEGYGQLCS IYLKLLRTRMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSR SVSVTTAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFQRSSNLQ YFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEVSSPDSEPVLEKDDLMDMDASQQTLFDNKFDDV FGSSLSSDPFNFNNQNGVNKDEKDHLIERLYREISGLTGQLDNMKIESQRAMLQLKGRVSELEAELAEQQ HLGRQAMDDCEFLRTELDELKRQREDTEKAQRSLTEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNA EVTKQVSVARQAQVDLEREKKELADSFARTQEQQDVLENLKHELATSRQELQVLHSNLETSAQSEAKWLT QIAELEKEQGSLATVAAQREEELSALRDQLESTQIKLAGAQESMCQQVKDQRKTLLAGIRKAAEREIQEA LSQLEEPTLISCAGSTDHLLSKVSSVSSCLEQLEKNGSQYLACPEDISELLHSITLLAHLTGDTIIQGSA TSLRAPPEPADSLTEACRQYGRETLAYLSSLEEEGTMENADVTALRNCLSRVKTLGEELLPRGLDIKQEE LGDLVDKEMAATSAAIEAATTRIEEILSKSRAGDTGVKLEVNERILGSCTSLMQAIKVLVVASKDLQKEI VESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATIMVDAADLVVQGKGKFEELMVCSREIAASTAQLV AASKVKANKGSLNLTQLQQASRGVNQATAAVVASTISGKSQIEETDSMDFSSMTLTQIKRQEMDSQVRVL ELENDLQKERQKLGELRKKHYELAGVAEGWEEGTEASPSTVQEAIPDKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_146001 |
| ORF Size | 3087 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_146001.2, NP_666113.2 |
| RefSeq Size | 7883 bp |
| RefSeq ORF | 3090 bp |
| Locus ID | 215114 |
| UniProt ID | Q8VD75 |
| MW | 115.7 kDa |
| Gene Summary | Plays a role in clathrin-mediated endocytosis and trafficking (PubMed:11577110). Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner (PubMed:12839988, PubMed:17329427). Regulates presynaptic nerve terminal activity (PubMed:17928447). Enhances androgen receptor (AR)-mediated transcription (By similarity). May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway (By similarity). Binds 3-phosphoinositides (via ENTH domain) (By similarity). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis (By similarity). May play a functional role in the cell filament networks (By similarity). May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors (PubMed:11604514, PubMed:14998932, PubMed:16967501, PubMed:17928447).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203942 | Hip1 (untagged) - Mouse huntingtin interacting protein 1 (Hip1), (10ug) |
CNY 2,000.00 |
|
| MG211131 | Hip1 (tGFP-tagged) - Mouse huntingtin interacting protein 1 (Hip1) |
CNY 9,220.00 |
|
| MR211131L3 | Lenti ORF clone of Hip1 (Myc-DDK-tagged) - Mouse huntingtin interacting protein 1 (Hip1) |
CNY 10,260.00 |
|
| MR211131L4 | Lenti ORF clone of Hip1 (mGFP-tagged) - Mouse huntingtin interacting protein 1 (Hip1) |
CNY 10,260.00 |
