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Pign (BC021148) Mouse Tagged ORF Clone
CAT＃: MR210838
- TrueORF®
Pign (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793)
ORF Plasmid: tGFP
"BC021148" in other vectors (4)
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CNY 6,048.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gm20308; PIG-N |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210838 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGTTTTTTGCTTTGGGATTGCTTATCCATTTTGTGTTCTTCGCTTCCATCTTTGACATTTATT TTACATCTCCTTTGGTTCATGGAATGACTCCCCAGTTTACACCTTTGCCTCCTCCAGCAAAAAGATTAGT GCTATTTGTTGCTGATGGCCTGAGAGCAGATACTCTTTATGAATTAGATGAAGATGGAAACTCCAGAGCA CCTTTCATTAGGAATGTTATAATTCATGAAGGCAGCTGGGGAGTATCTCACACACGTGTGCCAACAGAAT CTCGGCCTGGCCATGTAGCCTTGATAGCTGGATTTTATGAGGATGTCAGTGCAGTTGCCAAAGGATGGAA AGAAAATCCTGTAGAATTTGACTCTCTTTTTAATGAAAGCAAGTACACGTGGAGCTGGGGGAGTCCAGAT ATCTTGCCCATGTTTGCCAAAGGTGCTAGTGGTGACCATGTCTATACATATAGTTACGATGCTCAAAGAG AAGATTTTGGTGCCCACGATGCCACAAAACTGGATACGTGGGTTTTTGATAAAGTTAAGGACTTCTTTGA TGCTGCAAGAAACAACCAATCTTTGTTCACAAAAGTAAATGAAGAAAAAGTAGTTTTTTTCTTACATTTA TTGGGAATAGATACAAATGGACATGCTCATCGACCATCGTCAAGGGAATATAAGGACAATATTAAAAAAG TTGATGATGGAGTGAAGGAAATCGTATCAATTTTCAAGCATTTTTATGGAGATGATGGGAAAACAGCATT TATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCGTCAGAGACTTTA ACTCCGTTCGTTACTTGGGGAGCTGGAATCAAATTTCCTCAAAATGTATCAGCTCAGCAATATGATGATG AGTTTTTAAAAGAATGGAGATTGGAAAACTGGAAAAGGAGAGATGTCAATCAGGCTGATATTGCACCTCT GATGGCATCCCTTATTGGAGTCCCCTTTCCTCTTAATTCGGTGGGAATACTTCCTGTGGGTTATCTTAAC AACACTGGTCTCTTCAAAGCAGAGAGCATGTTTACCAATGCAGTACAGATTCTTGAACAGTTCAAGGTGA AAATGACTCAGAAAAAAGAAGCTACTTTACCGTTTTTGTTTACACCATTTAAGTTGCTTTCTGATTCTCA ACAACTGGACATTTTAAGAAAAGCAAGATCTTACATAAAACAAGAAAAATTTGATGAAGTAGTGTCCCTC TGTGAGGAGCTGATTGACCTCGCCCTGAGAGGATTGTCCTATTATCATACTTATGACAGACTGTTTCTGG GCATCAATGTTGCAGTTGGTTTTGTGGGATGGATGTCTTATACTTCTTTGTTAATCATCAAGTCGCATTC TAATATCCCAAAAGGCACTAGGAAGGAAGGTAAGAAGCCACACTGTCTCCTGCTTTATAGCTTCATAGCT ACTGGTGTCTTAGTAGCATGCTTCCTGATGATCCAAGCCTGTCCCTGGACTTACTATGTGTACTGCTTGC TGCCAGTGCCTATATGGTATGCAGTTCTAAGAGAACATGAAGTTATTCAAGACCTGGTTGAATCACTATT GACTTTTCCTCGGAGCCATTTTGTTGCCTACTTGCTTGTCTTTACCTTGGGTATTGAAGTACTGGTGCTC AGTTTCTTCTACCGCTACATGTTGACAGCGGGACTGATTGTGTTTGCAGGCTGGCCGTTTCTCACTCAGC TGTGGACCCGAGCAAAGATCACCTTCCTGAGTTGGGCTTTCTTCTCTTTGCTTCTGGCAGTGTTCCCACT GATGCCTGTTGTGGGTAGAAAGCCAAACCTTTCTCTAGTAATGGGTGCTGGATTCCTGGTTCTTCTGCTG TCGCTGGCTGTTGTAACAACTCTCGGAAAAAGAAACATTAAATTGGTAAAGGGCGAGCTGTTGGTACTTC TGTTACAGATGCTGAGTACAGTGCTCTCTATGTATGTGGTGTATAGCACCCACCACAGTCTGTTAAAGAA AGAAGGCCTGCCTCTTATGAATCAGATTGTGAGCTGGGCAACATTAGCCTCTTCTCTGGTGGCACCACTC CTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCC TAAGCACTGGGTATGAAGCTCTCTTTCCTTTAGTGCTGTCCTGTTTGATGTTTGTCTGGATCCAAGTGGA ACAAGAGACCCTTCAACAGCCTGGTGTTTCTTGTAAACAGAAGCTCACCAGCATCCAGTTCACCTGTGAC ACTGATATAGCTCAGTTCCGACAGCTCTGTCCGGATGACATCCGTAGGGCCTTTTTCCTCATATTGTTGA TCTTTCCTGTATTCTTAAGACTATTCGACTACAAAAGAATAAGGATTCTGTTAGCCCAAGAAAATTATCA TAGGTCATTAAAGGCTGCCTGGGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210838 protein sequence
Red=Cloning site Green=Tags(s) MLLFFALGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRA PFIRNVIIHEGSWGVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPD ILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHL LGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSETL TPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPLNSVGILPVGYLN NTGLFKAESMFTNAVQILEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSL CEELIDLALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYSFIA TGVLVACFLMIQACPWTYYVYCLLPVPIWYAVLREHEVIQDLVESLLTFPRSHFVAYLLVFTLGIEVLVL SFFYRYMLTAGLIVFAGWPFLTQLWTRAKITFLSWAFFSLLLAVFPLMPVVGRKPNLSLVMGAGFLVLLL SLAVVTTLGKRNIKLVKGELLVLLLQMLSTVLSMYVVYSTHHSLLKKEGLPLMNQIVSWATLASSLVAPL LSSTALSQRLASILLSLMSTYLLLSTGYEALFPLVLSCLMFVWIQVEQETLQQPGVSCKQKLTSIQFTCD TDIAQFRQLCPDDIRRAFFLILLIFPVFLRLFDYKRIRILLAQENYHRSLKAAWEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC021148 |
| ORF Size | 2478 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC021148, AAH21148 |
| RefSeq Size | 4038 bp |
| RefSeq ORF | 2480 bp |
| Locus ID | 27392 |
| MW | 93.8 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200937 | Pign (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793), (10ug) |
CNY 6,056.00 |
|
| MG210838 | Pign (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793) |
CNY 5,230.00 |
|
| MR210838L3 | Lenti ORF clone of Pign (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793) |
CNY 6,650.00 |
|
| MR210838L4 | Lenti ORF clone of Pign (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793) |
CNY 6,650.00 |
