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Eya1 (NM_010164) Mouse Tagged ORF Clone
CAT＃: MR209161
- TrueORF®
Eya1 (Myc-DDK-tagged) - Mouse eyes absent 1 homolog (Drosophila) (Eya1)
ORF Plasmid: tGFP
"NM_010164" in other vectors (3)
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CNY 4,360.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bor |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR209161 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGATCTAACCAGCCCGCATAGCCGACTGAGTGGTAGTAGCGAATCCCCCAGTGGTCCCAAACTCG ATAGCTCTCATATAAATAGTACTTCCATGACTCCCAATGGCACCGAAGTTAAAACAGAGCCAATGAGCAG CAGTGAAATAGCTTCAACAGCAGCAGACGGGTCTTTAGACAGTTTCTCAGGTTCAGCTCTCGGAAGCAGC AGCTTTAGTCCAAGACCAGCTCACCCGTTCTCTCCACCACAGATTTATCCTTCCAACAGATCATACCCAC ATATTCTCCCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGGAATGCA ACAAGCCACAGCCTACGCCACGTACCCACAGCCTGGACAGCCCTATGGAATTTCCTCCTATGGCATCAAG ACGGAAAGTGGATTGTCACAGTCTCAGTCACCTGGACAGACGGGATTTCTTAGCTATGGCACAAGCTTTG GTACCCCTCAACCTGGACAGGCACCGTACAGCTACCAGATGCAAGGTAGCAGCTTTACCACGTCATCAGG ATTATATTCAGGAAATAATTCACTCACCAACTCCTCCGGATTCAACAGTTCACAGCAGGACTATCCGTCT TATCCCGGCTTTGGCCAGGGTCAGTACGCACAGTATTATAACAGCTCGCCGTATCCAGCACACTACATGA CGAGCAGTAACACCAGCCCGACCACACCGTCCACCAATGCCACTTACCAACTCCAGGAACCACCTTCTGG CGTCACAAGTCAGGCGGTCACAGACCCCACAGCAGAGTACAGTACAATCCACAGTCCTTCCACACCCATT AAAGAGACTGACTCCGAGCGGCTGCGTCGAGGTTCAGATGGGAAGTCACGTGGCCGAGGCAGAAGAAACA ATAATCCCTCCCCTCCCCCGGATTCTGACCTTGAGAGAGTGTTCATCTGGGACCTGGACGAGACCATCAT TGTTTTCCACTCCTTGCTCACGGGGTCCTACGCCAACAGATACGGGAGGGATCCACCTACTTCTGTTTCC CTGGGACTACGAATGGAAGAGATGATTTTCAACTTGGCAGACACACATCTATTTTTCAATGACCTAGAAG AGTGTGACCAAGTCCATATAGATGATGTTTCATCAGACGACAACGGCCAGGACCTGAGCACATACAACTT TGGAACAGATGGCTTTCCTGCTGCAGCCACCAGTGCTAATTTATGCCTGGCAACTGGTGTCCGAGGTGGT GTGGACTGGATGCGGAAACTGGCCTTCCGCTACAGACGAGTAAAAGAGATCTACAACACCTACAAAAACA ACGTGGGAGGTCTGCTTGGCCCAGCTAAGAGGGAAGCCTGGCTCCAGCTGAGGGCTGAGATTGAGGCACT CACAGACTCCTGGCTGACCCTGGCCCTGAAGGCCCTCTCCCTCATCCACTCCCGGACGAACTGTGTGAAT ATTTTAGTAACAACTACGCAGCTCATCCCAGCATTGGCAAAAGTCCTGCTATATGGATTAGGAATTGTGT TTCCAATAGAAAATATTTACAGTGCAACTAAAATAGGAAAGGAAAGCTGTTTTGAGAGGATAATCCAAAG GTTTGGAAGGAAAGTGGTATACGTTGTCATAGGAGATGGTGTGGAAGAAGAGCAAGGGGCAAAAAAGCAT GCTATGCCCTTCTGGAGGGTCTCCAGTCACTCGGACCTCATGGCACTGCATCATGCCTTGGAATTAGAGT ACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR209161 protein sequence
Red=Cloning site Green=Tags(s) MQDLTSPHSRLSGSSESPSGPKLDSSHINSTSMTPNGTEVKTEPMSSSEIASTAADGSLDSFSGSALGSS SFSPRPAHPFSPPQIYPSNRSYPHILPTPSSQTMAAYGQTQFTTGMQQATAYATYPQPGQPYGISSYGIK TESGLSQSQSPGQTGFLSYGTSFGTPQPGQAPYSYQMQGSSFTTSSGLYSGNNSLTNSSGFNSSQQDYPS YPGFGQGQYAQYYNSSPYPAHYMTSSNTSPTTPSTNATYQLQEPPSGVTSQAVTDPTAEYSTIHSPSTPI KETDSERLRRGSDGKSRGRGRRNNNPSPPPDSDLERVFIWDLDETIIVFHSLLTGSYANRYGRDPPTSVS LGLRMEEMIFNLADTHLFFNDLEECDQVHIDDVSSDDNGQDLSTYNFGTDGFPAAATSANLCLATGVRGG VDWMRKLAFRYRRVKEIYNTYKNNVGGLLGPAKREAWLQLRAEIEALTDSWLTLALKALSLIHSRTNCVN ILVTTTQLIPALAKVLLYGLGIVFPIENIYSATKIGKESCFERIIQRFGRKVVYVVIGDGVEEEQGAKKH AMPFWRVSSHSDLMALHHALELEYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010164 |
| ORF Size | 1758 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010164.2, NP_034294.2 |
| RefSeq Size | 4354 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 14048 |
| MW | 63.7 kDa |
| Gene Summary | Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:10490620). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed:19234442). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis (PubMed:14628042). Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears (PubMed:10471511). Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2 (PubMed:17098221).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209161 | Eya1 (tGFP-tagged) - Mouse eyes absent 1 homolog (Drosophila) (Eya1) |
CNY 5,040.00 |
|
| MR209161L3 | Lenti ORF clone of Eya1 (Myc-DDK-tagged) - Mouse eyes absent 1 homolog (Drosophila) (Eya1) |
CNY 6,460.00 |
|
| MR209161L4 | Lenti ORF clone of Eya1 (mGFP-tagged) - Mouse eyes absent 1 homolog (Drosophila) (Eya1) |
CNY 6,460.00 |
