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Cyp1b1 (NM_009994) Mouse Tagged ORF Clone
CAT＃: MR208663
- TrueORF®
Cyp1b1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 1, subfamily b, polypeptide 1 (Cyp1b1)
ORF Plasmid: tGFP
"NM_009994" in other vectors (3)
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CNY 4,048.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CP1B; P4501b1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208663 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCAGCCTTAGTGCAGACAGTCCACAGCAGCTGAGCTCGCTGTCTACCCAGCAGACCACTCTTC TGCTACTCTTCTCCGTCCTGGCCGCCGTGCACTTAGGCCAGTGGCTGCTGCGACAGTGGCAACGGAAACC GTGGTCCTCGCCCCCAGGTCCCTTTCCTTGGCCACTGATCGGAAACGCGGCGGCTGTTGGCCAGGCGTCG CACTTGTACTTCGCTCGCCTTGCAAGGCGCTATGGCGACGTTTTCCAGATCCGTCTGGGCAGCTGTCCCG TGGTGGTGCTGAATGGAGAGAGTGCCATCCACCAGGCCCTGGTGCAGCAGGGCAGCATCTTCGCGGACCG GCCGCCCTTCGCCTCTTTCCGTGTGGTGTCTGGTGGCCGCAGTCTGGCGTTCGGTCACTACTCTGAGCAC TGGAAGACGCAGCGACGCTCGGCCTATAGCACGATGCGTGCTTTCTCCACGCGCCACCCGCGCAGTCGCG GTCTTCTCGAGGGCCACGCGCTGGCAGAGGCTCGAGAATTGGTGGCAGTGCTGGTGCGGCGCTGTGCGGG CGGCGCCTTCCTCGATCCAACGCAGCCGGTCATTGTGGCGGTGGCCAACGTCATGAGCGCTGTGTGCTTC GGCTGTCGGTACAACCACGACGATGCGGAGTTCCTAGAGCTGCTCAGCCACAATGAGGAGTTCGGGCGCA CAGTGGGTGCGGGCAGCCTGGTGGATGTGCTGCCCTGGCTGCAGCTATTTCCCAACCCGGTGCGCACCAC CTTCCGCAAGTTCGAGCAGCTCAACCGCAACTTCAGCAACTTCGTTCTGGACAAGTTCCTGAGGCACCGC GAAAGCCTGGTGCCCGGGGCTGCTCCTCGAGACATGACGGACGCCTTCATCCTCTCTGCCGAAAAGAAAG CGTCTGGGGCCCCTGGCGACGATTCCTCCGGGCTGGACTTGGAGGATGTGCCTGCCACTATTACGGACAT CTTCGGAGCCAGCCAGGACACCCTTTCCACCGCGCTGCTGTGGCTGCTCATCCTCTTTACCAGATACCCG GATGTTCAGGCCCGCGTGCAGGCTGAGTTGGACCAGGTTGTGGGGAGGGACCGCCTGCCCTGCATGAGTG ACCAGCCCAACCTGCCATATGTCATGGCTTTTCTTTATGAATCAATGCGATTCTCCAGCTTTTTGCCTGT CACCATTCCACACGCCACCACCGCCAACACCTTTGTTTTAGGTTACTACATCCCCAAGAATACGGTCGTT TTTGTTAACCAGTGGTCTGTGAATCATGACCCAACCAAGTGGCCTAACCCAGAGGACTTTGATCCAGCCC GCTTCCTGGACAAGGACGGCATCATTAACAAGGCGCTAGCCAGCAGTGTGATGATATTCTCAGTGGGCAA ACGGAGGTGCATCGGTGAGGAACTGTCTAAGATGCTTCTGTTTCTCTTCATCTCCATCCTCGCTCATCAG TGCAATTTCAAGGCTAACCAAAATGAGTCCTCAAACATGAGTTTCAGTTATGGCCTGACCATTAAGCCCA AGTCGTTTAGAATCCATGTGTCTCTCAGAGAGTCGATGGAACTCCTGGATAATGCTGTTAAAAAGCTGCA AACTGAGGAAGGCTGCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208663 protein sequence
Red=Cloning site Green=Tags(s) MATSLSADSPQQLSSLSTQQTTLLLLFSVLAAVHLGQWLLRQWQRKPWSSPPGPFPWPLIGNAAAVGQAS HLYFARLARRYGDVFQIRLGSCPVVVLNGESAIHQALVQQGSIFADRPPFASFRVVSGGRSLAFGHYSEH WKTQRRSAYSTMRAFSTRHPRSRGLLEGHALAEARELVAVLVRRCAGGAFLDPTQPVIVAVANVMSAVCF GCRYNHDDAEFLELLSHNEEFGRTVGAGSLVDVLPWLQLFPNPVRTTFRKFEQLNRNFSNFVLDKFLRHR ESLVPGAAPRDMTDAFILSAEKKASGAPGDDSSGLDLEDVPATITDIFGASQDTLSTALLWLLILFTRYP DVQARVQAELDQVVGRDRLPCMSDQPNLPYVMAFLYESMRFSSFLPVTIPHATTANTFVLGYYIPKNTVV FVNQWSVNHDPTKWPNPEDFDPARFLDKDGIINKALASSVMIFSVGKRRCIGEELSKMLLFLFISILAHQ CNFKANQNESSNMSFSYGLTIKPKSFRIHVSLRESMELLDNAVKKLQTEEGCK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009994 |
| ORF Size | 1632 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009994.1, NP_034124.1 |
| RefSeq Size | 5128 bp |
| RefSeq ORF | 1632 bp |
| Locus ID | 13078 |
| UniProt ID | Q64429 |
| MW | 60.5 kDa |
| Gene Summary | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (By similarity). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (By similarity). Exhibits catalytic activity for the formation of hydroxyestrogens from 17beta-estradiol (E2), namely 2- and 4-hydroxy E2 (PubMed:23821647). Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites (By similarity). May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:15258110). Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system (PubMed:15258110). Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2 (By similarity). Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products (By similarity). Plays an important role in retinal vascular development. Under ambient/hyperoxic O2 conditions, promotes angiogenesis and capillary morphogenesis of retinal endothelial cells and pericytes, likely by metabolizing the oxygenated products symptomatic of oxidative stress (PubMed:19005183, PubMed:20032512, PubMed:23568032). Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression (PubMed:23979599).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208663 | Cyp1b1 (tGFP-tagged) - Mouse cytochrome P450, family 1, subfamily b, polypeptide 1 (Cyp1b1) |
CNY 5,040.00 |
|
| MR208663L3 | Lenti ORF clone of Cyp1b1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 1, subfamily b, polypeptide 1 (Cyp1b1) |
CNY 6,460.00 |
|
| MR208663L4 | Lenti ORF clone of Cyp1b1 (mGFP-tagged) - Mouse cytochrome P450, family 1, subfamily b, polypeptide 1 (Cyp1b1) |
CNY 6,460.00 |
