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Ipmk (NM_027184) Mouse Tagged ORF Clone
CAT＃: MR206205
- TrueORF®
Ipmk (Myc-DDK-tagged) - Mouse inositol polyphosphate multikinase (Ipmk)
ORF Plasmid: tGFP
"NM_027184" in other vectors (3)
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CNY 2,850.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2410017C19Rik; AA408208; Impk |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR206205 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGAGCCCCCAGCGCTGCGCCTGCGGCCGCCGGGAAGCACCGGAGACAGCCCGCCGGTGCCGC GCCTGCTCGGAGGCTGCGTGCCGCTGTCGCATCAGGTGGCTGGCCACATGTACGGCAAGGACAAAGTGGG CATACTCCAGCACCCAGATGGTACAGTTCTGAAACAGCTACAGCCACCGCCACGGGGCCCAAGAGAGCTG GAATTTTATACCATGGTTTACGCTGCTGACTGTGCCGATGCTGTTCTCCTGGAGCTGCGAAAACACCTGC CCAAATACTACGGCGTCTGGTCCCCTCCCACCGCACCAAACGATGTGTACCTAAAACTGGAAGATGTGAC TCATAAGTTTAACAAACCCTGTATAATGGACGTGAAGATTGGGCGGAAGAGCTACGACCCCTTTGCGTCA TCAGAGAAGATTCAGCAGCAGGTCAGCAAGTACCCTCTGATGGAGGAGATCGGGTTCCTGGTGCTCGGCA TGAGGGTTTATCATCTTCACTCTGACAGCTACGAGACACAAAACCAGCACTATGGAAGAGGCCTAACGAA AGAGACCCTGAAGGAAGGCGTCTCCAAGTTTTTCCACAATGGCTTCTGTTTAAGAAAAGATGCGATTGCC GCCAGTATTCAGAAGGTAGAGAAGATTCTCCAGTGGTTTGAAAATCAGAAGCAGCTTAACTTTTACGCAA GTTCTTTACTGTTTGTTTATGAAGGTTCATCTCAGCCAGCTACTACAAAAGCAAACGATAGAACTTTGGC GGGGAGGTTTCTCTCCAAAGGACCCCTGACGGATGCGGACGGCCTGGAGTGCAATAACAACTTCCACCTG TTCGGCGCCCCGCCCAACGGGATGTCGGTGGGCAAGAGCTTATCGAAGGCGTACTCGAGGCACAGAAAGC TGTATGCAAAAAAGCACCAGAGTCAGACTTCCCTGAAAGTCGAAACGCTGGAGCAAGACAACGGGTGGAG AAGCATGTCCCAGGAACACTTAAACGGAAACGTGCTTGCCCAACTGGAAAAGGTGTTCTACCACCTTCCC GCGGGTCGTCCGGAGATCCCGGAAGCGGAAGTACGGATGATAGACTTTGCTCACGTGTTCCCTAGCAACA CAGTCGATGAGGGGTATGTTTACGGTCTGAAGCATCTAATTGCCGTGCTTCGGAGTATTTTAGACAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR206205 protein sequence
Red=Cloning site Green=Tags(s) MAAEPPALRLRPPGSTGDSPPVPRLLGGCVPLSHQVAGHMYGKDKVGILQHPDGTVLKQLQPPPRGPREL EFYTMVYAADCADAVLLELRKHLPKYYGVWSPPTAPNDVYLKLEDVTHKFNKPCIMDVKIGRKSYDPFAS SEKIQQQVSKYPLMEEIGFLVLGMRVYHLHSDSYETQNQHYGRGLTKETLKEGVSKFFHNGFCLRKDAIA ASIQKVEKILQWFENQKQLNFYASSLLFVYEGSSQPATTKANDRTLAGRFLSKGPLTDADGLECNNNFHL FGAPPNGMSVGKSLSKAYSRHRKLYAKKHQSQTSLKVETLEQDNGWRSMSQEHLNGNVLAQLEKVFYHLP AGRPEIPEAEVRMIDFAHVFPSNTVDEGYVYGLKHLIAVLRSILDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027184 |
| ORF Size | 1191 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027184.2 |
| RefSeq Size | 5432 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 69718 |
| UniProt ID | Q7TT16 |
| MW | 44.5 kDa |
| Gene Summary | Inositol phosphate kinase with a broad substrate specificity. Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) first to inositol 1,3,4,5-tetrakisphosphate and then to inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) (PubMed:15939867). Phosphorylates inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). Phosphorylates glycero-3-phospho-1D-myo-inositol 4,5-bisphosphate to glycero-3-phospho-1D-myo-inositol 3,4,5-trisphosphate. Plays an important role in MLKL-mediated necroptosis via its role in the biosynthesis of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Binding of these highly phosphorylated inositol phosphates to MLKL mediates the release of an N-terminal auto-inhibitory region, leading to activation of the kinase. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis (By similarity). Required for normal embryonic development, probably via its role in the biosynthesis of inositol 1,3,4,5,6-pentakisphosphate (Ins(1,3,4,5,6)P5) and inositol hexakisphosphate (InsP6) (PubMed:15939867).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
IPMK and β-catenin mediate PLC-β1-dependent signaling in myogenic differentiation
,Ramazzotti, G;Billi, AM;Manzoli, L;Mazzetti, C;Ruggeri, A;Erneux, C;Kim, S;Suh, PG;Cocco, L;Faenza, I;,
Oncotarget
,PubMed ID 27563828
[IPMK]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206205 | Ipmk (tGFP-tagged) - Mouse inositol polyphosphate multikinase (Ipmk) |
CNY 3,140.00 |
|
| MR206205L3 | Lenti ORF clone of Ipmk (Myc-DDK-tagged) - Mouse inositol polyphosphate multikinase (Ipmk) |
CNY 4,750.00 |
|
| MR206205L4 | Lenti ORF clone of Ipmk (mGFP-tagged) - Mouse inositol polyphosphate multikinase (Ipmk) |
CNY 4,750.00 |
