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Ptges2 (NM_133783) Mouse Tagged ORF Clone
CAT＃: MR205997
- TrueORF®
Ptges2 (Myc-DDK-tagged) - Mouse prostaglandin E synthase 2 (Ptges2)
ORF Plasmid: tGFP
"NM_133783" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 0610038H10Rik; C79137; Gbf1; Mpges2; Pges2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR205997 representing NM_133783
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGGCGGCGCGCCTTTCGTGGGTGCTTGTCTCTAGCCGGTGCGCCCTGACTGAGGGGCTGCTCA CACGACCCTGGCAGCCGCTCTCGGCACAGAGCCGGGCTGGCTTCACCAGGGTGGCTGCCGGAAGCCGGGG CGCCGCGGTCCGCAAGGGGAGCCCGCGGTTGCTGGGGGCGGCGGCGCTGGCCCTGGGCGGCGCGCTGGGG CTGTACCACACCGTGCGGTGGCACCAGCGTTCCCAGGACCTCCGCGCGGAGCGCTCGGCTGCGCAGCTGC CCCTGTCGAACAGCCTGCAGCTGACCCTGTACCAGTACAAGACATGTCCCTTCTGCAGCAAAGTCCGTGC CTTCCTCGACTTCCACTCCCTGCCCTATCAGGTGGTGGAGGTGAATCCCGTGAGAAGGACTGAGATCAAA TTCTCCTCCTACAGGAAAGTGCCCATCTTGGTGGCCCAGGAAGGAGACAGCTTGCAACAGCTCAATGACT CCTCTGTCATCATTAGTGCCCTCAAGACCTACCTGGTTTCAGGGCAGCCCCTGGAAGAGGTCATCACTTA TTACCCACCCATGAAGGCCATGAATGACCAGGGCAAGGAGGTGACCGAGTTTTGCAACAAGTACTGGCTC ATGCTGGACGAGAAGGAGGCCCAGCAGATGTATGGCGGGAAGGAAGCCAGGACGGAGGAGATGAAGTGGC GGCAGTGGGCGGATGACTGGCTGGTGCATCTCATCTCTCCCAACGTGTACCGAACACCCGCTGAGGCCTT GGCTTCCTTCGACTACATTGTCCGTGAGGGCAAGTTTGGGGCTGTGGAGGCTGCCATGGCCAAGTATGTG GGCGCGGCTGCCATGTACCTCATCAGCAAGCGCCTCAAAAGCAGGCACCACCTGCAGGATGATGTACGGG TAGACCTCTATGAAGCAGCCAACAAGTGGGTGACAGCCGTGGGTAAAGACCGGCCATTCATGGGGGGTCA GAAGCCTAACCTTGCTGACCTGGCAGTGTATGGTGTGCTGCGAGTGATGGAGGGTCTGGAGGCCTTCGAC GACCTGATGCGACACTCACACATCCAGCCTTGGTACCTGCGGATGGAGCGGGCCATTGAAGAAGCCCCCT CGGTGCATCATGTCAACCCTAGCTGCAAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR205997 representing NM_133783
Red=Cloning site Green=Tags(s) MAQAARLSWVLVSSRCALTEGLLTRPWQPLSAQSRAGFTRVAAGSRGAAVRKGSPRLLGAAALALGGALG LYHTVRWHQRSQDLRAERSAAQLPLSNSLQLTLYQYKTCPFCSKVRAFLDFHSLPYQVVEVNPVRRTEIK FSSYRKVPILVAQEGDSLQQLNDSSVIISALKTYLVSGQPLEEVITYYPPMKAMNDQGKEVTEFCNKYWL MLDEKEAQQMYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPAEALASFDYIVREGKFGAVEAAMAKYV GAAAMYLISKRLKSRHHLQDDVRVDLYEAANKWVTAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLEAFD DLMRHSHIQPWYLRMERAIEEAPSVHHVNPSCKD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133783 |
| ORF Size | 1152 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133783.2, NP_598544.2 |
| RefSeq Size | 1969 bp |
| RefSeq ORF | 1155 bp |
| Locus ID | 96979 |
| UniProt ID | Q8BWM0 |
| MW | 43.8 kDa |
| Gene Summary | The protein encoded by this gene is a Golgi membrane-associated prostaglandin E synthase candidate, which is capable of catalyzing the conversion of prostaglandin H2 to prostaglandin E2 in vitro. However, a study using mice deficient of this gene suggests that this enzyme does not contribute to prostaglandin E2 biosynthesis in vivo. This protein is synthesized as a Golgi membrane-bound protein, but its N-terminal hydrophobic region is cleaved off during protein maturation to produce the predominant soluble truncated form that still retains the enzyme activity. This soluble protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE), possibly via an interaction with CAAAT/enhancer-binding protein-beta. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205660 | Ptges2 (untagged) - Mouse prostaglandin E synthase 2 (Ptges2), (10ug) |
CNY 2,000.00 |
|
| MG205997 | Ptges2 (tGFP-tagged) - Mouse prostaglandin E synthase 2 (Ptges2) |
CNY 3,140.00 |
|
| MR205997L3 | Lenti ORF clone of Ptges2 (Myc-DDK-tagged) - Mouse prostaglandin E synthase 2 (Ptges2) |
CNY 4,750.00 |
|
| MR205997L4 | Lenti ORF clone of Ptges2 (mGFP-tagged) - Mouse prostaglandin E synthase 2 (Ptges2) |
CNY 4,750.00 |
