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Psme3 (NM_011192) Mouse Tagged ORF Clone
CAT＃: MR203255
- TrueORF®
Psme3 (Myc-DDK-tagged) - Mouse proteaseome (prosome, macropain) 28 subunit, 3 (Psme3)
ORF Plasmid: tGFP
"NM_011192" in other vectors (4)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA410043; AU020960; Ki; pa28g; PA28gamma; REGgamma |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR203255 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGTTGCTGAAGGTGGATCAGGAAGTGAAGCTCAAGGTTGATTCTTTCAGAGAGCGGATCACAA GTGAGGCAGAAGACTTGGTGGCAAATTTTTTCCCAAAGAAGTTACTAGAACTTGATAGTTTTTTGAAGGA ACCAATTCTAAATATCCATGACCTAACTCAGATCCACTCAGACATGAACCTCCCAGTCCCTGACCCCATT CTCCTCACCAATAGCCACGATGGACTGGATGGTCCCACTTACAAGAAGCGCAGGTTGGATGAATGTGAAG AGGCCTTTCAAGGAACCAAGGTGTTTGTGATGCCCAATGGGATGTTGAAAAGCAACCAGCAGCTTGTGGA CATTATTGAGAAAGTAAAACCCGAGATTCGGCTGCTGATCGAGAAATGTAACACGGTCAAAATGTGGGTT CAGCTGTTGATTCCCAGAATAGAAGATGGGAACAACTTCGGGGTATCAATTCAGGAGGAAACAGTTGCTG AACTAAGAACTGTGGAGAGTGAAGCCGCATCTTACCTGGACCAGATTTCTAGATATTATATTACAAGAGC CAAATTGGTTTCTAAAATAGCTAAATATCCCCATGTGGAGGACTATCGCCGCACTGTCACAGAGATTGAT GAGAAAGAATACATCAGCCTCCGGCTCATCATCTCAGAGCTGAGGAATCAGTATGTCACTCTCCATGACA TGATCCTGAAAAACATTGAGAAGATCAAACGGCCCCGGAGCAGCAATGCAGAGACACTGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203255 protein sequence
Red=Cloning site Green=Tags(s) MASLLKVDQEVKLKVDSFRERITSEAEDLVANFFPKKLLELDSFLKEPILNIHDLTQIHSDMNLPVPDPI LLTNSHDGLDGPTYKKRRLDECEEAFQGTKVFVMPNGMLKSNQQLVDIIEKVKPEIRLLIEKCNTVKMWV QLLIPRIEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITRAKLVSKIAKYPHVEDYRRTVTEID EKEYISLRLIISELRNQYVTLHDMILKNIEKIKRPRSSNAETLY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011192 |
| ORF Size | 765 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011192.4 |
| RefSeq Size | 2620 bp |
| RefSeq ORF | 765 bp |
| Locus ID | 19192 |
| UniProt ID | P61290 |
| MW | 29.5 kDa |
| Gene Summary | Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209247 | Psme3 (untagged) - Mouse proteaseome (prosome, macropain) 28 subunit, 3 (Psme3), (10ug) |
CNY 3,990.00 |
|
| MG203255 | Psme3 (tGFP-tagged) - Mouse proteaseome (prosome, macropain) 28 subunit, 3 (Psme3) |
CNY 2,850.00 |
|
| MR203255L3 | Lenti ORF clone of Psme3 (Myc-DDK-tagged) - Mouse proteaseome (prosome, macropain) 28 subunit, 3 (Psme3) |
CNY 4,750.00 |
|
| MR203255L4 | Lenti ORF clone of Psme3 (mGFP-tagged) - Mouse proteaseome (prosome, macropain) 28 subunit, 3 (Psme3) |
CNY 4,750.00 |
