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Nf2 (NM_010898) Mouse Tagged ORF Clone
CAT＃: MG226996
- TrueORF®
Nf2 (tGFP-tagged) - Mouse neurofibromatosis 2 (Nf2), (10ug)
ORF Plasmid: DDK
"NM_010898" in other vectors (4)
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CNY 6,480.00
CNY 6,560.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | merlin |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226996 representing NM_010898
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGCCATCGCTTCTCGCATGAGCTTCAGCTCACTCAAGAGGAAGCAGCCCAAGACATTCACGG TGCGGATCGTCACCATGGACGCCGAGATGGAGTTCAACTGCGAGATGAAATGGAAGGGGAAGGACCTGTT TGATTTGGTGTGCCGGACACTGGGGCTTCGGGAAACCTGGTTCTTTGGACTGCAGTATACAATCAAGGAC ACGGTGGCCTGGCTCAAAATGGACAAGAAGGTGTTGGATCATGATGTTTCGAAGGAAGAACCAGTTACCT TTCACTTCCTGGCCAAATTTTATCCTGAAAATGCTGAGGAGGAGCTAGTTCAAGAGATCACGCAACACTT ATTTTTCTTACAGGTGAAGAAGCAGATTTTGGATGAAAAGGTCTACTGCCCTCCCGAGGCGTCCGTGCTC TTGGCGTCATATGCTGTCCAGGCCAAGTATGGCGACTATGACCCCTCTGTGCACAAGCGGGGATTTTTAG CCCAAGAGGAATTGCTCCCGAAAAGGGTGATAAATCTCTATCAGATGACTCCGGAAATGTGGGAGGAGAG AATTACGGCTTGGTATGCGGAGCACCGGGGCAGAGCCAGGGATGAAGCTGAAATGGAGTATTTGAAGATA GCTCAGGACCTGGAGATGTATGGTGTGAACTACTTTACAATCCGGAATAAAAAGGGCACAGAGTTGCTGC TTGGAGTGGATGCTCTTGGGCTTCATATCTATGACCCTGAGAACAGGCTGACCCCCAAGATCTCCTTCCC ATGGAATGAAATCCGAAACATCTCCTACAGCGACAAGGAGTTTACTATTAAACCACTGGATAAGAAAATT GATGTCTTCAAATTTAACTCCTCAAAGCTTCGTGTTAATAAGCTGATTCTTCAGCTATGTATTGGGAACC ATGACCTATTTATGAGGCGACGGAAAGCTGACTCTTTAGAAGTTCAGCAGATGAAAGCCCAGGCCAGGGA AGAGAAGGCTAGAAAGCAGATGGAAAGGCAGCGGCTGGCTCGAGAGAAGCAGATGCGGGAGGAGGCCGAG CGTACAAGAGATGAGTTAGAGAGGAGGCTCCTGCAGATGAAAGAAGAAGCAACGATGGCCAATGAAGCTC TGATGCGCTCTGAGGAGACAGCTGATCTGTTGGCTGAAAAGGCTCAGATCACAGAGGAGGAGGCCAAGCT TTTGGCACAAAAGGCTGCAGAGGCTGAGCAAGAGATGCAGCGAATCAAGGCCACGGCCATTCGGACAGAG GAGGAGAAGCGCCTGATGGAGCAGAAGGTGCTGGAGGCTGAAGTGCTGGCATTGAAGATGGCTGAGGAGT CAGAGAGGAGGGCCAAGGAGGCTGATCAGTTAAAGCAAGACTTGCAAGAAGCCAGAGAAGCAGAGCGAAG AGCCAAGCAGAAGCTCTTAGAAATCGCCACCAAGCCCACCTATCCACCCATGAACCCAATTCCACCACCA CTGCCTCCTGACATACCGAGCTTCGACATTATTGCTGACAGCTTGTCATTTGACTTCAAGGATACGGACA TGAAGCGACTTTCCATGGAGATAGAGAAAGAAAAAGTGGAGTACATGGAGAAGAGCAAGCACCTGCAGGA GCAGCTCAACGAGCTCAAGACGGAGATCGAGGCCTTGAAACTCAAAGAGCGGGAGACGGCCTTGGACGTC CTACACAGCGAGAGCTCAGACAGAGGCGGCCCCAGCAGCAAGCATAATACCATTAAAAAGCTCACTCTGC AGAGCGCCAAGTCCCGAGTGGCCTTCTTTGAAGAACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226996 representing NM_010898
Red=Cloning site Green=Tags(s) MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKD TVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVL LASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKI AQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKI DVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAE RTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTE EEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPPP LPPDIPSFDIIADSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDV LHSESSDRGGPSSKHNTIKKLTLQSAKSRVAFFEEL TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010898 |
| ORF Size | 1788 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010898.4, NP_035028.2 |
| RefSeq Size | 4708 bp |
| RefSeq ORF | 1791 bp |
| Locus ID | 18016 |
| UniProt ID | P46662 |
| Gene Summary | Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex (By similarity). Plays a role in lens development and is required for complete fiber cell terminal differentiation, maintenance of cell polarity and separation of the lens vesicle from the corneal epithelium.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219508 | Nf2 (untagged) - Mouse neurofibromatosis 2 (Nf2), (10ug) |
CNY 7,700.00 |
|
| MR226996 | Nf2 (Myc-DDK-tagged) - Mouse neurofibromatosis 2 (Nf2) |
CNY 5,990.00 |
|
| MR226996L3 | Lenti ORF clone of Nf2 (Myc-DDK-tagged) - Mouse neurofibromatosis 2 (Nf2) |
CNY 7,890.00 |
|
| MR226996L4 | Lenti ORF clone of Nf2 (mGFP-tagged) - Mouse neurofibromatosis 2 (Nf2) |
CNY 7,890.00 |
