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Blm (NM_007550) Mouse Tagged ORF Clone
CAT＃: MG226502
- TrueORF®
Blm (tGFP-tagged) - Mouse Bloom syndrome RecQ helicase-like (Blm) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_007550" in other vectors (4)
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CNY 17,136.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226502 representing NM_007550
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGATCATGGCTGCTGTTCCTCTGAACAATCTACAAGAACAACTACAGCGACACTCAGCCAGAAAAC TTAATAATCAACCCAGCCTTTCAAAACCAAAATCTTTAGGTTTTACTTTTAAAAAGAAAACATCAGAGGG TGATGTGTCTGTCACTAGTGTGTCCGTAGTAAAAACACCTGCGTTAAGTGATAAAGATGTGAACGTGTCT GAGGCCTTTTCATTCACTGAGTCTCCACTCCACAAACCAAAGCAGCAGGCAAAGATTGAAGGCTTCTTTA AACATTTCCCTGGAAGGCAGCAAAGCAAGGGGACCTGCTCTGAGCCGTCACTGCCGGCCACGGTACAGAC TGCTCAGGACACTTTGTGCACTACCCCCAAAACCCCCACTGCGAAGAAACTGCCCGTGGCTGTTTTCAAG AAATTAGAATTTAGTTCTTCTGCAGACTCCCTCAGTGACTGGGCTGATATGGATGACTTTGATATGTCAG CATCAGATGCGTTTGCTTCACTGGCTAAAAATCCTGCCACAAGAGTAAGCACCGCTCAGAAAATGAAAAA GACTAAGAGAAACTTCTTTAAACCACCACCTCGTAAAGCCAATGCAGTAAAGACTGACTTGACTCCTCCC TCCCCCGAATGCCTGCAAGTGGATTTAACGAAGGAATCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCGG AGGGGGCGGACTGCCTGAGCAGGGATGTGATCTGCATTGACAATGATTCTGCTTCTGAAGAGCTCACGGA GAAAGACACGCAGGAAAGCCAGTCTTTGAAAGCTCACTTGGGAGCTGAAAGAGGTGACAGTGAAAAGAAG AGCCATGAAGACGAAGCTGTGTTCCATTCAGTTCAGAACACTGAATACTTTGAACACAATGACAATGATT ATGATATAGATTTTGTTCCACCTTCTCCAGAAGAAATCATCTCCACTGCTTCTTCCTCGTTGAAATGTTC CAGTATGTTAAAGGATCTTGATGACTCTGACAAAGAAAAGGGCATTCTTAGCACCTCAGAAGAGCTTCTG TCAAAACCAGAGGAAATGACCACACACAAGTCTGATGCAGGAACCAGTAAAGACTGTGATGCCCAGCAGA TACGCATACAGCAGCAGCTTATTCATGTGATGGAGCACATCTGTAAGTTAGTTGATACTGTTCCTACTGA TGAACTGGAAGCTTTGAATTGTGGGACCGAATTGCTTCAACAACGAAACATAAGGAGGAAGCTCCTAGCT GAAGCAGGTTTTAATGGAAATGACGTCAGACTTCTGGGTTCTCTGTGGAGGCACAGGCCTGATTCACTTG ATAACACAGTGCAGGGCGACTCCTGCCCTGTGGGGCATCCTAATAAAGAGTTAAATTCTCCATACCTTCT CTCACATTCCCCTTCCACTGAGGAATGTTTACCCACCACCACTCCAGGAAAGACAGGATTCTCAGCCACC CCGAAGAATCTCTTTGAAAGGCCGTTATTGAATTCCCATTTACAGAAGTCCTTTGTAAGTAGCAACTGGG CTGAAACACCAAGGATGGAAAACAGGAACGAAAGCACTGACTTCCCAGGGAGTGTTCTCACCAGCACCAC TGTGAAAGCTCAGAGTAAACAAGCTGCTTCAGGATGGAACGTAGAGAGACACGGCCAGGCTTCCTATGAT ATCGATAACTTTAATATTGATGACTTTGATGATGATGATGATGATGATGACTGGGAAAACATAATGCACA ATTTTCCAGCCAGCAAATCTTCCACAGCCACCTACCCACCCATCAAGGAAGGTGGGCCAGTTAAATCTCT CTCAGAAAGGATTTCTTCAGCCAAGGCAAAGTTTCTTCCAGTGGTATCAACCGCTCAAAATACAAACCTC TCAGAGTCAATTCAGAATTGCTCTGATAAGCTGGCCCAAAATTTATCATCAAAAAATCCAAAACATGAAC ATTTTCAAAGTCTTAATTTTCCTCATACAAAAGAAATGATGAAGATTTTCCATAAGAAATTTGGCTTGCA TAATTTTAGAACTAATCAGCTAGAGGCGATCAATGCTGCGCTGCTTGGTGAAGACTGCTTTATCCTAATG CCCACTGGAGGAGGTAAAAGTTTGTGCTACCAGCTCCCTGCCTGTGTTTCTCCTGGGGTCACAATTGTCA TTTCTCCCTTGAGATCACTAATAGTAGATCAAGTCCAAAAGCTGACTTCCTTTGATATTCCAGCTACATA TCTGACAGGGGATAAGACTGACTCAGAAGCTGCAAATATTTACCTCCAATTATCCAAAAAAGACCCAATC ATCAAGCTTTTGTATGTTACTCCAGAGAAGGTCTGTGCAAGTAACAGGCTGATTTCTACTCTGGAGAATC TGTATGAGCGGAAGCTCTTGGCACGTTTTGTCATTGATGAAGCGCATTGTGTGAGTCAGTGGGGTCATGA TTTTCGTCAAGATTACAAAAGGATGAATATGCTTCGCCAGAAGTTTCCTTCTGTTCCAGTGATGGCCCTC ACGGCCACAGCGAACCCCAGGGTCCAGAAGGACATCCTCACTCAGCTGAAGATCCTCAGACCTCAGGTGT TTAGCATGAGCTTTAACAGACACAATCTGAAGTACTATGTATTACCCAAGAAGCCCAAAAAAGTAGCATT TGATTGCCTAGAGTGGATCAGAAAGCATCACCCTTATGACTCGGGGATAATTTACTGCCTCTCCAGGAGG GAATGTGACACAATGGCTGACACTTTACAGAGAGAAGGCCTGGCTGCCCTGGCTTACCATGCGGGCCTCA GTGACTCTGCCAGAGATGAGGTGCAGCACAAGTGGATCAACCAGGACAACTGCCAGGTTATCTGTGCGAC AATTGCGTTTGGAATGGGAATTGACAAACCTGACGTGCGATTTGTGATTCATGCATCTCTTCCTAAATCT ATGGAGGGTTATTATCAAGAATCCGGCCGAGCTGGAAGAGATGGGGAAATATCTCACTGCGTGCTTTTCT ATACATATCATGATGTGACCAGACTGAAGAGACTTATAATGATGGAAAAAGATGGAAACTATCATACGAA GGAAACTCACGTCAATAATCTATATAGCATGGTACATTACTGTGAAAACATAACGGAATGCAGAAGAATA CAGCTTTTAGCTTACTTTGGTGAAAAAGGATTCAACCCTGATTTTTGTAAGAAATACCCAGATGTTTCTT GTGACAATTGCTGTAAAACAAAGGATTATAAAACAAAAGATGTGACTGATGACGTGAAAAATATTATAAG ATTTGTTCAAGAACACAGTTCATCACCAGGAACAAGAAATATAGGACCTGCTGGAAGATTTACTCTGAAC ATGCTGGTCGACATTTTCTTGGGGAGCAAGAGTGCAAAAGTTAAGTCTGGAATATTTGGAAAGGGGACTA CATATTCACGACATAATGCCGAAAGACTTTTTAAAAAGCTGATTCTAGACAAAATCCTGGATGAAGACTT ATATATCAATGCCAATGACCAACCAATTGCCTATGTGATGCTAGGAACAAAAGCCCACAGTGTGCTGAGT GGCCACTTGAAGGTGGACTTCATGGAAACGGAAAATTCCAGCAGTATTAAAAAACAAAAAGCTTTAGTGG CCAAAGTATCCCAGAGAGAAGAGGTAGTTAAGAAATGTCTTGGAGAACTTACAGAGGTCTGCAAATTGCT GGGGAAAGTCTTTGGTGTCCATTACTTCAATATTTTTAATACAGCCACACTCAAAAAGCTTGCAGAATCT TTATCTTCTGATCCTGAGGTTTTGCTTCAGATTGATGGTGTTACCGAAGACAAGCTGGAAAAATATGGTG CAGAAGTGATTCCAGTATTACAGAAGTACTCAGAATGGACAGTGCCAGCTGAGGATGGTTCCCCAGGCGC CAGAGGCGCCCCAGAGGACACTGAGGAGGAGGAGGAGGAAGCGCCTGTATCTTCTCACTACTTTGCAAAT CAAACTAGAAATGAAAGAAAGAGAAAGAAAATGTCAGCCACCCATAAGCCCAAGAGGAGAAGAACTAGTT ACGGTGGCTTCAGAGCAAAGGGGGGCTCTACTACATGCAGAAAAACGACTTCTAAAAGTAAATTCTATGG TGTAACTGGATCCCGCTCGGCCTCATGTGCTTCTCAGGCAACATCATCAGCCAGTAGAAAACTGGGGATT ATGGCTCCTCCAAAGCCTGTAAATAGAACGTTCCTCAGGCCTTCATATGCCTTCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226502 representing NM_007550
Red=Cloning site Green=Tags(s) MRIMAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVS EAFSFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFK KLEFSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPP SPECLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKK SHEDEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELL SKPEEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLA EAGFNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSAT PKNLFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYD IDNFNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNL SESIQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILM PTGGGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPI IKLLYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMAL TATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRR ECDTMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVIHASLPKS MEGYYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTKETHVNNLYSMVHYCENITECRRI QLLAYFGEKGFNPDFCKKYPDVSCDNCCKTKDYKTKDVTDDVKNIIRFVQEHSSSPGTRNIGPAGRFTLN MLVDIFLGSKSAKVKSGIFGKGTTYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLS GHLKVDFMETENSSSIKKQKALVAKVSQREEVVKKCLGELTEVCKLLGKVFGVHYFNIFNTATLKKLAES LSSDPEVLLQIDGVTEDKLEKYGAEVIPVLQKYSEWTVPAEDGSPGARGAPEDTEEEEEEAPVSSHYFAN QTRNERKRKKMSATHKPKRRRTSYGGFRAKGGSTTCRKTTSKSKFYGVTGSRSASCASQATSSASRKLGI MAPPKPVNRTFLRPSYAFS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007550 |
| ORF Size | 4257 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007550.4, NP_031576.4 |
| RefSeq Size | 4869 bp |
| RefSeq ORF | 4260 bp |
| Locus ID | 12144 |
| UniProt ID | O88700 |
| Gene Summary | ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9840919). Participates in DNA replication and repair (By similarity). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:9840919). Negatively regulates sister chromatid exchange (SCE) (PubMed:9840919, PubMed:27010503). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224422 | Blm (untagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 1, (10ug) |
CNY 18,240.00 |
|
| MR226502 | Blm (Myc-DDK-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 1 |
CNY 14,160.00 |
|
| MR226502L3 | Lenti ORF clone of Blm (Myc-DDK-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 1 |
CNY 16,060.00 |
|
| MR226502L4 | Lenti ORF clone of Blm (mGFP-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 1 |
CNY 16,060.00 |
