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Eif4a1 (NM_001159375) Mouse Tagged ORF Clone
CAT＃: MG226454
- TrueORF®
Eif4a1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001159375" in other vectors (3)
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CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BM-010; Ddx2a; Eif4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226454 representing NM_001159375
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCGAGTCAGGATTCTCGATCCAGAGACAATGGCCCCGACGGGATGGAGCCGGAAGGCGTCATCG AGAGTAACTGGAACGAGATTGTGGATAGCTTTGATGACATGAATCTCTCAGAGTCCCTCCTCCGTGGTAT TTATGCCTATGGTTTTGAGAAGCCCTCTGCCATCCAGCAGCGAGCTATTCTTCCTTGTATCAAGGGTTAT GATGTGATTGCTCAAGCCCAGTCTGGGACTGGGAAAACAGCTACATTTGCCATATCAATTCTGCAGCAGA TTGAATTAGATCTAAAGGCCACTCAGGCTTTGGTTCTGGCACCCACACGTGAATTGGCTCAGCAGATACA AAAGGTGGTTATGGCATTAGGAGACTACATGGGTGCCTCTTGTCATGCCTGCATTGGGGGCACCAATGTG CGTGCTGAGGTGCAGAAGCTGCAGATGGAAGCTCCCCATATCATCGTGGGTACCCCTGGCCGGGTGTTTG ACATGCTTAACCGGAGATACCTGTCCCCCAAATACATCAAGATGTTCGTACTGGATGAAGCAGATGAAAT GTTAAGCCGAGGGTTCAAGGATCAGATCTATGACATATTCCAGAAGCTCAACAGCAACACACAGGTAGTT TTGTTGTCTGCTACAATGCCTTCTGATGTCCTTGAGGTGACCAAGAAATTTATGAGAGACCCTATTCGGA TTCTTGTCAAGAAGGAAGAATTGACCCTGGAGGGTATCCGCCAATTCTACATCAATGTGGAACGAGAGGA GTGGAAGCTTGACACATTGTGTGACTTGTATGAGACGCTGACCATCACCCAGGCAGTCATCTTTATCAAC ACCAGAAGGAAGGTGGACTGGCTCACCGAGAAGATGCATGCCCGAGATTTCACTGTTTCTGCCATGCACG GAGATATGGACCAAAAGGAACGAGATGTGATCATGAGGGAGTTCCGGTCTGGCTCTAGCAGAGTATTAAT TACCACTGACCTGTTGGCCAGAGGCATTGATGTGCAGCAGGTCTCCTTAGTCATCAACTATGACCTTCCC ACCAACAGGGAAAACTACATCCACAGGTGGTCGGTTTGGTCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226454 representing NM_001159375
Red=Cloning site Green=Tags(s) MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGY DVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNV RAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVV LLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFIN TRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLP TNRENYIHRWSVWS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001159375 |
| ORF Size | 1092 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001159375.1, NP_001152847.1 |
| RefSeq Size | 1886 bp |
| RefSeq ORF | 1095 bp |
| Locus ID | 13681 |
| Gene Summary | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR226454 | Eif4a1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1), transcript variant 2 |
CNY 3,610.00 |
|
| MR226454L3 | Lenti ORF clone of Eif4a1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1), transcript variant 2 |
CNY 5,510.00 |
|
| MR226454L4 | Lenti ORF clone of Eif4a1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1), transcript variant 2 |
CNY 5,510.00 |
