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Bmx (NM_009759) Mouse Tagged ORF Clone
CAT＃: MG226349
- TrueORF®
Bmx (tGFP-tagged) - Mouse BMX non-receptor tyrosine kinase (Bmx), (10ug)
ORF Plasmid: DDK
"NM_009759" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Etk; Etk/Bmx; Tyro8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226349 representing NM_009759
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACAATATGGAGAGTAAATCCATTCTGGAAGAACTGCTTCTCAAAAAGTCACAGCAAAAGAAGA AAATGTCACCAAATAATTACAAGGAGCGGCTTTTTGTTCTAACCAAAACAAGCCTTTCCTACTATGAATA CGACAAAATGAAAAGAGGAAGCAGAAAAGGATCAATAGAAATTAAGAAAATCAGATGTGTGGAAAAAGTC AACCTTGAGGAGCAGACACCGGTGGAGAGACAGTACCCATTTCAGATTGTGTATAAAGATGGGCTTCTTT ATGTCTATGCATCGAATGAAGAGAGCCGATGTCAGTGGTTAAAAGCGCTGCAAAAAGAGATACGGGGCAA CCCTCACCTGTTGATCAAGTATCACAGTGGCTTCTTTGTGGATGGAAAGTTCCTGTGTTGCCAGCAGAGC TGCAAAGCAGCCCCAGGATGCACTCTCTGGGAAGCATATGCTGATCTGCACATCGCAATCAGTGATGAGA AACACAGAGCTCCCACTTTCCCAGAGAGGCTACTGAAGATTCCAAGGGCAGTTCCCGTTCTCAAAATGGA TGCATCATCTTCAGGTGCCATTCTACCCCAATATGACAGCTATTCAAAGAAAAGTTGTGGTTCCCAGCCA ACCAGCAACATACGCTATATTCCAAGGGAAGACTGCCCTGACTGGTGGCAAGTAAGAAAACTGAAAAGCG AGGAAGACATTGCATGCAGTAACCAACTGGAAAGAAATATCGCCTCTCACAGCACCTCAAAGATGTCATG GGGATTCCCTGAGTCAAGTTCATCAGAAGAAGAGGAAAATCTGCATGCTTATGACTGGTTTGCTGGGAAT ATCTCCAGGTCACAATCTGAGCAGTTACTGAGACAAAAGGGAAAAGAAGGAGCATTTATGGTTCGGAATT CCAGCCAGATGGGAATGTACACTGTGTCCTTATTTAGTAAGGCTGTAAATGATAAAAAAGGAACTGTCAA GCATTACCACGTGCATACTAATGCTGAAAATAAATTATACCTGGCTGAAAACTACTGCTTTGATTCCATT CCAAAGCTCATTCACTATCACCAACATAATTCAGCAGGCATGATCACACGGCTCCGCCACCCAGTGTCAA CCAAGGCCAACAAGGTCCCGGTGTCTGTGGCTCTGGGAAGTGGAATTTGGGAACTGAAAAGAGAAGAGAT TACCTTGCTGAAGGAGCTGGGCAATGGCCAGTTTGGAGTGGTCCAGCTGGGCCAGTGGAAGGGGCAATAT GATGTGGCTGTAAAGATGATCAAGGAGGGTGCCATGTCAGAAGATGAATTCTTTCAGGAGGCCCAGACCA TGATGAAACTCAGCCATCCCAAGCTGGTGAAGTTCTACGGAGTATGCTCAAAGAAATACCCCATCTATAT AGTAACTGAGTATATAACAAATGGTTGCTTGCTTAATTACCTGAAGAGTCATGGGAAAGGACTAGAAAGT TGCCAGCTCTTAGAAATGTGTTATGATGTCTGTGAAGGCATGGCCTTCTTGGAGAGCCATCAGTTCATAC ATCGAGATTTGGCTGCTCGGAACTGTTTGGTGGACAGTGATCTCTCTGTGAAAGTCTCAGACTTTGGAAT GACGAGATATGTCCTTGATGATCAGTATGTCAGCTCAGTAGGAACCAAGTTTCCAGTCAAGTGGTCAGCT CCAGAGGTGTTTCACTACTTCAAATACAGCAGCAAGTCGGATGTATGGGCATTTGGGATCCTGATGTGGG AGGTGTTTAGTCTAGGGAAGCAGCCCTATGACTTATATGATAACTCCGAGGTGGTTGTGAAGGTCTCCCA GGGCCACAGACTCTACCGGCCCCAACTGGCATCAGACACCATCTACCAGATCATGTACAGCTGCTGGCAT GAGCTTCCAGAAAAGCGCCCCACATTTCAGCAACTCCTGTCTGCCATAGAACCACTTCGGGAACAAGATA AACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226349 representing NM_009759
Red=Cloning site Green=Tags(s) MDDNMESKSILEELLLKKSQQKKKMSPNNYKERLFVLTKTSLSYYEYDKMKRGSRKGSIEIKKIRCVEKV NLEEQTPVERQYPFQIVYKDGLLYVYASNEESRCQWLKALQKEIRGNPHLLIKYHSGFFVDGKFLCCQQS CKAAPGCTLWEAYADLHIAISDEKHRAPTFPERLLKIPRAVPVLKMDASSSGAILPQYDSYSKKSCGSQP TSNIRYIPREDCPDWWQVRKLKSEEDIACSNQLERNIASHSTSKMSWGFPESSSSEEEENLHAYDWFAGN ISRSQSEQLLRQKGKEGAFMVRNSSQMGMYTVSLFSKAVNDKKGTVKHYHVHTNAENKLYLAENYCFDSI PKLIHYHQHNSAGMITRLRHPVSTKANKVPVSVALGSGIWELKREEITLLKELGNGQFGVVQLGQWKGQY DVAVKMIKEGAMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKKYPIYIVTEYITNGCLLNYLKSHGKGLES CQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDSDLSVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSA PEVFHYFKYSSKSDVWAFGILMWEVFSLGKQPYDLYDNSEVVVKVSQGHRLYRPQLASDTIYQIMYSCWH ELPEKRPTFQQLLSAIEPLREQDKP TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009759 |
| ORF Size | 1965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009759.4, NP_033889.2 |
| RefSeq Size | 3005 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 12169 |
| UniProt ID | P97504 |
| Gene Summary | Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220149 | Bmx (untagged) - Mouse BMX non-receptor tyrosine kinase (Bmx), (10ug) |
CNY 8,460.00 |
|
| MR226349 | Bmx (Myc-DDK-tagged) - Mouse BMX non-receptor tyrosine kinase (Bmx) |
CNY 4,560.00 |
|
| MR226349L3 | Lenti ORF clone of Bmx (Myc-DDK-tagged) - Mouse BMX non-receptor tyrosine kinase (Bmx) |
CNY 6,460.00 |
|
| MR226349L4 | Lenti ORF clone of Bmx (mGFP-tagged) - Mouse BMX non-receptor tyrosine kinase (Bmx) |
CNY 6,460.00 |
