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Mapk10 (NM_009158) Mouse Tagged ORF Clone
CAT＃: MG226312
- TrueORF®
Mapk10 (tGFP-tagged) - Mouse mitogen-activated protein kinase 10 (Mapk10) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_009158" in other vectors (5)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C230008H04Rik; JNK; JNK3; JNK3B1; JNK3B2; p54bSAPK; p493F1; p493F12; SAPK(beta); Ser; Serk2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226312 representing NM_009158
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTCCATTTCTTATACTACTGCAGTGAACCAACCTTGGATGTGAAAATTGCCTTTTGTCAGGGAT TCGATAAACACGTGGATGTGTCATCTATTGCCAAACATTACAACATGAGCAAAAGCAAGGTGGACAACCA GTTCTACAGTGTGGAAGTGGGGGACTCAACCTTCACCGTTCTTAAGCGCTACCAGAACCTGAAGCCAATT GGCTCTGGGGCTCAGGGAATAGTCTGTGCTGCGTACGACGCTGTCCTTGACAGAAATGTGGCCATTAAGA AGCTCAGCAGACCCTTCCAGAACCAAACTCACGCCAAGAGGGCTTACCGGGAGCTGGTCCTCATGAAGTG TGTGAACCATAAAAACATTATTAGCTTATTAAATGTTTTTACACCCCAGAAAACACTGGAGGAGTTCCAA GATGTCTACTTAGTGATGGAACTGATGGACGCCAACCTGTGTCAGGTGATTCAGATGGAGCTGGACCACG AGCGGATGTCTTACTTGCTGTACCAGATGCTGTGTGGCATCAAGCACCTCCACTCCGCTGGGATCATCCA CAGGGACTTAAAACCCAGTAACATTGTAGTCAAGTCTGATTGCACACTGAAAATCCTCGACTTCGGACTG GCCAGGACAGCGGGTACAAGCTTCATGATGACTCCGTATGTGGTGACGCGATATTACAGAGCCCCTGAGG TCATCCTGGGCATGGGCTACAAGGAGAACGTGGACATATGGTCTGTGGGATGCATCATGGGAGAAATGGT TCGCCACAAAATCCTCTTTCCCGGAAGGGACTATATTGACCAGTGGAACAAAGTCATCGAGCAGCTAGGA ACTCCGTGTCCAGAGTTCATGAAGAAATTGCAGCCCACAGTCAGAAACTACGTGGAGAATCGGCCCAAGT ACGCAGGACTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTCCCAGCGGATTCTGAGCACAATAAACT TAAAGCCAGCCAAGCCAGGGATTTGTTGTCTAAGATGTTAGTGATTGACCCAGCGAAGAGGATATCGGTG GACGACGCACTGCAGCATCCGTACATCAACGTTTGGTACGACCCGGCTGAAGTGGAGGCGCCTCCGCCTC AGATATATGATAAGCAGCTGGATGAAAGGGAGCACACCATCGAAGAATGGAAAGAACTTATCTACAAGGA GGTAATGAACTCAGAAGAGAAGACTAAGAATGGCGTAGTCAAAGGCCAGCCCTCGCCTTCAGCACAGGTG CAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226312 representing NM_009158
Red=Cloning site Green=Tags(s) MSLHFLYYCSEPTLDVKIAFCQGFDKHVDVSSIAKHYNMSKSKVDNQFYSVEVGDSTFTVLKRYQNLKPI GSGAQGIVCAAYDAVLDRNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIISLLNVFTPQKTLEEFQ DVYLVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGL ARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLG TPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISV DDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKELIYKEVMNSEEKTKNGVVKGQPSPSAQV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009158 |
| ORF Size | 1266 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009158.3, NP_033184.2 |
| RefSeq Size | 7203 bp |
| RefSeq ORF | 1269 bp |
| Locus ID | 26414 |
| Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209721 | Mapk10 (untagged) - Mouse mitogen-activated protein kinase 10 (Mapk10), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG206714 | Mapk10 (tGFP-tagged) - Mouse mitogen activated protein kinase 10 (Mapk10), transcript variant 1 |
CNY 5,130.00 |
|
| MR226312 | Mapk10 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 10 (Mapk10), transcript variant 1 |
CNY 5,488.00 |
|
| MR226312L3 | Lenti ORF clone of Mapk10 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 10 (Mapk10), transcript variant 1 |
CNY 5,230.00 |
|
| MR226312L4 | Lenti ORF clone of Mapk10 (mGFP-tagged) - Mouse mitogen-activated protein kinase 10 (Mapk10), transcript variant 1 |
CNY 5,230.00 |
