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Lrrk1 (NM_146191) Mouse Tagged ORF Clone
CAT＃: MG225310
- TrueORF®
Lrrk1 (tGFP-tagged) - Mouse leucine-rich repeat kinase 1 (Lrrk1), (10ug)
ORF Plasmid: DDK
"NM_146191" in other vectors (2)
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CNY 17,860.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW319595; C230002E15Rik; D130026O16Rik; mKIAA1790 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225310 representing NM_146191
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGCACGTCACAGCGGCCACCCAGTATGTACTGGTGTGTGGGGACAGAAGGATTAGCTGTGTGTC CAGGACCTGCCATGGAGACCCATAATGGTGCCGAAGACATGGGCAGCAAACTGTCTTTACCAGGTGGCAG CTCCACGGTGCAGTGCCCCAGCATGGAAGAAATCCACACTGCGTACAAGCAGAGGAACCTCTCCCGAGCC CGAGACCTACTCAGAGGGGTTTGTGAAGAGAGCGAATCCTCACAGGAGAAGGGCCAGCTGCTGAGCATCG CGGCAGCCCACGGGGATCTGGAGACTGTTCAGTTCCTGCTCACTGAGAAGCGCGTGGAGCTGCCGACGGA GCCCACCGATGACAACCCAGCTGTGGTGGCAGCGCATTTTGGACACGCCGAAGTAGTACGTGAACTACTA GAGTCCTTGCCAGGTCCCTGTACCCCCCAGCGGCTACTGAACTGGATGCTGGCCCTGGCTTGCCAGCGAG GGCACCTGGAGGTTGTGAAGCTGCTGGTCCTGACACATGGGGCTGACCCGGAGAACTATGCCGTCAGGAA GAATGAGTTCCCCGTCATTGTGCGGTTGCCCCTCTATGCGGCCATCAAGGCAGGGAATGAAGACATTGCC ATATTCCTGCTTCGGCACGGAGCCTATTTCTGCTCCTACATCCTGCTGGATAGTCCTGAGCCAAGCAAAC ATCTGCTCAGGAAGTATTTCATCGAAGCCAGCGCCCTGCCCAGCAGCTATCCCGGGAAAATCGCACTTCG TGTGAAATGGTCCCATCTGAAGTTGCCCTGGGTGGACCTGGACTGGCTCCTAGACATCTCCTGCCAGATC ACAGAGCTTGACCTGTCTGCCAACTGCCTGCCTTCGCTCCCTTCCATCATCCCTTGGGGACTCATCAATC TAAAGAAGCTGAATCTCTCAAACAACCAGCTGGGGGAGCTGCCCTGCGTGCAGTCCTCCGACGAAATCAT CTGCTCCAGGTTACTTGAAATTGACATCTCCAGCAACAAACTGTCTCACCTCCCTCCTGGATTCCTGCAC CTCTCAAAACTTCAAAAGCTGACTGCTTCCAAAAACTACCTGGAGCGGCTGTTTGAAGAAGAAAACGCCA CTAACTGGATCGGCCTGCGGAAGCTGCAAGAACTCGATCTAGCTGACAACAGGCTGACAGAGCTCCCTGT CCAATTTATGCACTCCTTCAAGTCTCTCACCTCTCTGAATGTCTCCAGGAACAACCTGAAGAGCTTCCCG GATCCCTGGTCCTGCCCTTTGAAATGTTGTAAAGCCTCCAAAAATGCCCTGGAATCGCTGCCAGACAAAA TGGCTGTCTTTTGGAAAAGCCACCTTCGGGATGCAGATTTCTCTGAGAACTCTCTGAAAGAGGTGCCCCT GGGATTGTTCCAGCTTGACGCCCTCATGTTCTTGAGGTTACAGGGAAACCAGCTGTTGTCGCTGCCACCT CAAGAGAAGTGGACCTGCACCCAACTCAAGACCCTGGACCTCTCCAGAAACCAGCTCGGCAAAAATGAAG ATGGGCTTAAAACAAAGAGGATCTCCCTGTTCACCACCAGAGGGCGCCAGCGTTCTGGAACTGAGACAGC CTCCATGTTGGAATTCCCAGCTTTCCTAAGTGAGTCTTTGGAGGTCCTTTGTCTGAATGACAACCATCTT GATGCTGTTCCCCCATCGGTCTGCCTGCTGAAGAACCTCTCAGAGCTCTACTTGGGCAATAACCCTGGCC TCCGAGAGCTCCCTCCGGAGCTAGGCCAGCTGGGGAACCTCTGGCAACTGGACATTGAAGATCTGAACAT TAGCAACGTGCCTGCGGAGGTCAGGAAAGAAGGCCCGAAAGCAACACTGTCTTTCCTGCGTGCTCAGCTG CGCAAAGCAGAGAAGTGTAAGCTGATGAAGATGATCCTCGTGGGTCCCCCGCGCCAGGGCAAGTCCACAC TCCTGGAGATCTTACAGACCGGGAAGGCCCCGCAGCTAGCACACAGTGAAGCCACCATCAGGACCACCAA GTGGGAGCTCCAGAGACCAGCAGGCTCCAAAGCCAAGGTTGAGTCCGTAGAGTTCAACGTCTGGGACATC GGGGGTCCGGCCAGCATGGCCACGGTCAATCAGTGCTTCTTCACAGACAAGGCGCTGTATGTGGTGGTCT GGAACCTGGCACTGGGAGAGGAAGCCGTGGCCAACCTCCAGTTCTGGCTGCTCAACATTGAGGCCAAGGC CCCAAATGCCGTTGTGCTGGTGGTCGGGACACACCTGGACCTAATCGAAGCTAAGTTCCGAGTGGAGAGG ATAGCAACCCTGCGTGCCTACGTGCTGGCCCTCTGCCGCTCGCCATCGGGCTCCAGGGCTACGGGCTTCC CGGACATCACTTTCAAACACTTGCATGAGATCTCCTGCAAGAATCTGGAAGGGCAGGAGGGGCTGCGGCA ACTGATCTTCCACGTCACGTGCAACATGAAGGATGTGGGTAGCACTATCGGCTGTCAAAAACTCGCCGGC AGGCTGATCCCCAGGAGCTACATAAGCCTACAGGAGGCCGTGCTGGCTGAGCAACAGCGCCGCAGTCTGG GTGACCAAGTACAGTATCTGACGGACAGGCAGCTGGACCAGCTGGTAGAGCAGACGCCTGGCAATGATAT CAAAGACTATGAAGACCTGCAGTCTGCCATCAGCTTCCTCATAGAAACTGGGACCCTGCTGCACTTTCCA GACACGAGCCATGGCCTAAGGAACCTCTACTTCCTGGATCCCATCTGGCTCTCTGAATGCCTGCAGAGGA TCTTTAACATCAAGGGCTCGCGGTCAGTGGCAAAGAATGGGGTGATCCAAGCGGAAGACCTCAGGATGCT GCTGGTGGGGACAGGCTTCACACAGCAGACTGAGGAGCAATACTTCCAGTTCCTTGCCAAGTTCGAGATT GCCCTCCCTGTTGCCAATGACAGCTACCTCCTACCACACCTCCTTCCATCCAAGCCTGGGCTGGACACCC ACAGCATGCGGCACCCAATGGCTAACACCATCCAGCGGGTGTTTAAGATGAGCTTCGTGCCTGTTGGCTT CTGGCAACGGTTCATAGCACGGATGCTAATCAGCTTGGCTGAGATGGACCTGCAGCTTTTTGAAAACAAG AAGAATACGAAAAGCAGGAACCGGAAAGTCACTATTTACAGTTTTACAGGGAGCCAGAGAAACCGCTGCA GTACATTCCGAGTCCGAAGAAATCAGACCATCTACTGGCAGGAAGGGCTGCTGGTCACTTTCGATGGGGG CTACCTCAGTGTGGAATCCTCAGATGTGAACTGGAAAAAGAAAAAAAGCGGAGGAATTAAAATCATCTGC CAGTCAGAGATGAGGGACTTCTCGGCAATGGCTTTTATCACAGACCATGTCAACTCCCTGATTGACCAGT GGTTCCCTGCCCTGACGGCCACAGAAAGTGACGGGACCCCACTGATGGAGCAGTACGTACCCTGCCCTGT CTGTGAAGCATCCTGGGCCCAGCATGCAGATCCAAACGAGAGGTCAGAGAGCGTGCAGTACTTTGATATG GAAGACTGTGTGCTCACAGCCATCGAACGGGACTTCATCTCCTGCCCCAGACACCCAGACCTCCCAGTAC CACTTCAGGAGCTTGTACCAGAGCTGTTCATGACTGACTTCCCAGCCAGGCTCTTCCTGGAGAACAGCAA GCTGGAGCACACGGAGGGTGAGAACAGCATTCTGGGCCAAGGTGGCAGTGGCACAGTCATCTACCAGGCC CGGTACCAGGGCCAGCCTGTGGCTGTGAAGAGGTTCCATATCAAGAAGTTCAAGAATTCTGCTAATGCCC CAGCAGACACCATGCTGCGGCACCTGAGAGCCATGGATGCCATGAAGAACTTCTCAGATTTCCGTCAGGA GGCCAGCATGCTGCATGCCTTGCAGCATCCTTGCATCGTGTCACTCATCGGCATCAGCATCCACCCGCTC TGCTTCGCCTTGGAGCTGGCCCCGCTGGGCAGTCTCAACACCGTGCTATCTGAGAATGCCAAAGATTCTT CCTTTATGCCCCTGGGACACATGCTCACCCAAAAGATAGCCTACCAGATTGCCTCGGGCCTGGCCTACCT GCACAAGAAAAACATCATCTTCTGTGACCTCAAGTCGGACAACATCCTAGTGTGGTCGCTGAGCGCCAAG GAGCATATCAACATCAAACTGTCAGACTACGGCATCTCCAGGCAGTCCTTTCACGAGGGGGCCCTGGGTG TGGAGGGCACCCCAGGTTACCAGGCCCCAGAGATCAGGCCTCGCATCGTGTATGACGAGAAGGTAGATAT GTTCTCCTACGGCATGGTGCTATACGAGCTGCTGTCAGGACAGCGTCCTGCACTGGGCCACCACCAACTT CAGATTGTCAAGAAGTTGTCCAAGGGCATCCGCCCAGTTCTGGGGCAGCCGGAGGAAGTTCAGTTCCATC GGCTGCAGGCGCTCATGATGGAGTGCTGGGACACAAAGCCGGAGAAGCGCCCCCTGGCCTTGTCCGTGGT GAGCCAGATGAAAGACCCAACCTTTGCCACCTTCATGTACATGCTGCCCTGTGGGAAGCAGTCGGCCTTC TTCTCATCCCAGAGCCAGGAGTACACTGTGGTGTTCTGGGATGGGAAGGAGGAGTCCAGGAATTACACGG TGGTCAACACAGAGAAGGGCCTTCTGGAAGTACAGAGGATGACCTGCCCCGGGATGAAGTTGAGCTGTCA GCTCAAGGTCCAGAGCTCAGTGTGGATAGCCACGGAGGACCAGAAAATCTATATCTACAGCCTTAAGGGT ATGTGCCCATTAAGCGTGCCCCAGCAGGCCCTGGACACCCCAGCTGTCGTCACCTGTTTCTTGGCAGTAC CTGTTATCAAAAAGAACTCCTTCCTGGTGCTGGCAGGCCTGGCTGACGGACTCGTGGCTGTGTTTCCTGT GGCACGGGGCACCCCAAAAGAAAGCTGCTCCTACCTGTGCTCCCACACAGCCAACAGGTCCAAGTTCTGC ATCCCCGACGAAGATGCACGGCAGAACCCTTACCCAGTGAAGGCAATGGAAGTGGTCAACAGTGGGTCTG AGGTCTGGTACAGCAACGGGCCTGGCCTCCTCGTCATTGACTGCACCATCCTGGACATCAGCAGGCGGCT GGAGCCCTACGCAGCTCCGTCCATGGTCACGTCACTTGTGTGCAGCTCAGACTGCAGAGGAGAGGAGATG GTCTGGTGCCTCGACGACAAGGCCAATTGCTTAGTGATGTACCACTCAGCCACCTACCAGCTCTGTGCCC GGTACTTCTGTGGGGACCCCAATCCTCTCAGGGACACGTTTTCTGTGCAGCCTTCAGTCCTAGAAACCCC AGGCAGTCACAAAACCACCTCAAAGGGGCCTGTGGAAGAGTGCATCGCGGATGTGAGCATCATGTACAGC GAGGAGCTGGGCACGCAGATCCTCACCCACCAGGACTCGCTCACCGACTACTGCTCCATGTCCTCTTATT CTTCGTCCCCACCCCACCAAGATCCTAGGTCCCCTTCCAGTCTGCCCAGCTCCCTCACCAGCTATTCCAG TGTGCCTTTCTCTGCCAACTATGAAGACTCAGACAGGCTGCAGGAGCCCAGTGTCACCTCTGACAGAACT GAGCATGACCTGAGCCCCATGGATGGGGAGACGTTCAGCCAGCACCTGCAGGCTGTGAAGGTGCTTGCTG TCAAAGACCTCATTTGGGTTCCTAGACATGGAGGAGATATCATTGTCATCGGCCTGGAGAAGGATTCAGG TGCCCAGCGGGGCAGAGTCATCGCTGTTTTAAAAGCCCGAGAGCTCAATCGGCACGGGGTTCTGGTGGAT GCCGCAGTGGTGGCAAAGGACACCGTAGTGTGCAGCTTTGCAAATGAAAACACAGAGTGGTGCCTGGCTG TCTGGAGGGGCTGGGGCGCCAGGGAGTTTGACATCTTCTACCAGTCCTATGAGGAGCTGGGCCGGCTGGA GGCTTGTACTCGCAAGAGAAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225310 representing NM_146191
Red=Cloning site Green=Tags(s) MAGTSQRPPSMYWCVGTEGLAVCPGPAMETHNGAEDMGSKLSLPGGSSTVQCPSMEEIHTAYKQRNLSRA RDLLRGVCEESESSQEKGQLLSIAAAHGDLETVQFLLTEKRVELPTEPTDDNPAVVAAHFGHAEVVRELL ESLPGPCTPQRLLNWMLALACQRGHLEVVKLLVLTHGADPENYAVRKNEFPVIVRLPLYAAIKAGNEDIA IFLLRHGAYFCSYILLDSPEPSKHLLRKYFIEASALPSSYPGKIALRVKWSHLKLPWVDLDWLLDISCQI TELDLSANCLPSLPSIIPWGLINLKKLNLSNNQLGELPCVQSSDEIICSRLLEIDISSNKLSHLPPGFLH LSKLQKLTASKNYLERLFEEENATNWIGLRKLQELDLADNRLTELPVQFMHSFKSLTSLNVSRNNLKSFP DPWSCPLKCCKASKNALESLPDKMAVFWKSHLRDADFSENSLKEVPLGLFQLDALMFLRLQGNQLLSLPP QEKWTCTQLKTLDLSRNQLGKNEDGLKTKRISLFTTRGRQRSGTETASMLEFPAFLSESLEVLCLNDNHL DAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNISNVPAEVRKEGPKATLSFLRAQL RKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQLAHSEATIRTTKWELQRPAGSKAKVESVEFNVWDI GGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVER IATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAG RLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFP DTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEI ALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENK KNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIIC QSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDM EDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQA RYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPL CFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSAK EHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQL QIVKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAF FSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKG MCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSYLCSHTANRSKFC IPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRRLEPYAAPSMVTSLVCSSDCRGEEM VWCLDDKANCLVMYHSATYQLCARYFCGDPNPLRDTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYS EELGTQILTHQDSLTDYCSMSSYSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRT EHDLSPMDGETFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVLVD AAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_146191 |
| ORF Size | 6042 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_146191.3, NP_666303.3 |
| RefSeq Size | 7467 bp |
| RefSeq ORF | 6045 bp |
| Locus ID | 233328 |
| UniProt ID | Q3UHC2 |
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