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Lrp4 (NM_001145857) Mouse Tagged ORF Clone
CAT＃: MG222184
- TrueORF®
Lrp4 (tGFP-tagged) - Mouse low density lipoprotein receptor-related protein 4 (Lrp4) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001145857" in other vectors (2)
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CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6430526J12Rik; D230026E03; mdig; Megf7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222184 representing NM_001145857
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGTGGTGGGGCGCGCTGCTGCTTGGCGCCCTGCTCTGCGCACACGGAATAGCCAGCAGTCTGG AGTGTGCATGTGGTCGGAGCCACTTCACATGTGCGGTGAGCGCGCTTGGCGAATGTACCTGTATCCCCGC CCAGTGGCAGTGTGATGGAGACAATGACTGTGGAGACCACAGCGATGAGGATGGCTGTACGCTGCCTACC TGCTCCCCTCTGGACTTCCACTGTGACAACGGCAAGTGCATCCGACGCTCCTGGGTGTGTGACGGGGACA ATGACTGTGAAGATGACTCTGATGAGCAGGACTGTCCCCCCCGGGAGTGTGAGGAGGACGAATTCCCCTG CCAGAACGGCTACTGCATTCGGAGTCTGTGGCACTGTGATGGGGACAACGACTGTGGTGACAACAGTGAC GAGCAATGCGACATGCGCAAGTGCTCAGACAAGGAATTCCGCTGCAGCGACGGCAGCTGCATTGCTGAGC ATTGGTACTGCGATGGTGACACAGACTGCAAAGATGGCTCTGATGAGGAAAGCTGCCCTTCAGCAGTACC CTCTCCTCCTTGCAACCTGGAAGAGTTCCAGTGCGCCTATGGCCGCTGCATCCTCGACATCTACCACTGT GACGGAGATGACGACTGTGGAGACTGGTCAGACGAGTCTGACTGCTCCTCCCACCAGCCCTGCCGCTCTG GGGAATTCATGTGTGACAGTGGCCTGTGCATCAACTCGGGCTGGCGCTGTGATGGTGATGCAGACTGTGA TGACCAGTCTGATGAACGCAACTGCACCACCTCCATGTGCACAGCCGAACAGTTTCGCTGTCGGTCAGGC CGTTGCGTCCGTCTGTCCTGGCGCTGTGATGGGGAGGATGACTGTGCGGACAACAGCGATGAAGAAAACT GTGAGAACACAGGAAGCCCCCAGTGCGCCTCTGACCAGTTCCTGTGTTGGAACGGGCGCTGTATTGGGCA GAGGAAGCTGTGCAACGGGATCAACGACTGCGGTGACAGCAGTGACGAAAGTCCACAGCAGAACTGTCGG CCCCGGACGGGTGAGGAGAACTGCAATGTTAACAACGGTGGCTGTGCCCAGAAGTGTCAGATGGTGCGAG GGGCAGTGCAGTGTACCTGCCACACAGGCTACCGGCTCACAGAGGATGGGAGAACGTGCCAGGATGTAAA CGAATGTGCTGAGGAGGGGTACTGCAGCCAGGGCTGCACAAACACTGAAGGGGCTTTCCAGTGCTGGTGT GAAGCAGGGTATGAGCTACGGCCCGATCGGCGCAGCTGCAAAGCTCTGGGGCCAGAACCTGTGCTACTGT TTGCCAATCGTATCGACATCCGGCAGGTGCTCCCGCACCGCTCCGAGTACACGCTGCTACTGAACAACCT GGAGAACGCCATTGCCCTCGACTTTCATCACCGGCGGGAGCTGGTCTTCTGGTCGGATGTCACCCTGGAC CGCATCCTCCGTGCCAACCTTAATGGCAGCAATGTAGAGGAGGTGGTGTCTACTGGGCTAGAGAGCCCAG GTAGGAAAACAAGGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222184 representing NM_001145857
Red=Cloning site Green=Tags(s) MRRWWGALLLGALLCAHGIASSLECACGRSHFTCAVSALGECTCIPAQWQCDGDNDCGDHSDEDGCTLPT CSPLDFHCDNGKCIRRSWVCDGDNDCEDDSDEQDCPPRECEEDEFPCQNGYCIRSLWHCDGDNDCGDNSD EQCDMRKCSDKEFRCSDGSCIAEHWYCDGDTDCKDGSDEESCPSAVPSPPCNLEEFQCAYGRCILDIYHC DGDDDCGDWSDESDCSSHQPCRSGEFMCDSGLCINSGWRCDGDADCDDQSDERNCTTSMCTAEQFRCRSG RCVRLSWRCDGEDDCADNSDEENCENTGSPQCASDQFLCWNGRCIGQRKLCNGINDCGDSSDESPQQNCR PRTGEENCNVNNGGCAQKCQMVRGAVQCTCHTGYRLTEDGRTCQDVNECAEEGYCSQGCTNTEGAFQCWC EAGYELRPDRRSCKALGPEPVLLFANRIDIRQVLPHRSEYTLLLNNLENAIALDFHHRRELVFWSDVTLD RILRANLNGSNVEEVVSTGLESPGRKTRP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001145857 |
| ORF Size | 1557 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001145857.1, NP_001139329.1 |
| RefSeq Size | 3246 bp |
| RefSeq ORF | 1560 bp |
| Locus ID | 228357 |
| UniProt ID | Q8VI56 |
| Gene Summary | Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. Plays an essential role in the process of digit differentiation (PubMed:16517118).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217359 | Lrp4 (untagged) - Mouse low density lipoprotein receptor-related protein 4 (Lrp4), transcript variant 2, (10ug) |
CNY 3,872.00 |
|
| MR222184 | Lrp4 (Myc-DDK-tagged) - Mouse low density lipoprotein receptor-related protein 4 (Lrp4), transcript variant 2 |
CNY 5,130.00 |
