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Wnk1 (NM_001185021) Mouse Tagged ORF Clone
CAT＃: MG221514
- TrueORF®
Wnk1 (tGFP-tagged) - Mouse WNK lysine deficient protein kinase 1 (Wnk1) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001185021" in other vectors (2)
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CNY 24,040.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6430573H23Rik; EG406236; Hsn2; mKIAA0344; Prkwnk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221514 representing NM_001185021
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGTGCCGCCGAGAAGCAAAGCGGCACTCCGGGCTTCCTTACGCCTCCGGCGCCCGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCTACGGTCGCCGACTCGGGAGTTGG CAGGACGGAGGAATATCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACC ACCCCCACGGAGCACCGATTCTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACCGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCGCAACCTAGTGTACCTGCAGTGGTGCCCCAGAGTGCTCCACCCGAACC TCACAGAGAGGAAACTTTGACCGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCACGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGGAGCAAAGAGGAGCCACCGCCGTCCAGAAGTGGAAGTGGCAGTGGTGG CGCCAGCGCCAAAGAGGCGCAGGAGGATAGGAGCCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CAGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAGGCTGAAATGTTAAAGGGTCTTCAACATCCCAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAGACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGGCTTCAGTTTCTCCACACACGAACTCCACCAATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACTGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAATATGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATAT CCATACTCAGAATGCCAAAATGCTGCGCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTAAAAGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATTAAATTATGGCTGCGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTTGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTGTCCTTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAGCAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGCGTTTCCCAGGCAGGAATCCAGCAGCT CTCTGCTGCTAGCACTGGCATACCCACTGCTCCTGCCACCTCTGCTTCAGTTTCTACACAGGTAGAACCT GAAGAACCTGAGGCAGATCAACATCAACAGCTGCAATATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACCATTGATAGTGGTCAAGGATCATCCGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGTTCCCAACATGAGCAGGCACATTCTACTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGGCACAGGGGCAGAACCAGGGCCAGCCAT CAAGTAGTTTGGCTGGGGTTCTATCTTCACAACCTATCCAACATCCTCAGCAGCAGGGAATACAGCCAAC TGTTCCTTCTCAACAGGCAGTACAGTATTCACTTCCACAAGCAGCATCTTCCAGTGAAGGTACTACTGCA CAGCCAGTGAGTCAGCCTCAAGTGTCAGCTGGAACACAGGGCTTCCCATCTCGACTGCCACCACAATACC CAGGAGATTCAAATATTGCTCCCTCTTCCAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTAGCCCC TCCTTCTATGCCGACAGAAGCATTGGCCACCCAAGGTTACTTTCCTACAGTAGTGCAGCCTTATGTGGAA TCAACTCCTTTGGTTCCTATGGGCAGTGTAGGAGGCCAGGTTCAAGTGTCCCAACCAGCAGTGAGTTTAA CGCAACAACCCCCAACTACATCCTCCCAGCAAGCAGTTCTGGAGAGTACTCAGGGAGTCTCTCAGGCTGC TCCTCCAGAACAAACTCCAATAACACAGTCACAACCTACTCAGCCTGTTCCATTGGTTACCTCTGCAGAC AGTGCACATTCAGACGTTGCTTCTGGTATGAGTGATGGCAATGAGAATGCTCCATCATCCAGCGGGAGGC ATGAAGGGAGGACAACAAAACGGCATTATAGAAAATCCGTAAGAAGTCGCTCTCGGCATGAAAAGACTTC TCGCCCAAAACTGAGGATTTTAAATGTTTCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACT CATAATCGGAAGATGGTTACATTTAAATTTGACCTAGATGGTGACAACCCTGAAGAGATAGCAACAATTA TGGTGAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCTTTTGTGGCTCAAGTGCGGGAAATTATTGA AAAGGCTGATGAAATGTTAAGTGAGGATGTCAGTGTGGAGCCTGAGGGTGACCAGGGATTGGAGAGTCTG CAAGGAAAGGATGACTATGGCTTCCCAGGTTCTCAGAAATTAGAAGGAGAGTTCAAGCAACCAATTGCTG TATCTTCCATGCCACAACAAATAGGTGTTCCTACCAGTTCTTTAACGCAAGTTGTTCATTCTGCTGGAAG ACGGTTTATAGTGAGTCCTGTACCTGAAAGTAGGTTACGAGAATCAAAAGTTTTCACCAGTGACATATCA GATCCAGTTGTGGCCTCTACATCTCAGGCCCCTGGAATGAACTTATCTCACTCTGCTTCATCCCTCAGTC TCCAGCAGGCTTTTTCTGAACTGAAACATGGTCAGATGACTGAAGGACCCAATACAGCACCTCCAAATTT TAATCACATGGCGGGACCAACATTTTCTCCCTTTTTGGCTAGCATTGCTGGTGTCCAAACTGTAGCAGCA TCCACACCGTCAGTATCAGTCCCTATAACAAGCAGTCCTCTTAATGACATTTCCACATCAGTGATGCAGT CTGAGACTGCACTGCCCACTGAAAAAGGCATCGTGGGAGTTACCACTACCAGCACAGGTGTGGTAGCTTC AGGTGGTCTCACCACAATGTCTGTGTCTGAGTCACCAACATCTTCCAGTGCGGTTTCAAGTTCTACAGTA CCTGCAGTTGTCACTGTGTCCACACCATCCCAGCCAGTTCAGGCTTCCACATCTGGGAGCATTGCTTCAA GTACAGGCAGTTTTCCTCCTGGAACATTTTCTACAACTACAGCTACTACAATGGGTAGTGTGGTGGCCCC AGATGCTAAGCCTCCAACTGTATTATTGCAACAGGTAGCAAGCAATACTGCTGGGGTTGCCATAGTGACA TCAGTCTCTACCACCACTCCATTCCCAGGCATGGCTTCACAGCCATCCCTTCCACTTAGTAGCAGCACCT CTGCTCCTACTCTAGCTGAAACAATGGTGGTTAGTGCACACTCACTAGACAAGGCATCTCACAGTAGTAC AGCAGGATTGGGTTTGTCCTTTTGTGCGCCATCCTCTTCCTCCTCCTCTGGAACAGCTGTGTCTACTTCT GTTTCTCAGCCTGGTATGGTTCATCCTTTGGTCATCTCATCAGCTGTAGTTTCTACACCTGGCCTCCCCC AACCAGTAGTACCTACTTCTACACCTTTATTACCCCAAGTACCTAATATCCCACCCTTGGTACAACCTGT TGTTAATGTGCCTGCTGTACAGCAAACACTCATTCACAGTCAGCCTCAACCAGCTCTGCTTCCCAACCAG CCTCACACACACTGTCCTGAAATGGATGCTGATACCCAATCCAAAGCTCCTGGGATTGATGATATAAAGA CTCTGGAGGAAAAGCTGAGATCTCTCTTTAGTGAACACAGCTCATCTGGAACTCAGCATGCTTCTGTCTC CCTAGAGACACCTTTAGTAGTAGAGACGACTGTCACACCGGGCATCACTACCACTGCTGTAGCACCAAGT AAACTCATGACTTCCACTACAAGTACTTGCTTACCACCAACCAGTTTACCATTAGGAGCAGCTGGCATGC CAGTTATGCCAGTAGGCACACCTGGGCAGGTTTCTACCCCAGGCACACACGCTTCAGCCCCAGTCGGCAC TGCAACAGGAGTAAAACCTGGAACAACTCCCCCAAAGCCAACTAAGACTGTGGTGCCACCAGTGGGTACT GAACTTTCAGCCGGTACTGTACCCTGTGAGCAGCTGCCACCTTTTCCTGGACCTTCTCTAATTCAGCCTT TGTCTACGATGTCTTCAACAACAGTTACAGAAGCAGGGACTCGGCTTCAAAAGGATGGCACAGAAGGTCA TGTCACAGCAACTAGTTCAGGAGCTGGTGTTGTTAAGATGGGGCGATTTCAGGTTTCAGTTACAATGGAT GATGCCCAGAAAGAACGTAAAAATAGGTCAGAAGATACAAAGTCGGTTCATTTTGAATCTAGCACATCAG AGTCATCAGTTCTTTCAAGTAGTAGTCCAGAGAGTACCCTGGTAAAGCCAGAGCCTAATGGAATTAGTAT TTCTGGCATCTCCTTAGATGTCCCAGACAGCACCCACAAAGCACCTACCCCAGAAGCAAAGTCAGATGCA GGACAGCCTACCAAGGTGGGACGTTTTCAGGTGACAACTACAGCCAACAAAGTGGGTCGTTTCTCTGTTT CAAGAACTGAGGACAAGGTCACAGAGCTCAAAAAAGAGGGACCAGTGACCTCTCCTCCTTTCAGAGATTC AGAACAAACTGTTATTCCTGCTGTGATACCAAAGAAGGAGAAGCCTGAGCTGGCAGAACCTTCCCATTTG AATGGGCCATCTTCAGACCTGGAGGCTGCCTTTCTAAGTAGGGGTACAGAGGATGGCTCAGGTAGTCCAC ACTCTCCCCCTCACCTGTGTTCAAAGAGCCTTCCCGTCCAGAATCTAAGTCAAAGCCTTAGTAATTCATT CAACTCCTCATACATGAGTAGTGACAATGAGTCCGACATTGAAGATGAAGACTTAAGGTTAGAACTACGA CGACTACGAGAAAAACATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTT TGTATACTAAACTGGGCAAGGTTCCCCCTGCTGTCATTATTCCCCCAGCTGCTCCTCTGTCGGGGAGAAG AAGGAGACCCACTAAAAGCAAAGGCAGCAAGTCTAGTCGCAGCAGCTCATTGGGCAATAAAAGCCCACAG CTTTCAGGCAACCTGTCTGGTCAGAGTGGAACTTCAGTCTTACACCCCCAACAGACCCTCCATCCCGCAG GAAACACCCCCGAGACTGGGCACAACCAGCTGTTACAGCCTCTTAAGCCATCTCCCTCCAGTGACAACCT CTATTCAGCCTTTACCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGGACCAGC AGCACAAATACCGTTGGAGGAACAGTGAGTAGCCAGGCTGCCCAGGCTCAGCCTCCTGCTATGACTTCCA GTAGGAAGGGCACATTCACAGATGACCTGCACAAGTTAGTAGACAACTGGGCCCGAGATGCCATGAATCT TTCAGGCAGGAGAGGCAGCAAAGGACACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCT CCTGGGCAGCTGTGTGTTCCCATGACCTCAAACCTGGGTGGCTCTACCCCCATCTCTGCAGCATCTGCTA CCTCTCTAGGTCACTTCACCAAATCCATGTGTCCCCCACAGCAGTATGGTTTTCCACCTGCCCCATTTGG CACTCAGTGGAGTGGAACAGGTGGTCCAGCACCGCAGCCACTTGGCCAGTTCCAACCTGTAGGAACAGCA TCCTTGCAAAATTTCAACATCAGCAATTTGCAGAAGTCCATCAGCAACCCCCCAGGTTCCAATCTACGGA CCACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221514 representing NM_001185021
Red=Cloning site Green=Tags(s) MSDGAAEKQSGTPGFLTPPAPVPKNGSSSDSSVGEKLGATVADSGVGRTEEYRRRRHTMDKDSRGAAATT TPTEHRFFRRSVICDSNATALELPGLPLSIPQPSVPAVVPQSAPPEPHREETLTATVASQVSQQPSAAAS PGEQAVVGSATTTVPSSTSKDRPVSQPSLVGSKEEPPPSRSGSGSGGASAKEAQEDRSQQQDDIEELETK AVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNI VRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDL KCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEY PYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELA EEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKD RVSLIKRKREQRQLVREEQEKRKQEESSFKQQNEQQASVSQAGIQQLSAASTGIPTAPATSASVSTQVEP EEPEADQHQQLQYQQPSISVLSDGTIDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTAPGHTVSSIQ AQSQPHGVYPPSSMAQGQNQGQPSSSLAGVLSSQPIQHPQQQGIQPTVPSQQAVQYSLPQAASSSEGTTA QPVSQPQVSAGTQGFPSRLPPQYPGDSNIAPSSNVASVCIHSTVLAPPSMPTEALATQGYFPTVVQPYVE STPLVPMGSVGGQVQVSQPAVSLTQQPPTTSSQQAVLESTQGVSQAAPPEQTPITQSQPTQPVPLVTSAD SAHSDVASGMSDGNENAPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLET HNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVAQVREIIEKADEMLSEDVSVEPEGDQGLESL QGKDDYGFPGSQKLEGEFKQPIAVSSMPQQIGVPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSDIS DPVVASTSQAPGMNLSHSASSLSLQQAFSELKHGQMTEGPNTAPPNFNHMAGPTFSPFLASIAGVQTVAA STPSVSVPITSSPLNDISTSVMQSETALPTEKGIVGVTTTSTGVVASGGLTTMSVSESPTSSSAVSSSTV PAVVTVSTPSQPVQASTSGSIASSTGSFPPGTFSTTTATTMGSVVAPDAKPPTVLLQQVASNTAGVAIVT SVSTTTPFPGMASQPSLPLSSSTSAPTLAETMVVSAHSLDKASHSSTAGLGLSFCAPSSSSSSGTAVSTS VSQPGMVHPLVISSAVVSTPGLPQPVVPTSTPLLPQVPNIPPLVQPVVNVPAVQQTLIHSQPQPALLPNQ PHTHCPEMDADTQSKAPGIDDIKTLEEKLRSLFSEHSSSGTQHASVSLETPLVVETTVTPGITTTAVAPS KLMTSTTSTCLPPTSLPLGAAGMPVMPVGTPGQVSTPGTHASAPVGTATGVKPGTTPPKPTKTVVPPVGT ELSAGTVPCEQLPPFPGPSLIQPLSTMSSTTVTEAGTRLQKDGTEGHVTATSSGAGVVKMGRFQVSVTMD DAQKERKNRSEDTKSVHFESSTSESSVLSSSSPESTLVKPEPNGISISGISLDVPDSTHKAPTPEAKSDA GQPTKVGRFQVTTTANKVGRFSVSRTEDKVTELKKEGPVTSPPFRDSEQTVIPAVIPKKEKPELAEPSHL NGPSSDLEAAFLSRGTEDGSGSPHSPPHLCSKSLPVQNLSQSLSNSFNSSYMSSDNESDIEDEDLRLELR RLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQ LSGNLSGQSGTSVLHPQQTLHPAGNTPETGHNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTS STNTVGGTVSSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSA PGQLCVPMTSNLGGSTPISAASATSLGHFTKSMCPPQQYGFPPAPFGTQWSGTGGPAPQPLGQFQPVGTA SLQNFNISNLQKSISNPPGSNLRTT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001185021 |
| ORF Size | 6585 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001185021.1, NP_001171950.1 |
| RefSeq Size | 10029 bp |
| RefSeq ORF | 6588 bp |
| Locus ID | 232341 |
| UniProt ID | P83741 |
| Gene Summary | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537, PubMed:23542070).[UniProtKB/Swiss-Prot Function] |
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