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Wnk1 (NM_198703) Mouse Tagged ORF Clone
CAT＃: MG221511
- TrueORF®
Wnk1 (tGFP-tagged) - Mouse WNK lysine deficient protein kinase 1 (Wnk1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_198703" in other vectors (2)
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CNY 26,130.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6430573H23Rik; EG406236; Hsn2; mKIAA0344; Prkwnk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221511 representing NM_198703
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGTGCCGCCGAGAAGCAAAGCGGCACTCCGGGCTTCCTTACGCCTCCGGCGCCCGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCTACGGTCGCCGACTCGGGAGTTGG CAGGACGGAGGAATATCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACC ACCCCCACGGAGCACCGATTCTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACCGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCGCAACCTAGTGTACCTGCAGTGGTGCCCCAGAGTGCTCCACCCGAACC TCACAGAGAGGAAACTTTGACCGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCACGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGGAGCAAAGAGGAGCCACCGCCGTCCAGAAGTGGAAGTGGCAGTGGTGG CGCCAGCGCCAAAGAGGCGCAGGAGGATAGGAGCCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CAGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAGGCTGAAATGTTAAAGGGTCTTCAACATCCCAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAGACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGGCTTCAGTTTCTCCACACACGAACTCCACCAATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACTGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAATATGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATAT CCATACTCAGAATGCCAAAATGCTGCGCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTAAAAGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATTAAATTATGGCTGCGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTTGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTGTCCTTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAGCAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGCGTTTCCCAGGCAGGAATCCAGCAGCT CTCTGCTGCTAGCACTGGCATACCCACTGCTCCTGCCACCTCTGCTTCAGTTTCTACACAGGTAGAACCT GAAGAACCTGAGGCAGATCAACATCAACAGCTGCAATATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACCATTGATAGTGGTCAAGGATCATCCGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGTTCCCAACATGAGCAGGCACATTCTACTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGGCACAGGGGCAGAACCAGGGCCAGCCAT CAAGTAGTTTGGCTGGGGTTCTATCTTCACAACCTATCCAACATCCTCAGCAGCAGGGAATACAGCCAAC TGTTCCTTCTCAACAGGCAGTACAGTATTCACTTCCACAAGCAGCATCTTCCAGTGAAGGTACTACTGCA CAGCCAGTGAGTCAGCCTCAAGTGTCAGCTGGAACACAGCTTCCAGTTTCCCAGACAGTAGCAACTGTCC AAGGTGAACCTCACATCCCAGTTTCAACACAACCCTCAGTTGTTCCAGTCCACTCTGGTGCTCATTTCCT TCCTATGGGACAGCCAATCCCTACTTCTTTACTCCCACAGTATCCTGTCTCTCAAATTCCCATATCGACT CCTCATGTGTCCACGGCGCAGACAGGTTTCTCATCCGTTCCTATAACAATGGCTGCTGGCATTAACCAGC CTCTGCTCACCTTGGCTTCATCTGCTACAGCATCTTCAATCCCAGGGGGATCACCTGTAGTTCCTAATCA GCTTCCAACTCTTCTGCAGCCTGTGAATCAGCTGCAGAGTCAGGTTCACCCACAGCTTCTACAGCCAACA ACAGTTCAGTCCATAGGAATACCAGCTAACCTTGGACAAGCTGCTGAGGGTCCACTTCCCTCTGGAGATG TTTTGTACCAGGGCTTCCCATCTCGACTGCCACCACAATACCCAGGAGATTCAAATATTGCTCCCTCTTC CAACGTGGCTTCTGTTTGCATCCATTCTACAGTCCTAGCCCCTCCTTCTATGCCGACAGAAGCATTGGCC ACCCAAGGTTACTTTCCTACAGTAGTGCAGCCTTATGTGGAATCAACTCCTTTGGTTCCTATGGGCAGTG TAGGAGGCCAGGTTCAAGTGTCCCAACCAGCAGTGAGTTTAACGCAACAACCCCCAACTACATCCTCCCA GCAAGCAGTTCTGGAGAGTACTCAGGGAGTCTCTCAGGCTGCTCCTCCAGAACAAACTCCAATAACACAG TCACAACCTACTCAGCCTGTTCCATTGGTTACCTCTGCAGACAGTGCACATTCAGACGTTGCTTCTGGTA TGAGTGATGGCAATGAGAATGCTCCATCATCCAGCGGGAGGCATGAAGGGAGGACAACAAAACGGCATTA TAGAAAATCCGTAAGAAGTCGCTCTCGGCATGAAAAGACTTCTCGCCCAAAACTGAGGATTTTAAATGTT TCAAATAAAGGAGACCGAGTAGTAGAATGTCAATTAGAGACTCATAATCGGAAGATGGTTACATTTAAAT TTGACCTAGATGGTGACAACCCTGAAGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAAT AGAGAGAGAGTCTTTTGTGGCTCAAGTGCGGGAAATTATTGAAAAGGCTGATGAAATGTTAAGTGAGGAT GTCAGTGTGGAGCCTGAGGGTGACCAGGGATTGGAGAGTCTGCAAGGAAAGGATGACTATGGCTTCCCAG GTTCTCAGAAATTAGAAGGAGAGTTCAAGCAACCAATTGCTGTATCTTCCATGCCACAACAAATAGGTGT TCCTACCAGTTCTTTAACGCAAGTTGTTCATTCTGCTGGAAGACGGTTTATAGTGAGTCCTGTACCTGAA AGTAGGTTACGAGAATCAAAAGTTTTCACCAGTGACATATCAGATCCAGTTGTGGCCTCTACATCTCAGG CCCCTGGAATGAACTTATCTCACTCTGCTTCATCCCTCAGTCTCCAGCAGGCTTTTTCTGAACTGAAACA TGGTCAGATGACTGAAGGACCCAATACAGCACCTCCAAATTTTAATCACATGGCGGGACCAACATTTTCT CCCTTTTTGGCTAGCATTGCTGGTGTCCAAACTGTAGCAGCATCCACACCGTCAGTATCAGTCCCTATAA CAAGCAGTCCTCTTAATGACATTTCCACATCAGTGATGCAGTCTGAGACTGCACTGCCCACTGAAAAAGG CATCGTGGGAGTTACCACTACCAGCACAGGTGTGGTAGCTTCAGGTGGTCTCACCACAATGTCTGTGTCT GAGTCACCAACATCTTCCAGTGCGGTTTCAAGTTCTACAGTACCTGCAGTTGTCACTGTGTCCACACCAT CCCAGCCAGTTCAGGCTTCCACATCTGGGAGCATTGCTTCAAGTACAGGCAGTTTTCCTCCTGGAACATT TTCTACAACTACAGCTACTACAATGGGTAGTGTGGTGGCCCCAGATGCTAAGCCTCCAACTGTATTATTG CAACAGGTAGCAAGCAATACTGCTGGGGTTGCCATAGTGACATCAGTCTCTACCACCACTCCATTCCCAG GCATGGCTTCACAGCCATCCCTTCCACTTAGTAGCAGCACCTCTGCTCCTACTCTAGCTGAAACAATGGT GGTTAGTGCACACTCACTAGACAAGGCATCTCACAGTAGTACAGCAGGATTGGGTTTGTCCTTTTGTGCG CCATCCTCTTCCTCCTCCTCTGGAACAGCTGTGTCTACTTCTGTTTCTCAGCCTGGTATGGTTCATCCTT TGGTCATCTCATCAGCTGTAGTTTCTACACCTGGCCTCCCCCAACCAGTAGTACCTACTTCTACACCTTT ATTACCCCAAGTACCTAATATCCCACCCTTGGTACAACCTGTTGTTAATGTGCCTGCTGTACAGCAAACA CTCATTCACAGTCAGCCTCAACCAGCTCTGCTTCCCAACCAGCCTCACACACACTGTCCTGAAATGGATG CTGATACCCAATCCAAAGCTCCTGGGATTGATGATATAAAGACTCTGGAGGAAAAGCTGAGATCTCTCTT TAGTGAACACAGCTCATCTGGAACTCAGCATGCTTCTGTCTCCCTAGAGACACCTTTAGTAGTAGAGACG ACTGTCACACCGGGCATCACTACCACTGCTGTAGCACCAAGTAAACTCATGACTTCCACTACAAGTACTT GCTTACCACCAACCAGTTTACCATTAGGAGCAGCTGGCATGCCAGTTATGCCAGTAGGCACACCTGGGCA GGTTTCTACCCCAGGCACACACGCTTCAGCCCCAGTCGGCACTGCAACAGGAGTAAAACCTGGAACAACT CCCCCAAAGCCAACTAAGACTGTGGTGCCACCAGTGGGTACTGAACTTTCAGCCGGTACTGTACCCTGTG AGCAGCTGCCACCTTTTCCTGGACCTTCTCTAATTCAGTCCCAGCAACCACTAGAAGATCTTGATGCTCA GTTGAGAAGAACACTTAGTCCAGAGACTATTACAGTGGCACCTGCAGTTGGTCCTTTGTCTACGATGTCT TCAACAACAGTTACAGAAGCAGGGACTCGGCTTCAAAAGGATGGCACAGAAGGTCATGTCACAGCAACTA GTTCAGGAGCTGGTGTTGTTAAGATGGGGCGATTTCAGGTTTCAGTTACAATGGATGATGCCCAGAAAGA ACGTAAAAATAGGTCAGAAGATACAAAGTCGGTTCATTTTGAATCTAGCACATCAGAGTCATCAGTTCTT TCAAGTAGTAGTCCAGAGAGTACCCTGGTAAAGCCAGAGCCTAATGGAATTAGTATTTCTGGCATCTCCT TAGATGTCCCAGACAGCACCCACAAAGCACCTACCCCAGAAGCAAAGTCAGATGCAGGACAGCCTACCAA GGTGGGACGTTTTCAGGTGACAACTACAGCCAACAAAGTGGGTCGTTTCTCTGTTTCAAGAACTGAGGAC AAGGTCACAGAGCTCAAAAAAGAGGGACCAGTGACCTCTCCTCCTTTCAGAGATTCAGAACAAACTGTTA TTCCTGCTGTGATACCAAAGAAGGAGAAGCCTGAGCTGGCAGAACCTTCCCATTTGAATGGGCCATCTTC AGACCTGGAGGCTGCCTTTCTAAGTAGGGGTACAGAGGATGGCTCAGGTAGTCCACACTCTCCCCCTCAC CTGTGTTCAAAGAGCCTTCCCGTCCAGAATCTAAGTCAAAGCCTTAGTAATTCATTCAACTCCTCATACA TGAGTAGTGACAATGAGTCCGACATTGAAGATGAAGACTTAAGGTTAGAACTACGACGACTACGAGAAAA ACATCTTAAAGAGATTCAGGACCTGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACTAAACTG GGCAAGGTTCCCCCTGCTGTCATTATTCCCCCAGCTGCTCCTCTGTCGGGGAGAAGAAGGAGACCCACTA AAAGCAAAGGCAGCAAGTCTAGTCGCAGCAGCTCATTGGGCAATAAAAGCCCACAGCTTTCAGGCAACCT GTCTGGTCAGAGTGGAACTTCAGTCTTACACCCCCAACAGACCCTCCATCCCGCAGGAAACACCCCCGAG ACTGGGCACAACCAGCTGTTACAGCCTCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTTA CCAGTGATGGTGCCATTTCAGTACCAAGCCTTTCTGCTCCAGGTCAAGGGACCAGCAGCACAAATACCGT TGGAGGAACAGTGAGTAGCCAGGCTGCCCAGGCTCAGCCTCCTGCTATGACTTCCAGTAGGAAGGGCACA TTCACAGATGACCTGCACAAGTTAGTAGACAACTGGGCCCGAGATGCCATGAATCTTTCAGGCAGGAGAG GCAGCAAAGGACACATGAATTATGAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCTCCTGGGCAGCTGTG TGTTCCCATGACCTCAAACCTGGGTGGCTCTACCCCCATCTCTGCAGCATCTGCTACCTCTCTAGGTCAC TTCACCAAATCCATGTGTCCCCCACAGCAGTATGGTTTTCCACCTGCCCCATTTGGCACTCAGTGGAGTG GAACAGGTGGTCCAGCACCGCAGCCACTTGGCCAGTTCCAACCTGTAGGAACAGCATCCTTGCAAAATTT CAACATCAGCAATTTGCAGAAGTCCATCAGCAACCCCCCAGGTTCCAATCTACGGACCACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221511 representing NM_198703
Red=Cloning site Green=Tags(s) MSDGAAEKQSGTPGFLTPPAPVPKNGSSSDSSVGEKLGATVADSGVGRTEEYRRRRHTMDKDSRGAAATT TPTEHRFFRRSVICDSNATALELPGLPLSIPQPSVPAVVPQSAPPEPHREETLTATVASQVSQQPSAAAS PGEQAVVGSATTTVPSSTSKDRPVSQPSLVGSKEEPPPSRSGSGSGGASAKEAQEDRSQQQDDIEELETK AVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNI VRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDL KCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEY PYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELA EEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKD RVSLIKRKREQRQLVREEQEKRKQEESSFKQQNEQQASVSQAGIQQLSAASTGIPTAPATSASVSTQVEP EEPEADQHQQLQYQQPSISVLSDGTIDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTAPGHTVSSIQ AQSQPHGVYPPSSMAQGQNQGQPSSSLAGVLSSQPIQHPQQQGIQPTVPSQQAVQYSLPQAASSSEGTTA QPVSQPQVSAGTQLPVSQTVATVQGEPHIPVSTQPSVVPVHSGAHFLPMGQPIPTSLLPQYPVSQIPIST PHVSTAQTGFSSVPITMAAGINQPLLTLASSATASSIPGGSPVVPNQLPTLLQPVNQLQSQVHPQLLQPT TVQSIGIPANLGQAAEGPLPSGDVLYQGFPSRLPPQYPGDSNIAPSSNVASVCIHSTVLAPPSMPTEALA TQGYFPTVVQPYVESTPLVPMGSVGGQVQVSQPAVSLTQQPPTTSSQQAVLESTQGVSQAAPPEQTPITQ SQPTQPVPLVTSADSAHSDVASGMSDGNENAPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNV SNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVAQVREIIEKADEMLSED VSVEPEGDQGLESLQGKDDYGFPGSQKLEGEFKQPIAVSSMPQQIGVPTSSLTQVVHSAGRRFIVSPVPE SRLRESKVFTSDISDPVVASTSQAPGMNLSHSASSLSLQQAFSELKHGQMTEGPNTAPPNFNHMAGPTFS PFLASIAGVQTVAASTPSVSVPITSSPLNDISTSVMQSETALPTEKGIVGVTTTSTGVVASGGLTTMSVS ESPTSSSAVSSSTVPAVVTVSTPSQPVQASTSGSIASSTGSFPPGTFSTTTATTMGSVVAPDAKPPTVLL QQVASNTAGVAIVTSVSTTTPFPGMASQPSLPLSSSTSAPTLAETMVVSAHSLDKASHSSTAGLGLSFCA PSSSSSSGTAVSTSVSQPGMVHPLVISSAVVSTPGLPQPVVPTSTPLLPQVPNIPPLVQPVVNVPAVQQT LIHSQPQPALLPNQPHTHCPEMDADTQSKAPGIDDIKTLEEKLRSLFSEHSSSGTQHASVSLETPLVVET TVTPGITTTAVAPSKLMTSTTSTCLPPTSLPLGAAGMPVMPVGTPGQVSTPGTHASAPVGTATGVKPGTT PPKPTKTVVPPVGTELSAGTVPCEQLPPFPGPSLIQSQQPLEDLDAQLRRTLSPETITVAPAVGPLSTMS STTVTEAGTRLQKDGTEGHVTATSSGAGVVKMGRFQVSVTMDDAQKERKNRSEDTKSVHFESSTSESSVL SSSSPESTLVKPEPNGISISGISLDVPDSTHKAPTPEAKSDAGQPTKVGRFQVTTTANKVGRFSVSRTED KVTELKKEGPVTSPPFRDSEQTVIPAVIPKKEKPELAEPSHLNGPSSDLEAAFLSRGTEDGSGSPHSPPH LCSKSLPVQNLSQSLSNSFNSSYMSSDNESDIEDEDLRLELRRLREKHLKEIQDLQSRQKHEIESLYTKL GKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSGTSVLHPQQTLHPAGNTPE TGHNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGGTVSSQAAQAQPPAMTSSRKGT FTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCVPMTSNLGGSTPISAASATSLGH FTKSMCPPQQYGFPPAPFGTQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198703 |
| ORF Size | 7131 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198703.3, NP_941992.2 |
| RefSeq Size | 10575 bp |
| RefSeq ORF | 7134 bp |
| Locus ID | 232341 |
| UniProt ID | P83741 |
| Gene Summary | Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537, PubMed:23542070).[UniProtKB/Swiss-Prot Function] |
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