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Mical1 (NM_001164433) Mouse Tagged ORF Clone
CAT＃: MG218242
- TrueORF®
Mical1 (tGFP-tagged) - Mouse microtubule associated monoxygenase calponin and LIM domain containing 1 (Mical1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001164433" in other vectors (4)
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CNY 10,640.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MICAL; MICAL-1; Nical |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218242 representing NM_001164433
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATCACCCGCCTCCACCAACCCAGCACATGACCACTTTGAGACCTTTGTGCAGGCACAGCTGTGCC AGGATGTACTGAGTAGCTTTCAGGGGCTCTGTAGAGCCCTGGGAGTGGAGTCTGGTGGGGGATTGTCCCA GTACCACAAGATCAAGGCCCAGCTCAACTACTGGAGTGCCAAGTCACTGTGGGCCAAGTTGGACAAGAGA GCGAGCCAGCCTGTGTACCAGCAAGGCCAGGCCTGTACCAACACCAAGCTTCAGCTGCTTCTACTGAAGG TGGCCTTACTGTTGGGGGTGGAGATTCACTGGGGCGTCAAATTCACTGGCCTCCAGCCCCCTCCCAGAAA AGGGAGTGGCTGGCGTGCTCAGCTCCAGCCCAACCCCCCAGCCCAACTGGCCAGCTATGAGTTTGATGTC CTCATCTCAGCTGCAGGAGGCAAATTTGTCCCTGAAGGCTTCACTATACGAGAGATGCGTGGCAAACTGG CCATTGGCATCACAGCCAACTTTGTGAATGGGCGCACGGTGGAGGAGACACAGGTGCCGGAGATCAGTGG TGTAGCTCGAATCTACAACCAAAAATTCTTCCAGAGCCTGCTCAAAGCCACAGGTATTGATCTGGAGAAC ATTGTATACTACAAAGACGAGACCCACTACTTTGTGATGACAGCCAAGAAGCAGTGCCTGCTGAGGCTGG GGGTGCTGCGCCAGGACTTGTCAGAGACTGATCAGCTGCTGGGCAAAGCTAATGTGGTACCGGAGGCTCT GCAGCGCTTTGCCAGAGCAGCGGCTGACTTCGCCACACATGGCAAGCTTGGGAAACTGGAGTTTGCTCAG GATGCACGCGGGCGGCCTGATGTGGCGGCCTTCGACTTCACAAGCATGATGCGGGCAGAGAGTTCCGCTC GTGTCCAAGAAAAGCATGGTGCTCGCCTACTGCTGGGGCTGGTGGGGGACTGCCTAGTGGAGCCCTTCTG GCCTCTGGGCACTGGAGTAGCCCGAGGCTTCTTGGCAGCCTTCGATGCAGCCTGGATGGTGAAGCGGTGG GCAGAGGGCGCTGGGCCGCTAGAGGTGTTGGCTGAACGCGAGAGCTTGTACCAGCTTCTGTCACAAACAT CCCCCGAGAATATGCATCGAAATGTAGCCCAGTATGGGTTGGACCCTGCCACCCGATACCCCAACCTGAA CCTCCGGGCTGTAACCCCCAATCAGGTACAGGACCTCTATGATATGATGGACAAGGAGCATGCTCAGAGG AAGAGTGACGAGCCGGATTCCAGGAAGACAACCACAGGGTCCGCAGGCACGGAGGAGCTTCTGCACTGGT GCCAGGAGCAGACAGCTGGCTTTCCTGGAGTCCATGTCACTGACTTTTCTTCCTCGTGGGCTGATGGGCT AGCTCTGTGTGCCCTGGTACACCACCTACAGCCTGGCCTGCTGGAACCCTCGGAGCTGCAGGGCATGGGA GCTCTAGAAGCCACTACCTGGGCACTGAGGGTGGCAGAACATGAGCTAGGCATCACACCAGTGTTGTCTG CACAGGCAGTCATGGCTGGCAGTGACCCACTGGGTCTCATTGCCTACCTCAGCCACTTCCACAGTGCCTT CAAGAACACGAGCCACAGCTCAGGCCTTGTCAGCCAGCCCTCTGGAACCCCCAGTGCTATACTTTTCCTT GGCAAACTCCAGAGGAGCCTACAACGGACCCGCGCCAAGGTAGACGAGGAGACTCCGAGCACTGAGGAGC CGCCTGTCTCCGAGCCCAGTATGTCTCCAAACACTCCAGAGCTCTCTGAACACCAGGAGGCTGGGGCAGA GGAGCTGTGTGAACTCTGTGGGAAACATCTGTACATCCTAGAACGCTTCTGTGTGGATGGCCATTTCTTT CACCGGAGCTGCTTCTGCTGCCATACCTGTGAGGCCACATTGTGGCCAGGTGGCTATGGGCAACATCCAG GAGATGGACATTTCTACTGTCTCCAGCACCTACCCCAGGAGGACCAAAAGGAGGCTGACAACAATGGAAG TCTGGAGAGCCAGGAGCTCCCAACACCAGGAGACAGCAACATGCAGCCAGACCCCTCCTCTCCTCCTGTG ACGAGGGTCAGCCCTGTCCCAAGCCCCAGCCAGCCTGCACGTCGGCTGATCCGGCTCTCCAGTTTAGAAC GCCTACGGCTGTCCTCCCTGAATATCATCCCTGACTCAGGAGCGGAGCCTCCTCCCAAACCCCCAAGGAG CTGCTCAGACTTGGCCCGAGAATCCCTGAAGAGCAGCTTTGTGGGCTGGGGTGTGCCAGTCCAAGCACCA CAAGTTCCTGAAGCCATAGAGAAAGGGGATGATGAGGAGGAGGAAGAGGAAGAAGAGGAGGAGGAGGAGG AGCCACTGCCACCTTTGGAGCCAGAGTTGGAGCAGACTCTGCTGACCTTGGCCAAGAACCCAGGTGCCAT GACTAAGTACCCAACGTGGCGTCGAACCCTCATGCGCCGTGCCAAAGAGGAGGAGATGAAGAGGTTTTGC AAGGCCCAGGCCATCCAGCGAAGACTGAACGAGATTGAGGCCACTATGCGGGAGCTGGAGGCCGAAGGCA CGAAGCTGGAGCTGGCCTTGAGGAAAGAGAGTAGCTCTCCAGAACAGCAGAAGAAACTCTGGCTGGATCA GCTGCTACGGCTCATTCAGAAGAAGAACAGCCTAGTGACTGAAGAGGCCGAGCTCATGATCACGGTGCAG GAGCTGGATCTGGAAGAGAAGCAGCGGCAGCTAGACCACGAGTTGCGGGGCTACATGAATCGGGAAGAAA CTATGAAGACAGAGGCCGACCTGCAGTCTGAGAACCAGGTCCTAAGGAAGCTGTTGGAGGTGGTGAACCA GCGGGATGCTCTGATCCAATTCCAGGAGGAACGGAGGCTCAGAGAGATGCCTGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218242 representing NM_001164433
Red=Cloning site Green=Tags(s) MASPASTNPAHDHFETFVQAQLCQDVLSSFQGLCRALGVESGGGLSQYHKIKAQLNYWSAKSLWAKLDKR ASQPVYQQGQACTNTKLQLLLLKVALLLGVEIHWGVKFTGLQPPPRKGSGWRAQLQPNPPAQLASYEFDV LISAAGGKFVPEGFTIREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQKFFQSLLKATGIDLEN IVYYKDETHYFVMTAKKQCLLRLGVLRQDLSETDQLLGKANVVPEALQRFARAAADFATHGKLGKLEFAQ DARGRPDVAAFDFTSMMRAESSARVQEKHGARLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRW AEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPNQVQDLYDMMDKEHAQR KSDEPDSRKTTTGSAGTEELLHWCQEQTAGFPGVHVTDFSSSWADGLALCALVHHLQPGLLEPSELQGMG ALEATTWALRVAEHELGITPVLSAQAVMAGSDPLGLIAYLSHFHSAFKNTSHSSGLVSQPSGTPSAILFL GKLQRSLQRTRAKVDEETPSTEEPPVSEPSMSPNTPELSEHQEAGAEELCELCGKHLYILERFCVDGHFF HRSCFCCHTCEATLWPGGYGQHPGDGHFYCLQHLPQEDQKEADNNGSLESQELPTPGDSNMQPDPSSPPV TRVSPVPSPSQPARRLIRLSSLERLRLSSLNIIPDSGAEPPPKPPRSCSDLARESLKSSFVGWGVPVQAP QVPEAIEKGDDEEEEEEEEEEEEEPLPPLEPELEQTLLTLAKNPGAMTKYPTWRRTLMRRAKEEEMKRFC KAQAIQRRLNEIEATMRELEAEGTKLELALRKESSSPEQQKKLWLDQLLRLIQKKNSLVTEEAELMITVQ ELDLEEKQRQLDHELRGYMNREETMKTEADLQSENQVLRKLLEVVNQRDALIQFQEERRLREMPA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164433 |
| ORF Size | 2925 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164433.1, NP_001157905.1 |
| RefSeq Size | 3344 bp |
| RefSeq ORF | 2928 bp |
| Locus ID | 171580 |
| UniProt ID | Q8VDP3 |
| Gene Summary | Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preventing repolymerization. In the absence of actin, it also functions as a NADPH oxidase producing H(2)O(2) (By similarity). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Involved in regulation of lamina-specific connectivity in the nervous system such as the development of lamina-restricted hippocampal connections. Through redox regulation of the actin cytoskeleton controls the intracellular distribution of secretory vesicles containing L1/neurofascin/NgCAM family proteins in neurons, thereby regulating their cell surface levels. May act as Rab effector protein and play a role in vesicle trafficking.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222919 | Mical1 (untagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 2, (10ug) |
CNY 12,540.00 |
|
| MR218242 | Mical1 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 2 |
CNY 9,690.00 |
|
| MR218242L3 | Lenti ORF clone of Mical1 (Myc-DDK-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 2 |
CNY 11,590.00 |
|
| MR218242L4 | Lenti ORF clone of Mical1 (mGFP-tagged) - Mouse microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), transcript variant 2 |
CNY 11,590.00 |
