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Msh3 (NM_010829) Mouse Tagged ORF Clone
CAT＃: MG218117
- TrueORF®
Msh3 (tGFP-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3), (10ug)
ORF Plasmid: DDK
"NM_010829" in other vectors (4)
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CNY 10,550.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D13Em1; Rep-3; Rep3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218117 representing NM_010829
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCGGGAAATCCGCGTCGGGAGGCTCTACTGCCGCCGGCCCAGGCCCGGGGAGGCAAACGGTTC TAAGCCGGTTCTTCAGGTCTGCGGGAAGCCTAAGATCCAGCGCGTCCTCCACGGAGCCAGCAGAGAAGGT GACAGAAGGTGACAGCAGGAAGAGGTCACTGGGAAATGGTGGGCCCACTAAGAAAAAAGCAAGGAAAGTT CCAGAGAAGGAGGAAGAAAACATCTCAGTAGCATCTCACCACCCTGAGGCAAAGAAATGTCTGAGGCCCA GGATTGTTTTAAAGTCCCTGGAAAAGTTGAAAGAATTCTGCTGTGATTCTGCCCTCCCTCAAAACAGAGT CCAGACAGAAGCTCTTCGGGAGAGACTTGAAGTTCTGCCAAGGTGTACTGATTTTGAAGATATCACTCTG CAACGTGCAAAGAATGTGGTTTTGTCCGAAGATTCCAAATCTCAGGCTAATCAGAAAGACAGTCAATTTG GACCCTGCCCTGAAGTTTTCCAGAAGACTTCCGATTGTAAACCTTTTAACAAGCGATCCAAGAGCGTCTA TACACCGTTAGAACTGCAGTACCTAGATATGAAGCAGCAGCATAAAGACGCAGTTTTGTGCGTGGAGTGT GGTTACAAGTACAGATTCTTTGGGGAAGACGCAGAAATTGCTGCCCGGGAACTCAATATTTATTGCCACT TAGACCACAACTTTATGACTGCAAGCATACCTACCCACAGACTGTTCGTGCATGTCCGCCGACTTGTGGC CAAAGGGTACAAGGTGGGAGTTGTGAAGCAAACTGAAACTGCCGCATTAAAGGCCATCGGAGACAATAAA AGTTCTGTCTTCTCCCGGAAATTGACCGCTCTTTATACGAAATCCACACTTATTGGTGAAGATGTGAATC CTCTCATCAGGCTGGATGATTCTGTAAATATCGATGAGGTAATGACAGATATATCTACCAACTATCTTCT GTGTATCTACGAAGAAAAGGAGAACATTAAAGACAAAAAGAAGGGGAACCTTTCCGTTGGTGTCGTGGGA GTGCAGCCCGCAACTGGCGAGGTTGTGTTTGACTGCTTCCAGGACTCTGCTTCCAGACTGGAGCTGGAGA CTCGCATATCAAGCCTGCAGCCTGTGGAATTACTGCTTCCCTCCGACCTGTCTGTGCCAACAGAGATGCT CATCCAAAGAGCCACCAATGTCAGTGTGCGGGATGACAGGATTCGAGTAGAAAGGATGAATAATACTTAC TTTGAATACAGCCATGCTTTCCAGACAGTTACGGAGTTTTATGCTAGAGAGATAGTTGACAGCCAGGGCT CTCAGAGTCTCTCTGGTGTCATTAACTTGGAGAAGCCTGTGATCTGCGCCTTGGCTGCCGTAATAAGGTA CCTCAAGGAATTTAACCTGGAAAAGATGCTGTCCAAACCCGAGAGTTTTAAACAGCTGTCAAGTGGAATG GAATTCATGAGAATTAATGGGACCACGCTAAGGAATCTGGAGATCCTTCAGAATCAGACTGATATGAAGA CCAAAGGAAGCTTGCTTTGGGTTTTAGACCACACTAAAACTTCATTTGGGAGAAGGAAGTTAAAGAATTG GGTTACCCAGCCACTGCTTAAATTAAGGGAAATAAATGCCCGACTTGATGCAGTATCTGATGTTCTCCAC TCAGAATCCAGTGTGTTTGAGCAGATAGAAAACCTTCTTCGTAAGTTACCGGATGTCGAGAGAGGACTAT GTAGCATTTATCACAAGAAGTGCTCTACCCAAGAGTTCTTCTTGATCGTCAAAAGCCTATGTCAACTGAA GTCAGAGCTTCAAGCATTAATGCCTGCTGTTAATTCCCACGTTCAGTCCGACTTACTCCGAGCACTCATC GTGGAGGCTCCTGAGCTCCTCAGCCCAGTGGAACATTACTTAAAGGTTCTGAATGGGCCAGCTGCCAAAG TTGGCGATAAAACTGAATTATTCAAAGACCTCTCTGACTTCCCTTTAATAAAAAAGAGGAAAAATGAAAT TCAGGAAGTCATTCACAGCATCCAAATGCGTTTACAGGAATTTCGGAAAATATTAAAACTTCCTTCTTTA CAGTATGTGACTGTATCAGGACAAGAGTTTATGATTGAAATAAAGAACTCGGCTGTATCTTGCATCCCAG CTGATTGGGTGAAGGTTGGAAGCACAAAAGCTGTGAGCCGCTTTCACCCTCCCTTCATCGTGGAAAGCTA CAGACGTCTGAATCAGCTCCGGGAGCAGCTAGTCCTTGACTGCAATGCTGAATGGCTTGGCTTCCTAGAG AATTTTGGTGAACATTATCACACTTTGTGTAAGGCTGTGGATCACCTAGCAACTGTTGACTGTATTTTCT CCCTGGCCAAGGTCGCTAAACAAGGAAATTACTGCAGGCCAACTCTACAAGAAGAAAAGAAGATCATCAT AAAAAATGGAAGGCACCCTATGATTGATGTGTTGCTGGGTGAACAAGATCAGTTTGTGCCCAACAGTACG AGTTTATCACAGGACTCGGAGAGAGTAATGATAATCACCGGACCAAACATGGGTGGGAAAAGCTCCTACA TAAAACAGGTTGCACTGGTTACCATCATGGCTCAGATTGGCTCCTACGTCCCTGCAGAGGAAGCCACAAT TGGGATTGTGGATGGCATTTTCACAAGGATGGGTGCTGCAGACAATATATACAAAGGCCGGAGTACTTTC ATGGAAGAACTGACGGACACAGCGGAAATAATCAGGAGAGCGTCCCCACAGTCCTTGGTTATCCTGGATG AGCTGGGGAGAGGGACAAGCACCCATGACGGAATCGCCATCGCCTATGCAACTCTAGAGTATTTTATCAG AGACGTGAAATCCTTAACACTGTTCGTTACCCACTATCCACCAGTCTGTGAACTAGAAAAATGTTACCCA GAACAAGTGGGGAATTACCACATGGGATTCTTGGTCAATGAAGATGAAAGCAAGCAGGACTCAGGTGACA TGGAGCAGATGCCGGATTCTGTCACTTTTCTCTATCAGATAACCCGAGGAATTGCAGCGAGAAGTTATGG ACTTAATGTGGCGAAGTTAGCAGATGTACCTAGAGAAGTCTTACAGAAAGCCGCGCACAAGTCTAAGGAG CTGGAAGGCTTAGTCAGCCTGAGAAGGAAAAGGCTTGAATGTTTTACCGACTTATGGACGACACACAGTG TGAAGGACCTGCACACATGGGCAGATAAGCTGGAAATGGAGGAAATACAGACTTCTCTCCCACAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218117 representing NM_010829
Red=Cloning site Green=Tags(s) MPRGKSASGGSTAAGPGPGRQTVLSRFFRSAGSLRSSASSTEPAEKVTEGDSRKRSLGNGGPTKKKARKV PEKEEENISVASHHPEAKKCLRPRIVLKSLEKLKEFCCDSALPQNRVQTEALRERLEVLPRCTDFEDITL QRAKNVVLSEDSKSQANQKDSQFGPCPEVFQKTSDCKPFNKRSKSVYTPLELQYLDMKQQHKDAVLCVEC GYKYRFFGEDAEIAARELNIYCHLDHNFMTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNK SSVFSRKLTALYTKSTLIGEDVNPLIRLDDSVNIDEVMTDISTNYLLCIYEEKENIKDKKKGNLSVGVVG VQPATGEVVFDCFQDSASRLELETRISSLQPVELLLPSDLSVPTEMLIQRATNVSVRDDRIRVERMNNTY FEYSHAFQTVTEFYAREIVDSQGSQSLSGVINLEKPVICALAAVIRYLKEFNLEKMLSKPESFKQLSSGM EFMRINGTTLRNLEILQNQTDMKTKGSLLWVLDHTKTSFGRRKLKNWVTQPLLKLREINARLDAVSDVLH SESSVFEQIENLLRKLPDVERGLCSIYHKKCSTQEFFLIVKSLCQLKSELQALMPAVNSHVQSDLLRALI VEAPELLSPVEHYLKVLNGPAAKVGDKTELFKDLSDFPLIKKRKNEIQEVIHSIQMRLQEFRKILKLPSL QYVTVSGQEFMIEIKNSAVSCIPADWVKVGSTKAVSRFHPPFIVESYRRLNQLREQLVLDCNAEWLGFLE NFGEHYHTLCKAVDHLATVDCIFSLAKVAKQGNYCRPTLQEEKKIIIKNGRHPMIDVLLGEQDQFVPNST SLSQDSERVMIITGPNMGGKSSYIKQVALVTIMAQIGSYVPAEEATIGIVDGIFTRMGAADNIYKGRSTF MEELTDTAEIIRRASPQSLVILDELGRGTSTHDGIAIAYATLEYFIRDVKSLTLFVTHYPPVCELEKCYP EQVGNYHMGFLVNEDESKQDSGDMEQMPDSVTFLYQITRGIAARSYGLNVAKLADVPREVLQKAAHKSKE LEGLVSLRRKRLECFTDLWTTHSVKDLHTWADKLEMEEIQTSLPH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010829 |
| ORF Size | 3285 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010829.2, NP_034959.2 |
| RefSeq Size | 3945 bp |
| RefSeq ORF | 3288 bp |
| Locus ID | 17686 |
| UniProt ID | P13705 |
| Gene Summary | Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. MutS beta recognizes large insertion-deletion loops (IDL) up to 13 nucleotides long. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223548 | Msh3 (untagged) - Mouse mutS homolog 3 (E. coli) (Msh3), (10ug) |
CNY 14,060.00 |
|
| MR218117 | Msh3 (Myc-DDK-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3) |
CNY 7,624.00 |
|
| MR218117L3 | Lenti ORF clone of Msh3 (Myc-DDK-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3) |
CNY 11,500.00 |
|
| MR218117L4 | Lenti ORF clone of Msh3 (mGFP-tagged) - Mouse mutS homolog 3 (E. coli) (Msh3) |
CNY 11,500.00 |
