Rad17 (NM_011233) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MmRad24
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG210047 representing NM_011233 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGAAACTTTCCTTAGACCAAAGGTGTCTTCCACAAAGGTGACAGACTGGGTAGCTCCAGCCTTTG ATGACTTTGAGGCCAACACCGCCATCACCACCATCACTGCGTCATCTTTAACTTTCAGTAACTCCAGTCA CAGAAGAAAATATTTGCCCTCCACATTAGAAAGTAACAGATTGTCGGCTAGAAAAAGAGGAAGGCTCTCT TTAGAACAGACTCATGGTCTAGAAACTTCAAGAGAGCGTCTGTCCAACAATGAGCCGTGGGTGGATAAAT ATAAACCAGAAACTCAGCATGAACTTGCTGTTCATAAAAAGAAAATTGAAGAGGTCGAAACCTGGTTAAA AGCTCAAGTCTTAGAAGTGAAGCCAAAACAGGGTGGGTCTGTTTTATTAATAACAGGTCCTCCAGGATGT GGAAAGACGACTACTATAAAAATACTGTCAAAGGAACTTGGGATCCAAGTACAAGAATGGGTTAATCCTA TTTTACCAGACTTCCAAAAAGATGATTACAAGGAGTTACTTAGTTTAGAATCAAACTTCTCTGTAGTTCC ATACCAATCTCAGATAGCAGTCTTCAATGACTTTCTATTAAGAGCCACCAAGTACAGCAAACTGCAGATG CTTGGAGACGTTCTGACAACTGATAAGAAGATCATTCTGGTGGAAGAGTTACCTAACCAGTTTTATCGAG ACCCTAACGCTTTACATGAGATTCTAAGAAAGCATGTGCAGATTGGCCGGTGTCCTCTTGTATTTATAGT CTCTGACAGTGTCAGTGGAGACAATAATCAGCGGCTACTCTTCCCCAGAAACATTCAAGAAGAGTGTTCT GTTTCAAACATTAGTTTCAACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATTGTGACTA TAGAAGCTAGTAAGAATGGAGAAAAAATTATTGTTCCTAATAAAACTTCTCTGGAGTTGCTCTGTCAAGG ATGTTCTGGTGATATCAGAAGTGCAATAAACAGCCTGCAGTTCTCGTCTTCAAAAGGAGAGAACAGTTCG TGGTCAAAGAAGAAAAGAATGTCATTGAAATCGGATGCTGCCATATCAAAATCAAAACAAAAAAAGAAAC ATAATAGTACTTTGGAAAATCAAGAGATTCAAGCTATTGGTGGAAAAGATGTTTCTCTTTTTCTCTTCAA AGCTTTGGGGAAGATTCTGTACTGTAAAAGAGCGCCTTTAACAGAGTTAGACTCACCTCGCCTGCCTGCT CACTTGTCAGAGCACGACCGGGACACTTTACTTGTTCAGCCTGAGGAAATAGTAGAAATGTCACACATGT CAGGAGACTTTTTTAATTTATACCTTCACCAAAACTACATAGATTTCTTCGCTGAAGTAGATGATCTTGT GCCAGCCAGTGAGTTTCTGAGTTTTGCAGATATCCTCGGTGGTGATTGGAATACACGCTCGTTACTCAGG GAATATAGTACATCCGTAGCTACAAGAGGTGTGATGCATTCCAACAAAGCCCGGGGGTTCGCTCACTGCC AAGGAGGATCAAGCTTTCGACCCTTACATAAGCCCCAGTGGTTTCTAATACAGAAAAAGTATCGGGAAAA TTGCCTGGCAGCAAAAGCACTCTTTGTTGACTTCTGCCTACCAGCTTTGTGTCTCCAGACTCAGCTCTTG CCATATCTTGCTCTTCTGACCATTCCAATGCGAAATAAAGCTCAGATTTCTTTTATCCAAGATGTTGGAA GACTTCCTTTGAAGCGAAGCTTTGGAAGGTTGAAAATGGAAGCCCTGACTGACCGAGAGCTGGGGCTGAT AGACCCTGACAGTGGGGATGAGTCCCCGCACAGCGGAGGGCAGCCGGCTCAGGAAGCTCCCGGTGAACCC GCGCAGGCTGCTCAGAATGCCGACCCAGAGACCTGGTCCCTTCCGCTGAGTCAGAACAGCGGGAGCGATC TGCCTGCCAGCCAGCCTCAGCCCTTTTCCTCCAAAGTGGACATGGAAGAAGAGGAGGAGGAAGAGGAAGA CATAATAATAGAGGACTATGACAGCGAGGAGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG210047 representing NM_011233 Red=Cloning site Green=Tags(s) MSETFLRPKVSSTKVTDWVAPAFDDFEANTAITTITASSLTFSNSSHRRKYLPSTLESNRLSARKRGRLS LEQTHGLETSRERLSNNEPWVDKYKPETQHELAVHKKKIEEVETWLKAQVLEVKPKQGGSVLLITGPPGC GKTTTIKILSKELGIQVQEWVNPILPDFQKDDYKELLSLESNFSVVPYQSQIAVFNDFLLRATKYSKLQM LGDVLTTDKKIILVEELPNQFYRDPNALHEILRKHVQIGRCPLVFIVSDSVSGDNNQRLLFPRNIQEECS VSNISFNPVAPTIMMKFLNRIVTIEASKNGEKIIVPNKTSLELLCQGCSGDIRSAINSLQFSSSKGENSS WSKKKRMSLKSDAAISKSKQKKKHNSTLENQEIQAIGGKDVSLFLFKALGKILYCKRAPLTELDSPRLPA HLSEHDRDTLLVQPEEIVEMSHMSGDFFNLYLHQNYIDFFAEVDDLVPASEFLSFADILGGDWNTRSLLR EYSTSVATRGVMHSNKARGFAHCQGGSSFRPLHKPQWFLIQKKYRENCLAAKALFVDFCLPALCLQTQLL PYLALLTIPMRNKAQISFIQDVGRLPLKRSFGRLKMEALTDRELGLIDPDSGDESPHSGGQPAQEAPGEP AQAAQNADPETWSLPLSQNSGSDLPASQPQPFSSKVDMEEEEEEEEDIIIEDYDSEET TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_011233
ORF Size	2860 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_011233.2
RefSeq Size	2856 bp
RefSeq ORF	2067 bp
Locus ID	19356
UniProt ID	Q6NXW6
Gene Summary	Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination (By similarity). Essential for embryonic development. May be involved in homologous recombination.[UniProtKB/Swiss-Prot Function]

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