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Txnrd1 (NM_015762) Mouse Tagged ORF Clone
CAT＃: MG208008
- TrueORF®
Txnrd1 (tGFP-tagged) - Mouse thioredoxin reductase 1 (Txnrd1), transcript variant 3
"NM_015762" in other vectors (2)
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CNY 3,710.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | T; TR; TR1; Trx; TrxR1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208008 representing NM_015762
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGCTCCAAAGATCCCCCTGGGTCCTATGACTTCGACCTGATTATCATTGGAGGAGGCTCAGGAG GACTGGCAGCAGCTAAGGAGGCAGCCAAATTTGACAAGAAAGTGCTGGTCTTGGATTTTGTCACACCGAC TCCTCTTGGGACCAGATGGGGTCTCGGAGGAACGTGTGTGAATGTGGGTTGCATACCTAAGAAGCTGATG CACCAGGCAGCTTTGCTCGGACAAGCTCTGAAAGACTCGCGCAACTATGGCTGGAAAGTCGAAGACACAG TGAAGCATGACTGGGAGAAAATGACGGAATCTGTGCAGAGTCACATCGGCTCGCTGAACTGGGGCTACCG CGTAGCTCTCCGGGAGAAAAAGGTCGTCTATGAGAATGCTTACGGGAGGTTCATTGGTCCTCACAGGATT GTGGCGACAAATAACAAAGGTAAAGAAAAAATCTATTCAGCAGAGCGGTTCCTCATCGCCACAGGTGAGA GGCCCCGCTACCTGGGCATCCCTGGAGACAAAGAGTACTGCATCAGCAGTGATGATCTTTTCTCCTTGCC TTACTGCCCGGGGAAGACCCTAGTAGTTGGTGCATCCTATGTCGCCTTGGAATGTGCAGGATTTCTGGCT GGTATCGGCTTAGACGTCACTGTAATGGTGCGGTCCATTCTCCTTAGAGGATTTGACCAAGACATGGCCA ACAAAATCGGTGAACACATGGAAGAACATGGTATCAAGTTTATAAGGCAGTTCGTCCCAACGAAAATTGA ACAGATCGAAGCAGGAACACCAGGCCGACTCAGGGTGACTGCTCAATCCACAAACAGCGAGGAGACCATA GAGGGCGAATTTAACACAGTGTTGCTGGCGGTAGGAAGAGATTCTTGTACGAGAACTATTGGCTTAGAGA CCGTGGGCGTGAAGATAAACGAAAAAACCGGAAAGATACCCGTCACGGATGAAGAGCAGACCAATGTGCC TTACATCTACGCCATCGGTGACATCCTGGAGGGGAAGCTAGAGCTGACTCCCGTAGCCATCCAGGCGGGG AGATTGCTGGCTCAGAGGCTGTACGGAGGCTCCAATGTCAAATGTGACTATGACAATGTCCCAACGACTG TATTTACTCCTTTGGAATATGGCTGTTGTGGCCTCTCTGAAGAAAAAGCCGTAGAGAAATTTGGGGAAGA AAATATTGAAGTTTACCATAGTTTCTTTTGGCCATTGGAATGGACAGTCCCATCCCGGGATAACAACAAA TGTTATGCAAAAATAATCTGCAACCTTAAAGACGATGAACGTGTCGTGGGCTTCCACGTGCTGGGTCCAA ACGCTGGAGAGGTGACGCAGGGCTTTGCGGCTGCGCTCAAGTGTGGGCTGACTAAGCAGCAGCTGGACAG CACCATCGGCATCCACCCGGTCTGTGCAGAGATATTCACAACGTTGTCAGTGACGAAGCGCTCTGGGGGA GACATCCTCCAGTCTGGCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208008 representing NM_015762
Red=Cloning site Green=Tags(s) MNGSKDPPGSYDFDLIIIGGGSGGLAAAKEAAKFDKKVLVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLM HQAALLGQALKDSRNYGWKVEDTVKHDWEKMTESVQSHIGSLNWGYRVALREKKVVYENAYGRFIGPHRI VATNNKGKEKIYSAERFLIATGERPRYLGIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLA GIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGIKFIRQFVPTKIEQIEAGTPGRLRVTAQSTNSEETI EGEFNTVLLAVGRDSCTRTIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAG RLLAQRLYGGSNVKCDYDNVPTTVFTPLEYGCCGLSEEKAVEKFGEENIEVYHSFFWPLEWTVPSRDNNK CYAKIICNLKDDERVVGFHVLGPNAGEVTQGFAAALKCGLTKQQLDSTIGIHPVCAEIFTTLSVTKRSGG DILQSGC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015762 |
| ORF Size | 1500 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015762.2, NP_056577.2 |
| RefSeq Size | 3310 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 50493 |
| UniProt ID | Q9JMH6 |
| Gene Summary | The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms. [provided by RefSeq, May 2017] |
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