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Kdm8 (NM_029842) Mouse Tagged ORF Clone
CAT＃: MG206539
- TrueORF®
Kdm8 (tGFP-tagged) - Mouse jumonji domain containing 5 (Jmjd5)
ORF Plasmid: DDK
"NM_029842" in other vectors (4)
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CNY 3,140.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3110005O21Rik; Jmjd5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206539 representing NM_029842
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGAGGACACCACAGAGCCCCTGGTGGGGTCCAGCACCCTCTGGAAGGAGCTCAGGACGCTCCTGC CTGACAAAGAGGAGGAGCTGAAGCTGGACCTCGGTGAGAAGGTGGACAGGAGCGTGGCTGCGCTTTTGCG GCAGGCTGTGGGTCTATTCTACGCGGGCCATTGGCAGGGGTGCCTGCAGGCCAGTGAGGCCGTCCTAGAC TACTCCTGGGAAAAGCTCAACACTGGTCCCTGGAGAGATGTAGACAAGGAGTGGCGCCGGGTATATTCCT TCGGCTGCCTCCTGAAGGCCCTGTGCCTGTGCCAGGCACCACAGAAGGCCACTACTGTGGTGGAAGCACT GCGGGTGTGTGACATGGGCCTGTTGATGGGGGCAGCCATCCTCGAGGACATTCTTCTCAAAGTTGTCGCA GTCCTCCAGACACACCAGCTCCCAGGAAAGCAGCCTGCCCGCGGCCCCCACCAGGATCAGCCTGCCACCA AGAAGGCAAAGTGTGACGCCAGCCCAGCTCCTGATGTCATGTTAGAGCGGATGGTACCCAGGCTCCGCTG CCCACCTCTGCAGTACTTCAAGCAGCATTTTCTAGTTCCTGGGAGGCCTGTGATCTTGGAAGGTGTGGCC GACCACTGGCCGTGCATGAAGAAGTGGAGTCTGCAGTACATCCAGGAGATTGCTGGCTGCCGCACCGTCC CCGTGGAAGTGGGCTCAAGGTACACAGATGAAGACTGGTCCCAGACTCTCATGACAGTCGATGAGTTCAT CCAGAAGTTCATCCTGAGCGAGGCAAAGGATGTCGGGTACCTTGCTCAGCACCAGCTCTTCGACCAGATC CCAGAGTTGAAGCGGGACATCAGCATCCCTGATTACTGTTGCCTGGGCAATGGAGAAGAGGAAGAGATCA CTATCAATGCCTGGTTTGGTCCACAAGGCACCATCTCCCCACTGCATCAGGACCCCCAGCAGAACTTCCT GGTCCAGGTGCTAGGAAGGAAGTACATCCGATTGTATTCGCCGCAAGAGTCTGAGGCAGTGTACCCTCAC GAGACGCACATTCTTCATAACACCAGCCAGGTTGATGTGGAAAACCCCGACCTTGAGAAGTTTCCCAAGT TCACTGAGGCCCCATTCCTGTCCTGCATTCTGTCCCCAGGAGACACCCTCTTTATACCTGCTAAGTACTG GCATTATGTGCGCTCCCTGGACCTGAGCTTCTCTGTCAGCTTCTGGTGGTCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206539 representing NM_029842
Red=Cloning site Green=Tags(s) MSEDTTEPLVGSSTLWKELRTLLPDKEEELKLDLGEKVDRSVAALLRQAVGLFYAGHWQGCLQASEAVLD YSWEKLNTGPWRDVDKEWRRVYSFGCLLKALCLCQAPQKATTVVEALRVCDMGLLMGAAILEDILLKVVA VLQTHQLPGKQPARGPHQDQPATKKAKCDASPAPDVMLERMVPRLRCPPLQYFKQHFLVPGRPVILEGVA DHWPCMKKWSLQYIQEIAGCRTVPVEVGSRYTDEDWSQTLMTVDEFIQKFILSEAKDVGYLAQHQLFDQI PELKRDISIPDYCCLGNGEEEEITINAWFGPQGTISPLHQDPQQNFLVQVLGRKYIRLYSPQESEAVYPH ETHILHNTSQVDVENPDLEKFPKFTEAPFLSCILSPGDTLFIPAKYWHYVRSLDLSFSVSFWWS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029842 |
| ORF Size | 1242 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029842.5 |
| RefSeq Size | 2379 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 77035 |
| UniProt ID | Q9CXT6 |
| Gene Summary | Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase. Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation. Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity. Upon DNA damage, cleaves the N-terminal tail of histone H3 at monomethylated lysine residues, preferably at monomethylated 'Lys-9' (H3K9me1). The histone variant H3F3A is the major target for cleavage. Additionnally, acts as Fe(2+) and 2-oxoglutarate-dependent monoxygenase, catalyzing (R)-stereospecific hydroxylation at C-3 of 'Arg-137' of RPS6 and 'Arg-141' of RCCD1, but the biological significance of this activity remains to be established. Regulates mitosis through different mechanisms: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1. Possibly together with RCCD1, is involved in proper mitotic spindle organization and chromosome segregation. Negatively regulates cell cycle repressor CDKN1A/p21, which controls G1/S phase transition. Required for G2/M phase cell cycle progression. Regulates expression of CCNA1/cyclin-A1, leading to cancer cell proliferation. Also, plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability involved in epithelial to mesenchymal transition (By similarity). Regulates the circadian gene expression in the liver (PubMed:30500822). Represses the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a catalytically-independent manner (By similarity). Negatively regulates the protein stability and function of CRY1; required for AMPK-FBXL3-induced CRY1 degradation (PubMed:30500822).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC204131 | Kdm8 (untagged) - Mouse jumonji domain containing 5 (Jmjd5), (10ug) |
CNY 2,000.00 |
|
| MR206539 | Kdm8 (Myc-DDK-tagged) - Mouse jumonji domain containing 5 (Jmjd5) |
CNY 2,850.00 |
|
| MR206539L3 | Lenti ORF clone of Kdm8 (Myc-DDK-tagged) - Mouse jumonji domain containing 5 (Jmjd5) |
CNY 4,750.00 |
|
| MR206539L4 | Lenti ORF clone of Kdm8 (mGFP-tagged) - Mouse jumonji domain containing 5 (Jmjd5) |
CNY 4,750.00 |
