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Aurkb (NM_011496) Mouse Tagged ORF Clone
CAT＃: MG205201
- TrueORF®
Aurkb (tGFP-tagged) - Mouse aurora kinase B (Aurkb)
ORF Plasmid: DDK
"NM_011496" in other vectors (4)
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CNY 3,140.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI; Aik2; AIM-1; Aim1; AIRK2; AL022959; Ark2; AurB; I; IPL1; Stk; STK-; STK-1; Stk1; Stk5; Stk12 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205201 representing NM_011496
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGAAGGAGAACGCCTACCCGTGGCCCTACGGCTCAAAGACGTCTCAGTCTGGCCTGAACACGT TGTCCCAGAGAGTCCTACGGAAGGAGCCCGCCACGACATCTGCGCTTGCTCTCGTGAACCGGTCCAACAG CCAGTCCACAGCTGCCCCTGGCCAGAAGTTGGCTGAGAACAAGAGTCAGGGCTCCACTGCCTCGCAAGGA TCCCAGAACAAGCAGCCTTTCACTATTGACAACTTTGAGATTGGGCGTCCTTTGGGCAAAGGCAAATTTG GAAACGTGTACTTGGCTCGGGAGAAGAAGAGCCGTTTCATCGTGGCACTCAAGATCCTCTTCAAGTCTCA GATTGAGAAGGAGGGGGTAGAGCACCAGCTTCGCCGAGAGATCGAAATCCAGGCGCACCTGAAACATCCC AACATCCTTCAACTCTACAACTACTTCTACGACCAGCAGAGGATCTACTTAATCCTGGAATACGCCCCTC GCGGGGAACTCTACAAGGAACTGCAGAAGAGTCGGACCTTCGATGAGCAGCGGACTGCCACGATCATGGA GGAACTGTCAGATGCCCTGACCTACTGCCACAAGAAGAAGGTAATTCACAGAGACATAAAGCCGGAGAAC CTGCTGTTAGGTCTGCAGGGAGAACTGAAGATTGCAGACTTTGGCTGGTCGGTGCATGCCCCATCCCTGA GGAGGAAGACCATGTGCGGCACGCTGGACTATCTGCCCCCAGAGATGATTGAGGGGCGCATGCATAATGA AATGGTAGATCTATGGTGCATCGGGGTGCTCTGCTATGAACTGATGGTGGGGAACCCACCCTTCGAGAGC CCTAGCCACAGTGAGACGTATCGTCGGATTGTCAAGGTGGACCTGAAGTTCCCCTCTTCTGTGCCTTCGG GCGCCCAGGACCTCATCTCCAAGCTGCTCAAACATAACCCCTGGCAACGGCTGCCCCTGGCGGAGGTTGC AGCTCACCCTTGGGTCCGGGCCAACTCAAGGAGGGTTCTGCCTCCCTCTGCCCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205201 representing NM_011496
Red=Cloning site Green=Tags(s) MAQKENAYPWPYGSKTSQSGLNTLSQRVLRKEPATTSALALVNRSNSQSTAAPGQKLAENKSQGSTASQG SQNKQPFTIDNFEIGRPLGKGKFGNVYLAREKKSRFIVALKILFKSQIEKEGVEHQLRREIEIQAHLKHP NILQLYNYFYDQQRIYLILEYAPRGELYKELQKSRTFDEQRTATIMEELSDALTYCHKKKVIHRDIKPEN LLLGLQGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEMVDLWCIGVLCYELMVGNPPFES PSHSETYRRIVKVDLKFPSSVPSGAQDLISKLLKHNPWQRLPLAEVAAHPWVRANSRRVLPPSAL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011496 |
| ORF Size | 1035 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011496.1, NP_035626.1 |
| RefSeq Size | 1957 bp |
| RefSeq ORF | 1038 bp |
| Locus ID | 20877 |
| UniProt ID | O70126 |
| Gene Summary | This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 2 and 7. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207410 | Aurkb (untagged) - Mouse aurora kinase B (Aurkb), (10ug) |
CNY 3,990.00 |
|
| MR205201 | Aurkb (Myc-DDK-tagged) - Mouse aurora kinase B (Aurkb) |
CNY 2,850.00 |
|
| MR205201L3 | Lenti ORF clone of Aurkb (Myc-DDK-tagged) - Mouse aurora kinase B (Aurkb) |
CNY 4,750.00 |
|
| MR205201L4 | Lenti ORF clone of Aurkb (mGFP-tagged) - Mouse aurora kinase B (Aurkb) |
CNY 4,750.00 |
