United States
Germany
Japan
United Kingdom
China
Bcdin3d (NM_029236) Mouse Tagged ORF Clone
CAT＃: MG203874
- TrueORF®
Bcdin3d (tGFP-tagged) - Mouse RIKEN cDNA 4930556P03 gene (4930556P03Rik)
ORF Plasmid: DDK
"NM_029236" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930556P03Rik; AV138748 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203874 representing NM_029236
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGACGGGACATTGTCAAGAGGGGGCGTTGGAGAGGCCGTGGAGGAAGAGCATCCCGGGGCTC TGGAACCCGGGGCCGCCCCGTTCGGAAATTTCCCTCATTATTCCCGCTTCCACCCTCCCGAGCAAAGGCT CCGCCTTCTGCCCCCGGAGCTTCTTCGGCAGCTCTTCCCTCCCGAGGGTCCCGAGAAGAGGCCGATTCTA GGGCTCGACGTGGGGTGTAACTCCGGGGATCTGAGTGTGGCTCTGTACAAACATTTCCTTTCCCCTCGCG ATGGGGAGACCTGCTCCGGTGCATCCAGAGAACTCCGCATCCTCTGCTGTGATATAGATCCAGTCCTTGT GGAGAGGGCTGAAAGAGACTGTCCCTTCCCTGAGGCTTTGACCTTTATCACCCTGGACATCATGGATCAA GAGAGCAGGAAGGTTCCCTTGAGTTCTTTCTTGAGCCAGTTTGGGCGTTCCGTTTTTGACATGGTCTTCT GCATGTCAGTAACCATGTGGATTCATCTGAACCACGGGGACCGTGGTCTGTGCGAGTTCCTGGCCCACGT CTCCTCTCTCTGCAGCTACCTCCTCGTGGAGCCACAACCCTGGAAGTGTTACCGGGCAGCTGCAAGGCGC CTGCGCAAGCTGGGACTCCACAGTTTTGATCACTTCCGCTCGCTGGCCATCCGAGGTGACATGGCCAAGC AGATCGTGCGGATCTTGACGCAGGACCACGGGATGGAGTTAGCGTGCTGTTTCGGCAACACCAGTTGGGA CCGAAGCCTTCTGCTCTTCAGAGCAAAGCACACCCACGAGACTCAGGCAATCCCCGAATCGTCAACAAAA GAGACACGGACAGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203874 representing NM_029236
Red=Cloning site Green=Tags(s) MAADGTLSRGGVGEAVEEEHPGALEPGAAPFGNFPHYSRFHPPEQRLRLLPPELLRQLFPPEGPEKRPIL GLDVGCNSGDLSVALYKHFLSPRDGETCSGASRELRILCCDIDPVLVERAERDCPFPEALTFITLDIMDQ ESRKVPLSSFLSQFGRSVFDMVFCMSVTMWIHLNHGDRGLCEFLAHVSSLCSYLLVEPQPWKCYRAAARR LRKLGLHSFDHFRSLAIRGDMAKQIVRILTQDHGMELACCFGNTSWDRSLLLFRAKHTHETQAIPESSTK ETRTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029236 |
| ORF Size | 855 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029236.1, NP_083512.1 |
| RefSeq Size | 1273 bp |
| RefSeq ORF | 858 bp |
| Locus ID | 75284 |
| UniProt ID | Q91YP1 |
| Gene Summary | O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA, acting as a capping enzyme. Less efficiently, also methylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre-miRNAs. There is some controversy about the methylation of pre-miR-145, since the dimethylation first described as the specific enzymatic activity cannot be reproduced by a more recent work which observes a monomehtylation of pre-miR-145 but two orders weaker than the methylation of cytosolic histidyl tRNA.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR203874 | Bcdin3d (Myc-DDK-tagged) - Mouse BCDIN3 domain containing (Bcdin3d) |
CNY 1,416.00 |
|
| MR203874L3 | Lenti ORF clone of Bcdin3d (Myc-DDK-tagged) - Mouse BCDIN3 domain containing (Bcdin3d) |
CNY 4,750.00 |
|
| MR203874L4 | Lenti ORF clone of Bcdin3d (mGFP-tagged) - Mouse BCDIN3 domain containing (Bcdin3d) |
CNY 4,750.00 |
其它Bcdin3d产品
