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Rps3 (NM_012052) Mouse Tagged ORF Clone
CAT＃: MG203024
- TrueORF®
Rps3 (tGFP-tagged) - Mouse ribosomal protein S3 (Rps3)
ORF Plasmid: DDK
"NM_012052" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D7Ertd795e; Rs_3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203024 representing NM_012052
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGCAGATTTCCAAGAAGAGGAAGTTTGTAGCTGATGGCATCTTCAAAGCTGAGCTGAATGAAT TTCTCACTCGGGAGCTGGCTGAAGATGGCTACTCTGGAGTTGAAGTCCGAGTTACACCAACCAGGACAGA AATCATTATTTTAGCCACCAGGACACAGAATGTTCTTGGGGAGAAGGGTCGTCGGATCAGAGAGTTGACC GCAGTTGTCCAGAAGCGCTTTGGCTTCCCTGAAGGCAGCGTAGAGCTTTATGCAGAGAAAGTGGCCACAA GAGGTCTGTGTGCCATTGCCCAGGCAGAGTCTCTACGCTACAAACTCCTTGGAGGGCTTGCGGTTCGAAG GGCCTGCTATGGTGTGCTTCGGTTCATTATGGAGAGTGGGGCCAAGGGCTGCGAGGTTGTGGTGTCTGGG AAGCTCCGAGGACAGAGGGCCAAGTCCATGAAGTTTGTGGATGGTCTGATGATTCACAGTGGAGACCCTG TTAACTACTATGTCGACACAGCCGTGCGCCATGTGCTCCTCAGACAGGGTGTGCTGGGCATCAAAGTGAA GATCATGCTGCCCTGGGACCCAAGTGGTAAGATTGGTCCCAAGAAGCCTCTGCCTGATCATGTGAGCATC GTGGAACCTAAAGATGAAATCCTGCCCACGACCCCCATCTCCGAACAGAAGGGTGGGAAGCCAGAGCCAC CAGCCATGCCCCAGCCAGTGCCTACAGCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203024 representing NM_012052
Red=Cloning site Green=Tags(s) MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLGEKGRRIRELT AVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSG KLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPSGKIGPKKPLPDHVSI VEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012052 |
| ORF Size | 729 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012052.1 |
| RefSeq Size | 1879 bp |
| RefSeq ORF | 732 bp |
| Locus ID | 27050 |
| UniProt ID | P62908 |
| Gene Summary | Involved in translation as a component of the 40S small ribosomal subunit (By similarity). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (By similarity). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (By similarity). Has also been shown to bind with similar affinity to intact and damaged DNA (By similarity). Stimulates the N-glycosylase activity of the base excision protein OGG1 (By similarity). Enhances the uracil excision activity of UNG1 (By similarity). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (By similarity). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (By similarity). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (By similarity). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (By similarity). Represses its own translation by binding to its cognate mRNA (By similarity). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (By similarity). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (By similarity). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (By similarity). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC207469 | Rps3 (untagged) - Mouse ribosomal protein S3 (Rps3), (10ug) |
CNY 3,990.00 |
|
| MR203024 | Rps3 (Myc-DDK-tagged) - Mouse ribosomal protein S3 (Rps3) |
CNY 1,416.00 |
|
| MR203024L3 | Lenti ORF clone of Rps3 (Myc-DDK-tagged) - Mouse ribosomal protein S3 (Rps3) |
CNY 4,750.00 |
|
| MR203024L4 | Lenti ORF clone of Rps3 (mGFP-tagged) - Mouse ribosomal protein S3 (Rps3) |
CNY 4,750.00 |
