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Gsta1 (NM_008181) Mouse Tagged ORF Clone
CAT＃: MG202575
- TrueORF®
Gsta1 (tGFP-tagged) - Mouse glutathione S-transferase, alpha 1 (Ya) (Gsta1)
ORF Plasmid: DDK
"NM_008181" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Gst2-1; OTTMUSG00000031890 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG202575 representing NM_008181
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGAAGCCCGTGCTTCACTACTTCAATGCCCGGGGCAGAATGGAGTGCATCAGGTGGCTCCTGG CTGCAGCAGGGGTGGAGTTTGAAGAGAAGTTTATACAGAGTCCGGAAGATTTGGAAAAGCTAAAAAAAGA TGGGAATTTGATGTTTGACCAAGTGCCCATGGTGGAGATTGATGGGATGAAGCTGGCGCAGACCAGAGCC ATTCTCAACTACATCGCCACCAAATATGACCTCTATGGGAAGGACATGAAGGAGAGAGCCCTGATTGACA TGTATTCAGAAGGTATTTTAGATCTGACTGAAATGATTGGGCAATTGGTATTATGTCCCCCAGACCAAAG AGAAGCCAAGACTGCCTTGGCAAAAGATAGGACCAAAAACCGTTACTTGCCTGCCTTTGAAAAGGTGTTG AAGAGCCATGGACAAGACTACCTTGTGGGCAACAGGCTGACCAGGGTGGACATCCACCTACTGGAAGTTC TCCTCTATGTTGAAGAGTTTGATGCCAGCCTTCTGACCCCTTTCCCTCTGCTGAAGGCCTTCAAGAGCAG AATCAGCAGCCTCCCCAATGTGAAGAAGTTCCTACAGCCTGGCAGCCAGAGAAAGCCTCCCATGGATGCA AAACAAATTCAAGAAGCAAGGAAGGCTTTCAAGATTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG202575 representing NM_008181
Red=Cloning site Green=Tags(s) MAGKPVLHYFNARGRMECIRWLLAAAGVEFEEKFIQSPEDLEKLKKDGNLMFDQVPMVEIDGMKLAQTRA ILNYIATKYDLYGKDMKERALIDMYSEGILDLTEMIGQLVLCPPDQREAKTALAKDRTKNRYLPAFEKVL KSHGQDYLVGNRLTRVDIHLLEVLLYVEEFDASLLTPFPLLKAFKSRISSLPNVKKFLQPGSQRKPPMDA KQIQEARKAFKIQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008181 |
| ORF Size | 669 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008181.3, NP_032207.3 |
| RefSeq Size | 861 bp |
| RefSeq ORF | 672 bp |
| Locus ID | 14857 |
| UniProt ID | P13745 |
| Gene Summary | Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208604 | Gsta1 (untagged) - Mouse glutathione S-transferase, alpha 1 (Ya) (Gsta1), (10ug) |
CNY 2,400.00 |
|
| MR202575 | Gsta1 (Myc-DDK-tagged) - Mouse glutathione S-transferase, alpha 1 (Ya) (Gsta1) |
CNY 2,081.00 |
|
| MR202575L3 | Lenti ORF clone of Gsta1 (Myc-DDK-tagged) - Mouse glutathione S-transferase, alpha 1 (Ya) (Gsta1) |
CNY 4,750.00 |
|
| MR202575L4 | Lenti ORF clone of Gsta1 (mGFP-tagged) - Mouse glutathione S-transferase, alpha 1 (Ya) (Gsta1) |
CNY 4,750.00 |
