Villin (VIL1) Mouse Monoclonal Antibody [Clone ID: CWWB1]

Specifications

Product Data
Clone Name	CWWB1
Applications	IHC
Recommend Dilution	IHC: 1:100 - 1:200 WB: 1:500 - 1:1000
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Prokaryotic recombinant protein corresponding to the C-terminal "headpiece” region of the human villin molecule
Specificity	Human villin protein
Formulation	Liquid tissue culture supernatant containing 15 mM sodium azide as a preservative
Conjugation	Unconjugated
Storage Condition	Store at 2-8°C
Gene Name	villin 1
Database Link	Entrez Gene 7429 Human UniProt ID P09327
Background	Villin and the structurally-related proteins gelsolin, fragmin and severin, all regulate the framework and assembly of actin. Villin is unique among these proteins in its ability to cross-link actin filaments into bundles, a process observed only at low Ca2+ concentration. Villin is composed of three domains. The first two domains are homologous and the third domain is called the headpiece. This headpiece region is located at the C-terminus. Villin is mainly produced by epithelial cells that develop a brush border. Cells producing villin are reported to be found either in the epithelial cells of the intestinal mucosa and gallbladder, or in epithelial cells of the kidney proximal tubules and ductuli efferentes of the testis. However, villin is also reported to be found in some epithelia which lack a brush border but which are derived from embryonic gut such as duct cells of the exocrine pancreas and biliary cells of the liver. In these cell types, villin is concentrated in the apical cytoplasm. Epithelial cells of the intestinal mucosa are continually being renewed and this involves a migration of these cell types from the intestinal crypts to the tips of the villi, gradually acquiring their differentiated phenotype as they do so. The maximum production of villin occurs at the base of the villus. Villin, therefore, shows tissue-specific expression being restricted to certain epithelia and their apical domains, thus indicating their polarity. The morphological loss of polarity of colonic epithelial cells is reported to be one of the most significant indicators of dysplasia or neoplasia.
Synonyms	D2S1471; VIL
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.