p21 (CDKN1A) pSer130 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC
Recommend Dilution	ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50~1/100.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S130 of human p21Cip1.
Specificity	This antibody detects p21Cip1 pSer130.
Formulation	PBS with 0.09% (W/V) Sodium Azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein A Chromatography, followed by two-step phosphospecific peptide affinity purification.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cyclin-dependent kinase inhibitor 1A
Database Link	Entrez Gene 1026 Human UniProt ID P38936
Background	p21 is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this protein is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. p21 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. It was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.
Synonyms	CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1
Note	Molecular weight: 17988 Da
Reference Data

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