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GK2 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
GK2 (Myc-DDK-tagged)-Human glycerol kinase 2 (GK2)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
GK2 (tGFP-tagged) - Human glycerol kinase 2 (GK2)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti-ORF clone of GK2 (Myc-DDK-tagged)-Human glycerol kinase 2 (GK2)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti-ORF clone of GK2 (mGFP-tagged)-Human glycerol kinase 2 (GK2)
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
GK2 CRISPRa kit - CRISPR gene activation of human glycerol kinase 2
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
GK2 (untagged)-Human glycerol kinase 2 (GK2)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
qPCR primer pairs and template standards against Homo sapiens gene GK2
| Application | Plasmid of exact quantity for transcript copy number calculation |
GK2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
GK2 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
3' UTR clone of glycerol kinase 2 (GK2) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene GK2
GK2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
