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GK2 (NM_033214) Human Tagged ORF Clone
CAT＃: RG220661
- TrueORF®
GK2 (tGFP-tagged) - Human glycerol kinase 2 (GK2)
ORF Plasmid: DDK
"NM_033214" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GKP2; GKTA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG220661 representing NM_033214
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCCCAAAGACAGCAGCTGTGGGGCCGTTGGTGGGAGCGGTGGTCCAGGGCACCAACTCCACTC GCTTTCTGGTTTTCAATTCAAAAACAGCGGAACTACTTAGTCATCACAAAGTGGAATTAACACAAGAGTT CCCAAAAGAAGGATGGGTGGAACAAGACCCTAAAGAAATTCTTCAGTCTGTCTACGAGTGTATAGCGAGA ACGTGTGAGAAACTTGACGAACTGAATATTGATATATCCAACATAAAAGCTGTTGGTGTCAGCAATCAGA GGGAAACCACTGTAATCTGGGACAAGTTAACAGGAGAGCCTCTCTACAATGCTGTGGTGTGGCTTGATCT AAGAACCCAGACTACTGTTGAGGATCTTAGTAAAAAAATTCCAGGAAATAGTAACTTCGTCAAGTCTAAG ACAGGCCTTCCACTCAGCACTTACTTCAGTGCAGTAAAACTTCGTTGGATGCTTGACAATGTGAGAAACG TCCAAAAGGCTGTTGAAGAAGGTAGAGCTCTTTTTGGTACCATTGATTCATGGCTTATCTGGAGTTTGAC AGGAGGAGTTAATGGAGGCGTGCATTGTACAGATGTAACAAATGCAAGTAGGACAATGCTTTTTAATATC CATTCTTTGGAATGGGATAAAGAGCTCTGTGACTTTTTTGAAATTCCAATGGACCTTCTTCCAAATGTCT TCAGTTCTTCTGAGATCTATGGCCTAATTAAAACTGGAGCCCTGGAAGGTGTGCCAATATCTGGGTGTTT GGGGGACCAATGTGCTGCATTAGTAGGACAAATGTGCTTCCAGGAGGGACAAGCCAAAAACACCTATGGA ACAGGTTGCTTCTTACTGTGTAATACGGGTCGTAAATGTGTGTTTTCTGAACATGGCCTTTTGACCACAG TAGCTTACAAACTAGGCAGAGAGAAGCCAGCATATTATGCACTGGAAGGTTCTGTTGCTATAGCAGGTGC TGTTATTCGTTGGCTAAGAGACAATCTTGGAATTATAGAGACCTCAGGAGACATTGAAAGACTTGCTAAA GAAGTAGGAACTTCTTATGGCTGTTACTTTGTCCCAGCCTTTTCAGGGTTATATGCACCTTATTGGGAGC CCAGTGCAAGAGGGATACTCTGTGGCCTCACTCAGTTTACCAATAAATGTCATATTGCTTTTGCTGCATT AGAAGCTGTTTGTTTCCAAACCCGAGAGATTTTGGAAGCCATGAACCGTGACTGTGGAATTCCACTTCGT CATTTGCAGGTAGATGGAGGAATGACCAACAACAAAGTTCTTATGCAGCTACAAGCAGATATTCTTCATA TTCCAGTAATAAAACCCTTTATGCCTGAAACAACTGCACTAGGAGCTGCCATGGCAGCAGGGGCTGCAGA GGGAGTAAGCGTTTGGAGCCTTGAACCCCAGGCTTTGTCAGTTCTCAGGATGGAACGATTTGAACCACAG ATCCAGGCCACAGAAAGTGAAATTCGTTATGCCACATGGAAGAAAGCCGTAATGAAGTCAATGGGTTGGG TTACCAGTCAGTCTCCTGAAGGTGGTGATCCTTCTATCTTCTCTAGTCTGCCTTTGGGATTTTTTATAGT GAGTAGCATGGTAATGCTAATTGGAGCAAGATATATCTCGGGTGTGCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG220661 representing NM_033214
Red=Cloning site Green=Tags(s) MAAPKTAAVGPLVGAVVQGTNSTRFLVFNSKTAELLSHHKVELTQEFPKEGWVEQDPKEILQSVYECIAR TCEKLDELNIDISNIKAVGVSNQRETTVIWDKLTGEPLYNAVVWLDLRTQTTVEDLSKKIPGNSNFVKSK TGLPLSTYFSAVKLRWMLDNVRNVQKAVEEGRALFGTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNI HSLEWDKELCDFFEIPMDLLPNVFSSSEIYGLIKTGALEGVPISGCLGDQCAALVGQMCFQEGQAKNTYG TGCFLLCNTGRKCVFSEHGLLTTVAYKLGREKPAYYALEGSVAIAGAVIRWLRDNLGIIETSGDIERLAK EVGTSYGCYFVPAFSGLYAPYWEPSARGILCGLTQFTNKCHIAFAALEAVCFQTREILEAMNRDCGIPLR HLQVDGGMTNNKVLMQLQADILHIPVIKPFMPETTALGAAMAAGAAEGVSVWSLEPQALSVLRMERFEPQ IQATESEIRYATWKKAVMKSMGWVTSQSPEGGDPSIFSSLPLGFFIVSSMVMLIGARYISGVP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033214 |
| ORF Size | 1659 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033214.3 |
| RefSeq Size | 1899 bp |
| RefSeq ORF | 1662 bp |
| Locus ID | 2712 |
| UniProt ID | Q14410 |
| Domains | FGGY |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Metabolic pathways, PPAR signaling pathway |
| Gene Summary | Key enzyme in the regulation of glycerol uptake and metabolism.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220661 | GK2 (Myc-DDK-tagged)-Human glycerol kinase 2 (GK2) |
CNY 3,990.00 |
|
| RC220661L3 | Lenti-ORF clone of GK2 (Myc-DDK-tagged)-Human glycerol kinase 2 (GK2) |
CNY 5,890.00 |
|
| RC220661L4 | Lenti-ORF clone of GK2 (mGFP-tagged)-Human glycerol kinase 2 (GK2) |
CNY 5,890.00 |
|
| SC101303 | GK2 (untagged)-Human glycerol kinase 2 (GK2) |
CNY 2,950.00 |
