Products

cDNA Clones

Proteins

Expression Plasmids

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant ORF Clones

Human

Mouse

PTEN

PTPN20B

PPP2R2B

GLEPP1

PPP2R4

PPM1B

PPP2R5C

CDC25B

PTPN5

EYA1

PTPRS

PDP1

SIRP ALPHA

MTMR14

ACID PHOSPHATASE

SHP1

SKIP

PPP2R5D

PTPH1

PPP1R7

DUSP13

PPM1A

SYNAPTOJANIN

PPP2R1B

PPP3CB

PTPDC1

PTPRD

MTMR2

PCPTP1

PTP ALPHA

PTP KAPPA

CTDSP1

PPM1G

TCPTP

EYA4

CDC25C

DUSP4

PTP EPSILON

PTP4A3

PTPN7

DUSP15

SET

PPP1A

PPP6C

PTPN12

PPP2R2C

PTP4A2

PPEF1

PROSTATIC ACID PHOSPHATASE

PROTEIN PHOSPHATASE 1 BETA

PTPRN2

STYX

CD45

PP1C GAMMA

PPP2R5E

CALCINEURIN A

DEP1

DUSP19

HCE

PPP2R3A

PPP3CC

PTPN18

CDC14A

CDC25A

CTDSPL

DUSP10

DUSP6

MTMR3

PHOSPHOSERINE PHOSPHATASE

PLASTICITY RELATED GENE 3

PTP1B

PTPN22

PTPRB

CDC14B

LIPRIN ALPHA 1

PP4R1

PPP1R1C

PPP2R2D

SLINGSHOT HOMOLOG 1

PP2A-BETA

SHP2

SSH2

SYNAPTOJANIN 2

CDKN3

DUSP3

EYA3

PPP1R9B

PPP2R1A

PPP2R2A

PRY

DUSP22

EPM2A

FCP1

ILKAP

MTMR1

PNK

PP4R4

PPM1D

PPM1F

PPM1K

IHC

HEK293T

HEK293

E. coli

Sf9

Plasmids

Myc-DDK

C-Myc/DDK

Tag Free

mGFP

TurboGFP

C-MYC/DDK

N-His

C-His

N-GST and C-His

N-His&C-His

C-6His

C-6His-Avi

C-DDK

C-DDK/His

N-HIS

N-His-ABP (Albumin-Binding Protein)

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

No

Yes

LentiORF

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

SDS PAGE

Druggable Genome

Transmembrane

WB

Transcription Factors

Secreted Protein

Protein Kinase

Protease

ES Cell Differentiation/IPS

Stem cell - Pluripotency

GPCR

Phosphatase

Ion Channels: Other

Adult stem cells

Nuclear Hormone Receptor

Embryonic stem cells

Ion Channels: Potassium

Cancer stem cells

P450

Induced pluripotent stem cells

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - Wnt Signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

10 mM Tris-HCl

the solution can be further dialyzed against PBS (phosphate buffered saline

Flow Cyt

2 mM dithiothreitol

ELISA.
Western Blot.

e.g. Dulbecco's PBS).

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Western Blot.

0.1% (w/v) insulin

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

for immunoprecipitation and for Wwestern blotting.

MAPK signaling pathway

Oocyte meiosis

Wnt signaling pathway

Tight junction

Metabolic pathways

Long-term potentiation

Insulin signaling pathway

T cell receptor signaling pathway

Adherens junction

Natural killer cell mediated cytotoxicity

Inositol phosphate metabolism

Phosphatidylinositol signaling system

B cell receptor signaling pathway

Riboflavin metabolism

Focal adhesion

Cell cycle

Regulation of actin cytoskeleton

Alzheimer's disease

Amyotrophic lateral sclerosis (ALS)

Apoptosis

Axon guidance

Calcium signaling pathway

VEGF signaling pathway

Progesterone-mediated oocyte maturation

Long-term depression

TGF-beta signaling pathway

p53 signaling pathway

Fructose and mannose metabolism

Thiamine metabolism

Vascular smooth muscle contraction

Endometrial cancer

Glioma

Melanoma

Pathways in cancer

Prostate cancer

Small cell lung cancer

Jak-STAT signaling pathway

Cell adhesion molecules (CAMs)

Epithelial cell signaling in Helicobacter pylori infection

Fc gamma R-mediated phagocytosis

Primary immunodeficiency

Type I diabetes mellitus

Adipocytokine signaling pathway

Chronic myeloid leukemia

Leukocyte transendothelial migration

Neurotrophin signaling pathway

Renal cell carcinoma

Glycine

serine and threonine metabolism

Biosynthesis of unsaturated fatty acids

Sphingolipid metabolism

Human

E. coli

Human 293

Neomycin

Puromycin

None

1

2

Yes

In Stock