Products

cDNA Clones

Antibodies

Proteins

Expression Plasmids

Primary Antibodies

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant ORF Clones

Human

Mouse

CD45

PTP EPSILON

PTEN

PPM1B

CTDSP1

SIRP ALPHA

CDKN3

MTMR14

PPP2R5D

PTPN20B

PPP2R2B

CDC25B

SHP2

GLEPP1

MTMR2

PPP2R1B

SHP1

PPP2R4

PTP1B

PPP2R5C

PPP1R7

PTPN5

DUSP10

PTPN7

EYA1

DUSP5

PDP1

DUSP4

PTPRS

PPP3CB

ACID PHOSPHATASE

PTPH1

DAPP1

SKIP

DUSP13

SYNAPTOJANIN

PPP1A

DUSP1

PPM1A

PPM1G

PPP4C

PROSTATIC ACID PHOSPHATASE

PTPRD

CDC25A

CDC25C

DUSP27

PPM1D

PTPDC1

TCPTP

CALCINEURIN A

PCPTP1

PTP ALPHA

PTP KAPPA

PP1C GAMMA

PTP4A3

SET

EYA4

PPP6C

PROTEIN PHOSPHATASE 1 BETA

PTP4A2

DUSP6

DUSP15

PTPN12

PTPN22

PTPRN2

DUSP3

PPP2R2C

CDC14B

DEP1

PPEF1

PPP2R5E

DUSP19

PP2A-ALPHA

PP4R1

STYX

CDC14A

CTDSP2

CTDSPL

DUSP22

DUSP23

HCE

PHOSPHOSERINE PHOSPHATASE

PP2A-BETA

PPP3CC

LIPRIN ALPHA 1

PP4R4

PPM1F

PPP1R1C

PPP2R1A

PPP2R2D

PPP2R3A

PTPN18

MTMR3

PLASTICITY RELATED GENE 3

PTPRB

RPTP MU

DUSP12

MTMR6

MTMR7

PNK

Human

Mouse

Rat

Dog

Bovine

Cow)

Monkey

Human (Expected from sequence similarity: Mouse

Human (Predicted: Mouse

Pig

Bovine)

Canine

Rabbit

Rat (Expected from sequence similarity: Dog

X. tropicalis)

Chicken

Dog)

Rat)

Rhesus Monkey)

Mouse (Predicted: Rat

Mouse (Predicted: Rhesus Monkey)

Rabbit)

Xenopus

Bat

Chimpanzee

Cow

Gibbon

Hamster

Horse (Predicted: Monkey

Human (Expected from sequence similarity: Cow)

Human (Expected from sequence similarity: Dog

Human (Expected from sequence similarity: Rat

Human (Predicted: Bovine)

Human (Predicted: Mouse)

Human (Predicted: Pig

Human (Predicted: Rabbit)

Human (Predicted: Rat

Mouse (Expected from sequence similarity: Rat

Mouse (Expected from sequence similarity: Rat)

Mouse (Predicted: Bovine

Mouse (Predicted: Chicken

Pig (Expected from sequence similarity: Dog

Pig)

Porcine

Rat (Expected from sequence similarity: Dog)

Rat (Expected from sequence similarity: Human

Rhesus Monkey

Xenopus)

Zebrafish

WB

IHC

FC

IF

ELISA

ICC/IF

IP

Dot

ID

PEP-ELISA

R

Mouse

Rabbit

Goat

Sheep

Rat

HEK293T

HEK293

E. coli

Sf9

Plasmids

Myc-DDK

C-Myc/DDK

Tag Free

mGFP

TurboGFP

C-MYC/DDK

N-His

C-His

N-GST and C-His

C-6His

N-His&C-His

C-6His-Avi

C-DDK

C-DDK/His

N-HIS

N-His-ABP (Albumin-Binding Protein)

pCMV6-Entry

pCMV6-AC-GFP

pLenti-C-Myc-DDK-P2A-Puro

pLenti-C-mGFP-P2A-Puro

pLenti-C-Myc-DDK

pLenti-C-mGFP

pCMV6-XL5

pCMV6-XL4

pCMV6-AC

pCMV6-XL6

PCMV6-Neo

KO Validated Antibodies

Unconjugated

Biotin

HRP

FITC

PE

APC

PerCP

Phospho-specific

IgG1

IgG

IgG2a

IgG2b

Ig

IgG1, kappa

IgG2a, kappa

Monoclonal

Polyclonal

No

Yes

Indirect

Direct

LentiORF

TrueMAB

TrueORF

TrueClone

MVPro

TrueORF Gold, TrueORF

UltraMAB

SDS PAGE

Druggable Genome

Transmembrane

Transcription Factors

WB

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

GPCR

Phosphatase

Adult stem cells

Ion Channels: Other

Nuclear Hormone Receptor

Embryonic stem cells

Cancer stem cells

Induced pluripotent stem cells

Ion Channels: Potassium

P450

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Ion Channels: Cys-loop Receptors

Stem cell relevant signaling - TGFb/BMP signaling pathway

Ion Channels: Transient receptor potential

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Glutamate Receptors

Ion Channels: Calcium

Ion Channels: Sodium

Ion Channels: ATP Receptors

Ion Channels: Cyclic nucleotide gated

ELISA

SDS-PAGE

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

ELISA.
Western blotting.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues

Secreted

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

MAPK signaling pathway

Oocyte meiosis

Wnt signaling pathway

Tight junction

T cell receptor signaling pathway

Natural killer cell mediated cytotoxicity

Insulin signaling pathway

Metabolic pathways

Long-term potentiation

Adherens junction

B cell receptor signaling pathway

Focal adhesion

Inositol phosphate metabolism

Phosphatidylinositol signaling system

Riboflavin metabolism

Cell adhesion molecules (CAMs)

Cell cycle

Fc gamma R-mediated phagocytosis

Primary immunodeficiency

Regulation of actin cytoskeleton

Progesterone-mediated oocyte maturation

p53 signaling pathway

Alzheimer's disease

Amyotrophic lateral sclerosis (ALS)

Apoptosis

Axon guidance

Calcium signaling pathway

VEGF signaling pathway

Long-term depression

TGF-beta signaling pathway

Jak-STAT signaling pathway

Fructose and mannose metabolism

Thiamine metabolism

Vascular smooth muscle contraction

Endometrial cancer

Glioma

Melanoma

Pathways in cancer

Prostate cancer

Small cell lung cancer

Epithelial cell signaling in Helicobacter pylori infection

Adipocytokine signaling pathway

Chronic myeloid leukemia

Leukocyte transendothelial migration

Neurotrophin signaling pathway

Renal cell carcinoma

Type I diabetes mellitus

Glycine

serine and threonine metabolism

Biosynthesis of unsaturated fatty acids

Sphingolipid metabolism

Human

E. coli

Human 293

Neomycin

Puromycin

None

Carrier-free Antibodies

1

2

3

Yes

In Stock