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Mad2l2 (NM_001012106) Rat Tagged ORF Clone
CAT＃: RR210119
- TrueORF®
Mad2l2 (Myc-DDK-tagged ORF) - Rat MAD2 mitotic arrest deficient-like 2 (yeast) (Mad2l2), (10 ug)
"NM_001012106" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR210119 representing NM_001012106
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACCCTCACGCGCCAAGACCTCAACTTCGGCCAAGTGGTGGCCGACGTGCTCTCCGAGTTCCTGG AGGTGGCCGTGCACTTGATCCTCTATGTGCGCGAGGTCTACCCGGTGGGCATCTTTCAGAAGCGCAAGAA GTACAACGTGCCGGTTCAGATGTCCTGCCACCCAGAGCTGAACCAGTACATCCAGGACACACTTCACTGC GTCAAACCACTCCTGGAGAAGAACGATGTGGAGAAGGTGGTGGTGGTGATTTTGGATAAGGAACACCGCC CAGTGGAGAAGTTTGTCTTTGAGATCACTCAGCCACCCTTGCTGTCCATCAAGCTAGGTGGCCTCAAACA GACCGTTTCTCTAACTGTCTTGGGGTCCTGGAGGTATGCAGACTTGAGCGGTACCCTGGGTGTCCTCTCG ACAGCTGAATATTTGAGTGACCTAAAGGGACCTTTGTACCCTACCTCTATTCAGTCAGTTCAGACTCCCT CCTGTCTCATGTGGAGCAGCTGCTTCGAGCCTTCATTCTTAAGATTAGTGTGTGTGACGCTGTCCTCGAC CATAATCCTCCAGGCTGCACGTTTACAGTTCTCGTGCACACAAGAGAAGCTGCCACTCGGAACATGGAGA AGATACAGGTCATCAAGGACTTCCCCTGGATCCTGGCAGATGAGCAAGATGTCCACATGCATGATCCCCG GT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR210119 representing NM_001012106
Red=Cloning site Green=Tags(s) MTTLTRQDLNFGQVVADVLSEFLEVAVHLILYVREVYPVGIFQKRKKYNVPVQMSCHPELNQYIQDTLHC VKPLLEKNDVEKVVVVILDKEHRPVEKFVFEITQPPLLSIKLGGLKQTVSLTVLGSWRYADLSGTLGVLS TAEYLSDLKGPLYPTSIQSVQTPSCLMWSSCFEPSFLRLVCVTLSSTIILQAARLQFSCTQEKLPLGTWR RYRSSRTSPGSWQMSKMSTCMIPG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001012106 |
| ORF Size | 702 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001012106.1, NP_001012106.1 |
| RefSeq Size | 1188 bp |
| RefSeq ORF | 705 bp |
| Locus ID | 313702 |
| UniProt ID | D3Z8D9 |
| MW | 26.4 kDa |
| Gene Summary | Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs). During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection. Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN210119 | Mad2l2 (untagged ORF) - Rat MAD2 mitotic arrest deficient-like 2 (yeast) (Mad2l2), (10 ug) |
CNY 3,990.00 |
|
| RR210119L3 | Lenti ORF clone of Mad2l2 (Myc-DDK-tagged ORF) - Rat MAD2 mitotic arrest deficient-like 2 (yeast) (Mad2l2), (10 ug) |
CNY 6,080.00 |
|
| RR210119L4 | Lenti ORF clone of Mad2l2 (mGFP-tagged ORF) - Rat MAD2 mitotic arrest deficient-like 2 (yeast) (Mad2l2), (10 ug) |
CNY 6,650.00 |
