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QRICH1 (NM_017730) Human Tagged ORF Clone
CAT＃: RC213114
QRICH1 (Myc-DDK-tagged)-Human glutamine-rich 1 (QRICH1), transcript variant 1
ORF Plasmid: tGFP
"NM_017730" in other vectors (7)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AB-DIP; VERBRAS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213114 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATAATTCCCTAGAGAACACCATCTCCTTTGAAGAGTACATCCGAGTAAAGGCACGGTCTGTCCCGC AACACAGGATGAAGGAATTTCTGGACTCACTGGCCTCTAAGGGGCCAGAAGCCCTTCAGGAGTTCCAGCA GACAGCCACCACTACCATGGTGTACCAACAGGGTGGGAACTGCATATACACAGACAGCACTGAAGTGGCT GGGTCTTTGCTTGAACTTGCCTGTCCAGTCACCACCAGTGTTCAGCCACAAACCCAGCAAGAACAGCAGA TCCAGGTTCAGCAGCCGCAGCAGGTTCAGGTCCAGGTGCAGGTACAGCAGTCTCCGCAACAGGTCTCGGC TCAGCTCTCCCCACAACTCACCGTTCACCAGCCTACTGAGCAACCCATCCAGGTCCAGGTGCAGATCCAA GGCCAGGCACCACAGTCAGCAGCCCCCTCCATTCAGACCCCGTCTCTGCAGAGTCCCAGTCCCTCGCAGC TGCAAGCAGCTCAGATCCAGGTGCAGCACGTGCAAGCAGCCCAGCAGATCCAGGCTGCAGAAATCCCGGA GGAGCACATCCCACATCAGCAAATCCAGGCTCAGCTGGTGGCTGGCCAGTCTCTTGCTGGTGGTCAGCAG ATCCAAATCCAGACCGTGGGTGCCCTTTCCCCACCACCATCCCAGCAGGGCTCACCCCGGGAAGGGGAGC GGCGGGTTGGCACGGCCAGTGTCCTCCAACCAGTGAAGAAGCGCAAAGTGGACATGCCCATCACTGTGTC CTACGCCATCTCAGGGCAGCCGGTGGCCACCGTGCTGGCCATTCCACAGGGCCAGCAGCAGAGTTATGTG TCTTTGAGGCCAGACTTACTGACAGTAGACAGTGCCCACCTGTACAGTGCCACTGGGACCATTACTAGCC CTACAGGAGAAACCTGGACCATCCCTGTTTATTCTGCCCAGCCCCGGGGGGACCCTCAGCAGCAGAGCAT TACCCACATTGCCATTCCCCAGGAAGCCTACAACGCAGTTCACGTCAGTGGCTCACCCACAGCCCTGGCA GCTGTTAAGCTGGAGGATGACAAGGAGAAGATGGTGGGCACCACATCTGTAGTGAAAAACTCCCATGAAG AGGTAGTGCAGACCCTTGCAAACTCTCTCTTTCCAGCACAGTTCATGAATGGCAACATCCACATTCCAGT GGCTGTGCAGGCTGTGGCAGGCACGTACCAGAATACGGCTCAAACTGTCCATATATGGGACCCCCAACAG CAGCCGCAGCAGCAAACTCCCCAGGAACAGACACCACCACCACAGCAGCAGCAGCAGCAACTCCAAGTTA CTTGTTCAGCTCAAACTGTCCAGGTTGCTGAAGTTGAACCACAGTCACAGCCACAGCCTTCCCCAGAACT TCTGCTTCCAAATTCTTTGAAGCCAGAAGAAGGGCTTGAAGTATGGAAAAACTGGGCCCAGACCAAGAAT GCTGAACTAGAGAAGGATGCTCAGAACAGATTGGCACCCATTGGGAGGCGCCAACTGCTGCGATTCCAGG AAGATCTCATCTCCTCTGCTGTGGCAGAGTTGAATTATGGGCTCTGTCTAATGACACGGGAAGCTCGAAA TGGAGAAGGTGAACCCTATGACCCAGATGTGCTCTACTATATTTTCCTGTGTATTCAAAAGTATCTTTTT GAAAATGGAAGGGTAGATGACATTTTCTCCGATCTTTATTATGTTCGGTTCACGGAGTGGCTACATGAAG TTCTGAAGGATGTTCAGCCCCGGGTCACTCCACTTGGCTATGTCTTGCCCAGCCACGTGACTGAGGAGAT GCTATGGGAGTGCAAGCAGCTTGGGGCTCACTCCCCCTCCACCTTGCTGACCACCCTCATGTTCTTTAAT ACCAAGTACTTCCTATTGAAGACAGTGGACCAGCACATGAAGCTGGCCTTCTCCAAGGTCTTGCGACAGA CAAAGAAGAACCCCTCTAATCCCAAGGATAAAAGCACGAGTATCCGGTACTTGAAGGCCCTTGGAATACA CCAGACTGGCCAGAAAGTTACAGATGACATGTATGCAGAACAGACGGAAAATCCAGAGAATCCATTGAGA TGTCCCATCAAGCTCTATGATTTCTACCTCTTCAAATGCCCCCAGAGTGTGAAAGGCCGGAATGACACCT TTTACCTGACACCTGAGCCAGTGGTGGCCCCCAACAGCCCAATCTGGTACTCAGTCCAGCCTATCAGCAG AGAGCAGATGGGACAAATGCTGACGCGGATCCTGGTGATAAGAGAAATTCAGGAGGCCATCGCAGTGGCC AATGCAAGCACTATGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213114 protein sequence
Red=Cloning site Green=Tags(s) MNNSLENTISFEEYIRVKARSVPQHRMKEFLDSLASKGPEALQEFQQTATTTMVYQQGGNCIYTDSTEVA GSLLELACPVTTSVQPQTQQEQQIQVQQPQQVQVQVQVQQSPQQVSAQLSPQLTVHQPTEQPIQVQVQIQ GQAPQSAAPSIQTPSLQSPSPSQLQAAQIQVQHVQAAQQIQAAEIPEEHIPHQQIQAQLVAGQSLAGGQQ IQIQTVGALSPPPSQQGSPREGERRVGTASVLQPVKKRKVDMPITVSYAISGQPVATVLAIPQGQQQSYV SLRPDLLTVDSAHLYSATGTITSPTGETWTIPVYSAQPRGDPQQQSITHIAIPQEAYNAVHVSGSPTALA AVKLEDDKEKMVGTTSVVKNSHEEVVQTLANSLFPAQFMNGNIHIPVAVQAVAGTYQNTAQTVHIWDPQQ QPQQQTPQEQTPPPQQQQQQLQVTCSAQTVQVAEVEPQSQPQPSPELLLPNSLKPEEGLEVWKNWAQTKN AELEKDAQNRLAPIGRRQLLRFQEDLISSAVAELNYGLCLMTREARNGEGEPYDPDVLYYIFLCIQKYLF ENGRVDDIFSDLYYVRFTEWLHEVLKDVQPRVTPLGYVLPSHVTEEMLWECKQLGAHSPSTLLTTLMFFN TKYFLLKTVDQHMKLAFSKVLRQTKKNPSNPKDKSTSIRYLKALGIHQTGQKVTDDMYAEQTENPENPLR CPIKLYDFYLFKCPQSVKGRNDTFYLTPEPVVAPNSPIWYSVQPISREQMGQMLTRILVIREIQEAIAVA NASTMH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017730 |
| ORF Size | 2328 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017730.3, NP_060200.2 |
| RefSeq Size | 3331 bp |
| RefSeq ORF | 2331 bp |
| Locus ID | 54870 |
| UniProt ID | Q2TAL8 |
| Protein Families | Druggable Genome |
| MW | 86.4 kDa |
| Gene Summary | Transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PubMed:33384352). Controls the outcome of the unfolded protein response (UPR) which is an ER-stress response pathway (PubMed:33384352). ER stress induces QRICH1 translation by a ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced QRICH1 regulates a transcriptional program associated with protein translation, protein secretion-mediated proteotoxicity and cell death during the terminal UPR (PubMed:33384352). May cooperate with ATF4 transcription factor signaling to regulate ER homeostasis which is critical for cell viability (PubMed:33384352). Upregulates CASP3/caspase-3 activity in epithelial cells under ER stress. Central regulator of proteotoxicity associated with ER stress-mediated inflammatory diseases in the intestines and liver (PubMed:33384352). Involved in chondrocyte hypertrophy, a process required for normal longitudinal bone growth (PubMed:30281152).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213114L1 | Lenti ORF clone of Human glutamine-rich 1 (QRICH1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213114L2 | Lenti ORF clone of Human glutamine-rich 1 (QRICH1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC213114L3 | Lenti ORF clone of Human glutamine-rich 1 (QRICH1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213114L4 | Lenti ORF clone of Human glutamine-rich 1 (QRICH1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG213114 | QRICH1 (tGFP-tagged) - Human glutamine-rich 1 (QRICH1), transcript variant 1 |
CNY 4,370.00 |
|
| SC111309 | QRICH1 (untagged)-Human glutamine-rich 1 (QRICH1), transcript variant 1 |
CNY 3,656.00 |
|
| SC318260 | QRICH1 (untagged)-Human glutamine-rich 1 (QRICH1), transcript variant 1 |
CNY 7,220.00 |
