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LOC729020 (Myc-DDK-tagged)-Human rcRPE (LOC729020)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
RPEL1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
LOC729020 (tGFP-tagged) - Human rcRPE (LOC729020)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Human rcRPE (LOC729020), mGFP tagged
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
RPEL1 CRISPRa kit - CRISPR gene activation of human ribulose-5-phosphate-3-epimerase like 1
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Lenti ORF clone of Human rcRPE (LOC729020), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Transient overexpression lysate of rcRPE (LOC729020)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
RPEL1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
RPEL1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Transient overexpression of RPEL1 in HEK293T cells, FFPE control for IHC, ICC and ISH staining, 25 slides per pack
RPEL1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
LOC729020 (untagged)-Human rcRPE (LOC729020)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
qSTAR qPCR primer pairs against Homo sapiens gene RPEL1
3' UTR clone of rcRPE (LOC729020) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
