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Nat6 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Products
Naa80 CRISPRa kit - CRISPR gene activation of mouse N(alpha)-acetyltransferase 80, NatH catalytic subunit
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
NAA80 CRISPRa kit - CRISPR gene activation of human N(alpha)-acetyltransferase 80, NatH catalytic subunit
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Nat6 - Mouse, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
NAT6 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
NAT6 / FUS2 (1-308, His-tag) human recombinant protein, 50 µg
| Tag | His-tag |
| Expression Host | E. coli |
NAT6 / FUS2 (1-308, His-tag) human recombinant protein, 10 µg
| Tag | His-tag |
| Expression Host | E. coli |
qPCR primer pairs and template standards against Homo sapiens gene NAT6
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene NAT6
| Application | Plasmid of exact quantity for transcript copy number calculation |
NAT6 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
NAT6 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Nat6 - Mouse, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
Nat6 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
3' UTR clone of N-acetyltransferase 6 (GCN5-related) (NAT6) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene NAT6
