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SUFU CRISPRa kit - CRISPR gene activation of human SUFU negative regulator of hedgehog signaling
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
SUFU / SUFUH (1-484, His-tag) human recombinant protein, 0.5 mg
| Tag | His-tag |
| Expression Host | E. coli |
SUFU / SUFUH (1-484, His-tag) human recombinant protein, 0.1 mg
| Tag | His-tag |
| Expression Host | E. coli |
qPCR primer pairs and template standards against Homo sapiens gene SUFU
| Application | Plasmid of exact quantity for transcript copy number calculation |
SUFU - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
SUFU - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
SUFU (untagged)-Human suppressor of fused homolog (Drosophila) (SUFU) transcript variant 2
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
qSTAR qPCR primer pairs against Homo sapiens gene SUFU
SUFU (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
