Products

RB1 Mutant (R455*), Myc-DDK-tagged ORF clone of Homo sapiens retinoblastoma 1 (RB1) as transfection-ready DNA

Mutation R455*
Tag Myc-DDK
Vector pCMV6-Entry
  • TrueORF®

RB1 Mutant (R251*), Myc-DDK-tagged ORF clone of Homo sapiens retinoblastoma 1 (RB1) as transfection-ready DNA

Mutation R251*
Tag Myc-DDK
Vector pCMV6-Entry
  • TrueORF®

RB1 Mutant (R552*), Myc-DDK-tagged ORF clone of Homo sapiens retinoblastoma 1 (RB1) as transfection-ready DNA

Mutation R552*
Tag Myc-DDK
Vector pCMV6-Entry
  • TrueORF®

RB1 Mutant (R556*), Myc-DDK-tagged ORF clone of Homo sapiens retinoblastoma 1 (RB1) as transfection-ready DNA

Mutation R556*
Tag Myc-DDK
Vector pCMV6-Entry
  • TrueORF®

RB1 Mutant (R661W), Myc-DDK-tagged ORF clone of Homo sapiens retinoblastoma 1 (RB1) as transfection-ready DNA

Mutation R661W
Tag Myc-DDK
Vector pCMV6-Entry
  • TrueORF®

RB1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti

Transient overexpression lysate of retinoblastoma 1 (RB1)

Tag C-Myc/DDK
Expression Host HEK293T

RB1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

RB1 CRISPRa kit - CRISPR gene activation of human RB transcriptional corepressor 1

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

RB1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

qSTAR qPCR primer pairs against Homo sapiens gene RB1

RB1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRFP-C-RS

qPCR primer pairs and template standards against Homo sapiens gene RB1

Application Plasmid of exact quantity for transcript copy number calculation

3' UTR clone of retinoblastoma 1 (RB1) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase