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PRKCH Mutant (v374I), Myc-DDK-tagged ORF clone of Homo sapiens protein kinase C, eta (PRKCH) as transfection-ready DNA
| Mutation | v374I |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Products
PRKCH CRISPRa kit - CRISPR gene activation of human protein kinase C eta
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
PRKCH (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
PRKCH - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
PRKCH - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
PRKCH - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
qPCR primer pairs and template standards against Homo sapiens gene PRKCH
| Application | Plasmid of exact quantity for transcript copy number calculation |
3' UTR clone of protein kinase C eta (PRKCH) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene PRKCH
