Products

PRKAB1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pGFP-V-RS

AMPK beta-1 chain / AMPKb (1-270, His-tag) human recombinant protein, 10 µg

Tag His-tag
Expression Host E. coli

PRKAB1 CRISPRa kit - CRISPR gene activation of human protein kinase AMP-activated non-catalytic subunit beta 1

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

AMPK beta-1 chain / AMPKb (1-270, His-tag) human recombinant protein, 50 µg

Tag His-tag
Expression Host E. coli

qPCR primer pairs and template standards against Homo sapiens gene PRKAB1

Application Plasmid of exact quantity for transcript copy number calculation

PRKAB1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

3' UTR clone of protein kinase AMP-activated beta 1 non-catalytic subunit (PRKAB1) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

PRKAB1 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
SR321411 is the updated version of SR303723.