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PPP1R1B CRISPRa kit - CRISPR gene activation of human protein phosphatase 1 regulatory inhibitor subunit 1B
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
PPP1R1B (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene PPP1R1B
PPP1R1B - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
PPP1R1B - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pGFP-V-RS |
qPCR primer pairs and template standards against Homo sapiens gene PPP1R1B
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene PPP1R1B
| Application | Plasmid of exact quantity for transcript copy number calculation |
3' UTR clone of protein phosphatase 1 regulatory (inhibitor) subunit 1B (PPP1R1B) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
PPP1R1B - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
3' UTR clone of protein phosphatase 1 regulatory (inhibitor) subunit 1B (PPP1R1B) transcript variant 2 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
