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POLR2J3 CRISPRa kit - CRISPR gene activation of human RNA polymerase II subunit J3
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
POLR2J3 (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide J3 (POLR2J3)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
POLR2J3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
POLR2J3 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
3' UTR clone of polymerase (RNA) II (DNA directed) polypeptide J3 (POLR2J3) for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene POLR2J3
POLR2J3 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
