Products

POLR2I CRISPRa kit - CRISPR gene activation of human RNA polymerase II subunit I

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

POLR2I (1-125, His-tag) human recombinant protein, 50 µg

Tag His-tag
Expression Host E. coli

POLR2I (1-125, His-tag) human recombinant protein, 0.25 mg

Tag His-tag
Expression Host E. coli

3' UTR clone of polymerase (RNA) II (DNA directed) polypeptide I 14.5kDa (POLR2I) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

qPCR primer pairs and template standards against Homo sapiens gene POLR2I

Application Plasmid of exact quantity for transcript copy number calculation

POLR2I (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).