Products

Prohibitin / PHB (1-272, His-tag) human recombinant protein, 50 µg

Tag His-tag
Expression Host E. coli

PHB CRISPRa kit - CRISPR gene activation of human prohibitin

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Prohibitin / PHB (1-272, His-tag) human recombinant protein, 0.25 mg

Tag His-tag
Expression Host E. coli

PHB (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
SR321292 is the updated version of SR303488.

PHB - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

PHB - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRFP-C-RS

qPCR primer pairs and template standards against Homo sapiens gene PHB

Application Plasmid of exact quantity for transcript copy number calculation

PHB - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Format Lentiviral plasmids
Vector pGFP-C-shLenti

3' UTR clone of prohibitin (PHB) for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase