Products

Myelin Basic Protein (1-197, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

MBP CRISPRa kit - CRISPR gene activation of human myelin basic protein

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Myelin Basic Protein (1-197, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

qPCR primer pairs and template standards against Homo sapiens gene MBP

Application Plasmid of exact quantity for transcript copy number calculation

MBP - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

MBP (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).

3' UTR clone of myelin basic protein (MBP) transcript variant 3 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

3' UTR clone of myelin basic protein (MBP) transcript variant 7 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

3' UTR clone of myelin basic protein (MBP) transcript variant 1 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

3' UTR clone of myelin basic protein (MBP) transcript variant 4 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

3' UTR clone of myelin basic protein (MBP) transcript variant 2 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase