United States
Germany
Japan
United Kingdom
China
KRT20 CRISPRa kit - CRISPR gene activation of human keratin 20
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
KRT20 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene KRT20
qPCR primer pairs and template standards against Homo sapiens gene KRT20
| Application | Plasmid of exact quantity for transcript copy number calculation |
KRT20 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Cytokeratin 20 human recombinant protein, 0.25 mg
| Expression Host | E. coli |
Cytokeratin 20 human recombinant protein, 0.1 mg
| Expression Host | E. coli |
KRT20 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
活动过滤器
优化搜索结果
Expression Hosts
Lentivirus
Vectors
Reporters
Families
- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (2) - both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- e.g. Dulbecco's PBS). (2)
- pH 7.4). For immunization purposes (2)
- the solution can be further dialyzed against PBS (phosphate buffered saline (2)
