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Cytokeratin 19 human recombinant protein, 0.1 mg
| Expression Host | E. coli |
Products
KRT19 CRISPRa kit - CRISPR gene activation of human keratin 19
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qSTAR qPCR primer pairs against Homo sapiens gene KRT19
KRT19 (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Homo sapiens gene KRT19
| Application | Plasmid of exact quantity for transcript copy number calculation |
KRT19 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
Cytokeratin 19 human recombinant protein, 0.25 mg
| Expression Host | E. coli |
KRT19 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
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Lentivirus
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- 10 mM Tris-HCl (2)
- 2 mM dithiothreitol (2)
-
Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml (2) - both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- e.g. Dulbeccos PBS). (2)
-
pH 7.4).
For immunization purposes (2) - the solution can be further dialyzed against PBS (phosphate buffered saline (2)
