Products

HIF1A CRISPRa kit - CRISPR gene activation of human hypoxia inducible factor 1 subunit alpha

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

3' UTR clone of hypoxia inducible factor 1 alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) transcript variant 1 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

qPCR primer pairs and template standards against Homo sapiens gene HIF1A

Application Plasmid of exact quantity for transcript copy number calculation

HIF1A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).
SR320469 is the updated version of SR302102.

HIF1A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pGFP-V-RS

HIF1A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

HIF1A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRFP-C-RS

3' UTR clone of hypoxia inducible factor 1 alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) transcript variant 2 for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase