qSTAR qPCR primer pairs against Homo sapiens gene HIF1A
Products
HIF1A CRISPRa kit - CRISPR gene activation of human hypoxia inducible factor 1 subunit alpha
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
3' UTR clone of hypoxia inducible factor 1 alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) transcript variant 1 for miRNA target validation
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
qPCR primer pairs and template standards against Homo sapiens gene HIF1A
Application | Plasmid of exact quantity for transcript copy number calculation |
HIF1A - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |
HIF1A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
HIF1A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pGFP-V-RS |
HIF1A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRS |
HIF1A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
Format | Retroviral plasmids |
Vector | pRFP-C-RS |
3' UTR clone of hypoxia inducible factor 1 alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) transcript variant 2 for miRNA target validation
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |