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DST CRISPRa kit - CRISPR gene activation of human dystonin
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
qSTAR qPCR primer pairs against Homo sapiens gene DST
3' UTR clone of dystonin (DST) transcript variant 1e for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
3' UTR clone of dystonin (DST) transcript variant 1eA for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
DST (untagged)-Human dystonin (DST), transcript variant 1eA
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
DST - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
DST - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
DST (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
DST (untagged)-Human dystonin (DST), transcript variant 1
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
(untagged)-Human dystonin isoform 1 mRNA, partial cds
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
(untagged)-Human cDNA: FLJ21489 fis, clone COL05450
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6 series |
