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DFFA CRISPRa kit - CRISPR gene activation of human DNA fragmentation factor subunit alpha
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Products
DFFA / ICAD (1-331, His-tag) human recombinant protein, 0.5 mg
| Tag | His-tag |
| Expression Host | E. coli |
DFFA (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qPCR primer pairs and template standards against Homo sapiens gene DFFA
| Application | Plasmid of exact quantity for transcript copy number calculation |
qPCR primer pairs and template standards against Homo sapiens gene DFFA
| Application | Plasmid of exact quantity for transcript copy number calculation |
DFFA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
DFFA - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
3' UTR clone of DNA fragmentation factor 45kDa alpha polypeptide (DFFA) transcript variant 1 for miRNA target validation
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
qSTAR qPCR primer pairs against Homo sapiens gene DFFA
