Products

CS CRISPRa kit - CRISPR gene activation of human citrate synthase

Format 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)

Citrate synthase (28-466, His-tag) human recombinant protein, 0.1 mg

Tag His-tag
Expression Host E. coli

Citrate synthase (28-466, His-tag) human recombinant protein, 0.5 mg

Tag His-tag
Expression Host E. coli

qPCR primer pairs and template standards against Homo sapiens gene CS

Application Plasmid of exact quantity for transcript copy number calculation

qPCR primer pairs and template standards against Homo sapiens gene CS

Application Plasmid of exact quantity for transcript copy number calculation

CS - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Format Retroviral plasmids
Vector pRS

3' UTR clone of citrate synthase (CS) nuclear gene encoding mitochondrial protein for miRNA target validation

Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase

CS (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each

Purity HPLC purified
Number of Transfections Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM).